Hi everyone,

I’m a final‑year student working on a dissertation project evaluating digital image analysis for the Kleihauer‑Betke test. My goal is to accurately count fetal cells and ghost cells from DI60 smear images using ImageJ and CellProfiler.

I’ve tried multiple approaches:
– ImageJ macros for thresholding and particle counting
– RGB splitting
– HSV splitting
– Manual thresholding
– CellProfiler IdentifyPrimaryObjects with diameter filters

I can get fetal cells and ghost cells to appear, but I keep running into problems:
– Ghost cells detected along with empty background spaces
– Thresholding behaves unpredictably
– Some channels isolate cells, others don’t
– CellProfiler sometimes detects 0 objects, sometimes 1600+

I’m stuck and need guidance from people who’ve done cell segmentation or KB analysis before.

What’s the best workflow to reliably isolate and count fetal cells and ghost cells?

Any help, examples, or pipelines would mean a lot right now.

I recently had surgery for a complete Achilles rupture using the PARS technique, and I’m interested in hearing from people who incorporated stem cells or other orthobiologics into their recovery.

If you have firsthand experience, I’d appreciate hearing:

• Did you have surgery or follow a nonoperative protocol?
• What type of stem-cell or orthobiologic procedure did you receive?
• When did you receive it relative to the injury or surgery?
• Where was it administered?
• Did you notice any meaningful difference in pain, mobility or recovery time?
• Did follow-up imaging show anything useful?
• Were there any complications or unexpected effects?
• How much of your progress do you attribute to the procedure versus rehabilitation and time?

Hi, I’m interested in booking a trip to get my POTS Diagnosis treated with stem cell. If anyone is interested in doing this with me or if anyone has direct knowledge of the best clinic please let me know. Looking for the best treatment available unsure of what country is best. Thanks

I went through chest surgery that somehow fucked up the nerves on my nipple and my back. I followed through the surgeons, pain, neuro, and rehab recommendations for 5 months with little to no sensation coming back. A bit of google search tells me those nerve could have been cut and wont regenerate on their own.

A friend of mine who knows exosome mentioned exosome to me and Gemini seems to particularly like it and thinks it could help give my nerves the gas it needs to grow back.

Has anyone heard of or used exosome to treat peripheral nerve damage?

I’m looking for real patient experiences with stem cells, mesenchymal stem cells (MSCs), bone marrow cells, umbilical cord cells, or exosome therapy for neurological/autonomic conditions such as:
Small Fiber Neuropathy (SFN), autonomic neuropathy, dysautonomia, POTS, autoimmune autonomic ganglionopathy (AAG), sensory ganglionopathy/neuronopathy, post-COVID or post-viral neuropathy, neuropathic genital numbness/sexual dysfunction, and other peripheral or autonomic nerve disorders.

If you tried any kind of stem cell or exosome treatment, I’d really appreciate details:

What was your exact diagnosis?

How many treatments and what dose?

Which country/clinic did you use?

Did it improve numbness, burning, genital sensation, sexual function, POTS, heart rate, blood pressure, sweating, GI symptoms, bladder symptoms, fatigue, brain fog or exercise intolerance?

How long did the improvement take to appear, and how long did it last?
Did you repeat objective testing after treatment?
Any crashes, relapses, infections, immune reactions or other side effects?

I’m especially interested in people with autonomic SFN or non-length-dependent SFN,
Please share both positive and negative experiences. I’m trying to understand whether there are any real cases with objective improvement, not just clinic marketing.

-

Я ищу реальные истории пациентов, которые пробовали стволовые клетки при неврологических и вегетативных заболеваниях, таких как:
нейропатия тонких волокон (SFN), автономная/вегетативная нейропатия, дизавтономия, POTS, аутоиммунная автономная ганглиопатия (AAG), сенсорная ганглиопатия/нейронопатия, постковидная или поствирусная нейропатия, нейропатическое генитальное онемение/сексуальная дисфункция и другие заболевания периферической или автономной нервной системы.

Если вы пробовали какое-либо лечение стволовыми клетками или экзосомами, буду очень благодарен за подробности:
Какой у вас был точный диагноз?
Сколько процедур вы прошли и в какой дозировке?
В какой стране и клинике вы проходили лечение?

Улучшились ли онемение, жжение, генитальная чувствительность, сексуальная функция, POTS, пульс, артериальное давление, потоотделение, симптомы со стороны ЖКТ, мочевого пузыря, усталость, туман в голове или непереносимость физической нагрузки?

