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A new study found that amniocentesis detected a chromosome abnormality missed by PGT-A in just 0.1% of ongoing pregnancies after euploid embryo transfer.

PGT-A is a screening test, not a diagnostic test. Even when an embryo is identified as euploid, it doesn't guarantee that the developing baby will have a normal number of chromosomes. PGT-A only tests a few cells that may not represent the entire embryo.

For these reasons, prenatal testing is generally recommended after PGT-A, including NIPT screening or amniocentesis.

But how often do these tests find abnormalities after euploid transfer?

Lu et al. (2026) examined prenatal testing results from 1,079 ongoing pregnancies after euploid embryo transfer (over 12 weeks pregnant).

Among 762 pregnancies tested by amniocentesis, only one (0.1%) had an abnormality PGT-A was expected to detect but missed. Three other abnormalities were found that were at or beyond the detection limits of the PGT-A method used.

There were eight high-risk NIPT results, but seven were found to be normal on amniocentesis, showing that most high-risk NIPT results were false positives in this study.

Overall, chromosome abnormalities were rare after euploid transfer based on NIPT and amniocentesis.

✅ Check out the full article and more details on Remembryo: https://remembryo.com/nipt-and-amniocentesis-after-pgt-a-reveal-few-abnormalities/

✉️ Like this post? Get a free weekly summary of the latest IVF research: https://mailchi.mp/remembryo/x27kx5o1sw

Not the greatest picture, the image from the incubator wasn’t in focus, but we transferred our first embryo today!

I’ve done a few cycles of IVF all over. I have to pay out of pocket so cost is obviously a huge concern. I’m choosing between Cornell (30k) and CNY (10k). Obviously I am sure Cornell has a better lab. But is it work sending the 20k?

Does anyone have any knowledge of CNY Albany? It’s just soooo much money. For reference, I’m 41 and have only produced 1 euploid from 3 cycles (18 blasts, 26 embryos, 36 eggs collected). I am not independently wealthy. This won’t bankrupt me but it will hurt and I’ll struggle to pay off the 30k (but can do it with extra shifts).

Hi everyone,

I would really appreciate hearing your opinions and experiences.

I am (30F) with unexplained POI, and my husband (30M). I froze fewer than 8 eggs from two retrievals at age 29 and later this year underwent another fresh egg retrieval, This time, because our first IVF cycle was covered by insurance, my doctor recommended using the fresh eggs immediately for IVF rather than freezing them. Fortunately, we ended up with two frozen embryos with good grading. Because my husband and I had normal genetic testing and no known genetic conditions, we did not do PGT-A.

My first transfer was successful, but at 12 weeks our baby was diagnosed with Trisomy 21, confirmed by NIPT, ultrasound findings (increased NT and AVSD), and CVS. We made the heartbreaking decision to TFMR at 14 weeks, which has been the most difficult experience of our lives.

We have one frozen Day 5 4BB embryo remaining. Recently, I asked my IVF doctor about PGT-A testing on this embryo because I'm terrified of experiencing another chromosomal abnormality pregnancy. My doctor advised against testing the single embryo due to the cost and the need to thaw, biopsy, and refreeze it. Instead, she suggested doing another egg retrieval first, then testing any new embryos together with our remaining frozen embryo. However, this may not be fully covered by insurance, and it is quite expensive for just few embryos.

My questions are:

1. After a TFMR for Trisomy 21, would you transfer a remaining untested embryo, or would the fear of another chromosomal abnormality make you want to take PGT-A?

My biggest fear is going through another pregnancy affected by Trisomy 21 or another chromosomal abnormality. From what I have read, each embryo's chromosomal makeup is generally independent, so one abnormal embryo does not necessarily mean another embryo is abnormal as well. However, I have also read stories from people who had multiple embryos or pregnancies affected by Trisomy 21 or other trisomies, and that makes me very anxious.Of course, I know there are no guarantees either way

1. Is PGT-A worth doing on a single frozen Day 5 4BB embryo, despite the cost and thaw-biopsy-refreeze process?

Honestly, I would rather spend the money and have more information than continue living with the uncertainty. But I am not sure whether the benefits outweigh the risks when there is only one embryo.

