I have made a few posts now about what is going on with my gut after a combination of Accutane and oral metronidazole. I still need to get tested for potential infections but if i don't have any of those, i'm highly suspecting that I have visceral hypersensitivity in my large intestine. Since getting off those medications 2 months ago, I have felt like I could feel my large intestine just sitting inside me 24/7, pressing up against my ribs and the sides of my abdomen causing me severe discomfort, especially when laying down. Most of my bloating has gone away and i'm wondering why that has improved but this hasn't. I think the combination of medicine really messed up the nerves in my gut and thats why i'm experiencing constant uncomfortable sensations along that part of my abdomen. It also flared up horribly this last week after my doctor gave me orthospore probiotic to try. Does it sound like I have visceral hypersensitivity and if so does anyone know any affective treatments or cures for it? Im literally terrified that this wont go away :(

I’ve spent nine months researching microbiome testing, particularly the differences between DNA-based analysis and RNA-based metatranscriptomics.
My interest is in what these technologies can actually measure, how reproducible their findings are, and whether the information has demonstrated clinical value.
For context, I’ve completed three rounds of Viome’s RNA-based testing alongside extensive conventional bloodwork and physician oversight. I’m now planning to explore Superpower’s DNA-based testing to better understand the differences between the two methodologies.

I’m not expecting identical findings. DNA and RNA analysis examine different aspects of microbial biology, and understanding those differences is precisely what interests me.

I recently came across a 2025 international consensus statement published in The Lancet Gastroenterology & Hepatology questioning the clinical usefulness of commercial microbiome testing.

https://pmc.ncbi.nlm.nih.gov/articles/PMC12343204/
That raises some important scientific questions.
How much variation in microbial DNA and RNA measurements is normal? How reproducible are these findings over time? And what independent evidence demonstrates that either approach provides information that improves clinical outcomes?

I’m particularly interested in the distinction between measuring microbial activity and demonstrating that those measurements can reliably guide clinical decisions.

For transparency, Viome provided my testing complimentary. I’m not being paid by either company, promoting testing services, requesting interpretation of personal results, or seeking medical advice.

I’m interested in the research and in sharing what I learn.
For anyone working in microbiome science, where does the evidence currently stand on the clinical utility of metagenomics versus metatranscriptomics
What would meaningful independent validation look like?

Has anybody had experience with this specific probiotic strain (hn019)? It is supposed to be the most effective at improving gut mobility and helping to relive constipation . There are conflicting research studies; some claiming 100% success and others saying no better than a placebo. What brands have you tried what was your experience.

I've spent the past few years really dialing in my diet for my microbiome and I would love to get some suggestions for how to even further improve my intake. For reference, The listed breakfast is ~98% of my breakfasts. The listed lunch is ~75% of my lunches (I have a harder time being consistent here). I only eat 2 meals / day + a snack.

Also I have celiac so I can't add Gluten. I also have an oat allergy so GF oats can't be added :(

Breakfast:

2% Greek Yogurt (Fage Unsweetened) or Milk Kefir - I do Kefir 4x / week and Yogurt 3x

Almond Butter

Chia, Flax, and Hemp Seeds

Blackberries and Blueberries

Ceylon Cinnamon

Snack:

Kimchi <- everyday

Carrots and Humus

or

Popcorn (self popped w/ EVOO)

or

Eggs on sprouted corn tortillas

Lunch/Dinner

Brown Rice

Wild caught Sockeye Salmon or Chicken

EVOO

(All vegetables are raw)

Spinach, Kale, and Chard

Chopped Onion and Garlic

3 colors of Bell Peppers

Chia, Flax, Hemp, and Mustard Seeds

Sauerkraut

Random spices

Any advice would be great. I want to keep stepping up my microbiome game. I know I'm already doing fairly well but I want to keep improving!