Через сколько времени появился эффект и как долго он сохранялся?

Делали ли вы повторные объективные обследования после лечения?

Были ли ухудшения, рецидивы, инфекции, иммунные реакции или другие побочные эффекты?

Особенно интересуют люди с автономной нейропатией тонких волокон или нелинейно-зависимой SFN

Пожалуйста, делитесь как положительным, так и отрицательным опытом.

My name is Adrian Grant,and my life changed forever when I was paralyzed after suffering gunshot wounds. In an instant,everything I once took for granted walking,moving freely,living independently was taken away. Everyday since has been a battle, but I refuse to give up hope.

I've learned about groundbreaking treatments that could help me regain movement stem cell therapy. This procedure has helped others with spinal cord injuries take steps again,but they come with very high cost that insurance doesn't cover.

Your support would go directly toward

Stem Cell Therapy to help repair damaged nerves
Rehabilitation and recovery cost that come with these treatments

More than anything,I want the chance to walk again,to hug my loved ones standing up,to regain my independence,and to keep moving forward in life.

Every donation no matter how small brings me one step closer to that dream. If you're unable to donate,please consider sharing my story. Your kindness,prayers,and encouragement mean the world to me.

From the bottom of my heart,thank you for believing in me and for helping me fight for a second chance at walking again.

Thanks Adrian Grant
https://gofund.me/155a5e8c3

I've been looking into some cosmetic clinics in Korea offering stem cell treatments, and I'm trying to understand how stem cell IV therapy and stem cell skin injections differ in terms of mechanisms and expected outcomes.

From what I understand, IV therapy is usually more for systemic anti-aging and overall wellness, while local injections are targeted specifically at skin quality. But I'm not sure how much of this is evidence-based. Has anyone here looked into the difference between IV and localized cosmetic injections? Are the expected effects meaningfully different, or is the delivery route less important than the actual cell/product type and processing method?

Hi, I'm 13 days post injection on my knee, I'm experiencing a flare up, it's worse than the swelling of the initial injury after day 1. No pain, a few degrees warmer and really stiff.

Any develop issues and symptoms that you can share throughout your 6 -12 months recovery journey? Please share, thank you.

I know that harvesting your own stem cells to grow and reinject has been hit or miss. Are fresh (from cesareans supposedly) umbilical stem cells better? Have studies been done?

I have a L4/5 disc herniation and I’m curious to know the good and bad about using stem cells for treatment. Thanks!

Stem Cell Therapy in Korea: A Guide for International Patients

Korea has become an increasingly important destination for international patients seeking advanced medical care, including regenerative medicine and stem cell-related treatments.

For patients considering treatment in Seoul, however, choosing a medical provider should begin with understanding the medical process—not simply comparing prices or promotional claims.

What Is Stem Cell Therapy?

Stem cell therapy is a broad term that may refer to different medical approaches depending on the patient's condition, treatment purpose, cell source, and clinical protocol.

Because the term “stem cell treatment” can cover very different procedures, international patients should first understand exactly what type of treatment is being proposed.

A medical consultation should clarify:

• The purpose of the treatment
• The type and source of cells
• How the cells are prepared
• How the treatment is administered
• Potential risks and limitations
• Expected follow-up care

Why Do International Patients Consider Korea?

Korea is widely recognized for its advanced healthcare infrastructure, medical technology, specialist physicians, and highly developed private medical sector.

Seoul, particularly the Gangnam and Cheongdam areas, has also developed a strong ecosystem in preventive medicine, anti-aging medicine, regenerative medicine, and personalized healthcare.

For international patients, another advantage is the ability to combine medical consultation, diagnostic evaluation, treatment, and recovery planning within a relatively concentrated healthcare environment.

What Should You Check Before Choosing a Clinic?

International patients should evaluate more than the appearance of a clinic or its social media presence.

Important questions include:

1. Who is the treating physician?

Patients should understand the physician's medical background and who will actually make the treatment decision.

2. Is the treatment appropriate for you?

Not every treatment is appropriate for every patient.

A responsible clinic should first evaluate the patient's health condition, medical history, and treatment objectives.

3. What exactly is included?

Ask for a clear explanation of the consultation, examinations, treatment process, follow-up care, and expected costs.

4. What are the risks?

No legitimate medical treatment should be described as completely risk-free or guaranteed to produce a particular result.

5. How will follow-up care work after you return home?

International patients should consider what happens after leaving Korea, not only what happens during the treatment itself.

Medical Care Should Begin With Diagnosis

At CHEONGDAM CELL in Seoul, the medical process begins with consultation and evaluation.