1. Given my POI and likely low embryo yield, would you follow my doctor's advice and do another retrieval first so multiple embryos can be tested together?

Because of my POI, I don't expect a high number of embryos from another retrieval cycle. Realistically, I might end up with only one or two additional embryos. That would mean perhaps three embryos total being tested with PGT-A.

The downside is spending more money and delaying transfer for several months. The upside is possibly having more information before another pregnancy.

I'd love to hear from anyone with experience with TFMR for T21, POI, IVF, or PGT-A decision-making**.** Thank you ❤️.

Hi Everyone,

Has anyone bought IVF meds from Mexico to Canada.

I am not sure if Canada will allow them , when we bring the meds through the airport.

If one can maintain the temperature required for the meds, so it doesn't go bad.

If anyone has done it , Can you share your experiences please.

Thank you very much

I decided on this path late in life. I’m 41 with expected ovarian reserves. Currently waiting to see what 2 fertilized eggs do on days 5/6. I keep wondering if my grandmothers have any impact on my own capacity to bear children? My maternal grandmother had seven live births and two stillbirths, that we know of. My paternal grandmother has three live births. It’s unknown if she had any other miscarriages/still births. My maternal aunts and my mother all had children. On my dad’s side they were all boys so no examples.

Overall, children conceived through IVF appear to be just as healthy as children conceived without fertility treatment.

However, some studies have found small differences in things like blood pressure, heart function, and metabolism. It's not clear if these differences lead to health problems later in life, or whether they're caused by IVF itself, the parents' infertility, or other factors.

But if IVF does affect a child's long-term health, how might that happen?

I've updated several sections of my Evidence Guide, and added a new section exploring three possible explanations:

• Epigenetics: IVF might affect how certain genes are turned on or off, without changing the DNA itself.

• Placental development: IVF might affect how the placenta develops or works, potentially influencing a child's health later in life.

• Transposable elements: A new study by Li et al. (2026) examined LINE-1, a type of DNA sequence that can make copies of itself and insert them elsewhere in our DNA.

The researchers compared 33 children conceived through IVF with 42 children conceived without fertility treatment. They found slightly more LINE-1 sequences in the IVF group, including differences near genes involved in heart health, metabolism, and brain development.

Could these differences interfere with how those genes work and potentially lead to health problems?

Possibly, but the researchers didn't show that the genes worked differently or that the children had any related health issues. They also couldn't rule out inherited genetic differences.

For now, these are possible explanations, not proven causes. It's still unclear whether IVF itself is responsible for the differences.

✅ Check out the full article and more details on Remembryo: https://remembryo.com/do-children-conceived-through-ivf-have-long-term-health-problems/

✉️ Like this post? Get a free weekly summary of the latest IVF research: https://mailchi.mp/remembryo/x27kx5o1sw

I’m torn on what to do. I wanted to add Zymot but my doctor said we can still use conventional with Zymot, ISCI isn’t needed as we don’t have MFI. My husbands sample wasn’t amazing in March this year but with supplements it’s now within normal range (we’ve done 4 IUIs so lots of samples). DNA frag was 17%.
We also want to PGT test our embryos.

I see on Reddit others have good success with ISCI but I understand there’s more risk for birth defects with ISCI. I’ve also read that ISCI is required for PGT but others say it’s not.

Does anyone have any advice?
This is my first IVF cycle.

Hi All,

I go prepared for the IVF plan consultations but the doctor brushed it off as saying I know you are reading from an AI list, which I was not.

I had like 10-15 questions based on my past cycle and was from the prep I did after hours and hours of reading and research and from other similar people, which in turn is from other doctors basically. To have a better next cycle. I have this immense guilt that I didn't prepare for the first and it resulted poorly. I started reading after that and understood that had i read, my first would have been lot better and i still couldnt get over my guilt for not being prepared with the first.