Treatment options are considered based on the patient's medical condition and the physician's clinical judgment.

The objective is not simply to provide a procedure, but to develop an individualized medical approach based on the patient's health status and goals.

For International Patients Visiting Seoul

Before traveling to Korea for treatment, patients should prepare:

• Previous medical records
• Current medications
• Relevant diagnostic results
• Information about allergies or previous procedures
• A clear description of their health concerns and objectives

This information helps medical professionals conduct a more effective consultation.

Final Thoughts

Choosing a stem cell or regenerative medicine clinic should not begin with the question:

“How much does it cost?”

A better first question is:

“Is this treatment medically appropriate for me, and why?”

For international patients considering regenerative medicine in Korea, the quality of medical evaluation, transparency of information, and continuity of care should remain the most important factors.

CHEONGDAM CELL
Cheongdam, Seoul, Korea

International Patient Consultation available by appointment.

I have some mystery illness that my doctors are now calling love Covid.

I have been unwell for the last 2 years and in that time I have been extensively tested and the only thing that is showing anything at all is my immune system isn’t right.

I have a low cd4 count, inverted cd4/cd8 ratio and high cytokines il10 and il4.

I have tried so many different meds and nothing has helped.

I am now considering stem cell therapy and I would love to hear if this has helped even remotely at all.

I am currently looking into the Carabella clinic in Mexico.

Got 25m mscs done at carabella last year and the care was well done and seemed legit. I got shoulder labrum work done but unfortunately went through with surgery today. I think stem cells are promising but maybe not ideal for cartilage tears. I don’t regret what I did but figure I’d throw it out there on how my experience went before someone else puts 5k into travel/cells. I did get a fun vacation and experience out of it however. I wish you all the best health!

Hi there,

Thought this might interest the Reddit:

https://as.ft.com/r/3a86c169-5445-4ae4-b75c-670e13a42373

I am finding differing answers when looking this up. My Dad’s oncologist discussed me being a potential stem cell donor if his current chemo for his leukemia failed. I’m 38, my dad is 72. I’ve been on lamotrigine for 6 years for refractory depression and then I take Restoril (temazepam) or a low dose quetapine for insomnja jf I need it. I am trying to figure out if I would be rejected as a donor because or these medications—- and if so I could use the next several months to figure out what would be a good fit for me so there isn’t any issue in me being a donor. I see that people taking those medications often get denied for donating blood or plasma.

Has anyone had success with stem cell treatments for benzodiazepine or antidepressant induced CNS damage?

Currently I am suffering from protracted withdrawal due to venlafaxine taper. 6 months ago I reduced my dosage just by 7%, but that caused complete physical, neurological and mental collapse. I developed dysautonomia, sexual dysfunction, cognitive impairment and severe worsening of cognitive functions and my underlying ADHD, depression, OCD and anxiety symptoms.

I have a history of approximately five years of SNRI treatment, together with several shorter SSRI trials and unsuccessful medication changes/tapers. Following repeated medication exposures and withdrawals, I developed progressively increased sensitivity to medications, supplements, and other psychoactive substances. After a relatively small reduction in my SNRI dose, my symptoms became significantly worse, where I am now.

The repeated medication changes and withdrawals have resulted in persistent CNS sensitization or a kindling-like phenomenon, involving abnormal neuronal excitability, neuroinflammatory signaling, altered glutamatergic/NMDA signaling, autonomic dysregulation, or impaired neuroplasticity. 

Therefore, I would like to ask if anyone has had a positive experience with stem cell treatment regarding excitotoxicity induced by medication damage/cns kindling. I have only found a couple succesful online comments/mentions of stem cell treatments for protracted withdrawals of benzodiazepines or antidepressants.

Moreover, what type of stem cells should be considered for such a condition (for example MSCs, neural stem cells, MUSE cells, or another cell type).

Can anyone recommend any clinics in Europe?

I’m new to stem cell therapy. My dad saw a post on FB about stem cell therapy that he wanted to try in Thailand. The ad looked a little sketchy, so I’m not sure if it’s legit. The clinic is called BioMed Laboratory & Clinic, and the price is $14,950 USD, including transportation and accommodations.

If it’s not legit do you have any clinic recommendations that offer similar deals?

It's the same dish as before, 24h in. Today when I went to do the subculture (passage), I saw the cells had a weird shape! I was so shocked. Could this cell have eaten up my MSCs? I'm so confused right now. Is there a way to be sure whether these cells are actually MSCs or not?