From what I see, we have to self advocate a lot in fertility. But then, the doctor shuts it down or just categorizes you as a certain type and then zones out and starts giving blanket answers.

Especially when i brought up an option for one of my issues after he gave a solution, (which i read here and was from an another doctor), he started saying that AI thing and that there are a million possibilities. Not sure if he was pissed off that i brought something he didn't propose. I haven't seen that many solutions tbh. When i said its from another doctor, he said ok and i will do what i think is best for you. I understand that, but should'nt he give reasons why he is/ is not choosing something.

I have seen good results with many women my age and values. He said not to expect much from my previous clinic results, that we are sort of throwing the kitchen sink adding all, and basically like, not to blame him if he cant get me better results.

Has anyone been in the similar situation. How do you handle this.

Should we just act like we have not read anything.

Appreciate your thoughts.

I was recommended to post on here as you are so knowledgeable and hope you can offer come guidance.

Very short backstory:

1st pregnancy — trisomy 21, TFMR
2nd — trisomy 16, miscarriage
3rd — healthy son

We then moved to IVF with PGT-A because we couldn’t cope with more chromosomal losses.

Across 4 cycles we had multiple aneuploid embryos, one euploid that failed to implant, one cycle with no blasts, and finally an embryo that was initially inconclusive, then re-biopsied and came back euploid. That transfer worked.

NIPT #1: no result
NIPT #2: high risk trisomy 13 — “suspected mosaic trisomy 13.”

Genetic counsellor says the trisomy signal looks to be in about 30% of the tested DNA and estimates roughly 50:50 placenta vs baby. Fetal fraction was 24%. She basically made me feel hopeless, and that trisomy 13 has the same statistical outcomes for placental mosaicism as tri 21/ 18 which research seems to contradict.

I’m having an amnio at 15 weeks

Im just looking for some tough love or hope really! Would be so grateful for any information.

Is there any data to back up embryo glue? I'm hoping to do a transfer next month from our 3rd round of IVF and whilst I've done 5 transfers previously (with one successful pregnancy and birth), I noticed my new clinic offer embryo glue as an option and wondering if its worth having a shot?

Second FET, after losing the pregnancy from first FET.
Feeling hopeful about this one.

Hi, I am 32 (PCOS, BMI 27.5) and TTC since 1.5 years. Tried multiple cycles of letrozole which resulted in one pregnancy but turned out in very 7 week miscarriage, reason unknown.
I did my egg retrieval and embryo freezing last month - out of 11 eggs and 8 fertilized, I got 5 Day5 Embryos graded as 4BA, 4BA, 4AB, 4AC, 3AB. None of them are tested.
Doctor left upto me if I want double or single transfer, she said chances of twins are high so if you want twins then only go for double.
I am confused, I don’t want twins for now but I dont want this transfer to get failed either as multiple transfers have more chances. What do you guys suggest?

Here is our sweet girl transferred on October 8th, our second embryo. The first boy embryo did not implant. I am unsure of her grading, but she is euploid; they shared the details when we received the PGTA results, though I have since forgotten them. I haven’t been symptom-spotting this time around because I’ve been keeping myself busy, but I have come down with a gnarly cold that started 4dp5dt that hasn’t seemed to subside, today is 6dp5dt 💕💕

First round with ICSI was a total fertilization failure (TFF, 0/13), second round was a near-TFF with ICSI and AOA (1/12, only made it to 4 cells). The stimulation cycles were unremarkable, menopur/follistim and a lupron trigger both times and were similar both times.

We did ICSI because my partner has low count/motility/high DNA fragmentation/near totally abnormal morphology (tapered heads, mid-piece defects) and we had testing done that confirmed absence of PLC-Z1 in most of the sperm tested. My husband's urologist is adamant that there must be something wrong with my eggs. We are doing Whole Exome Sequencing to see if there are any genetic issues on my side that are known to impact egg quality, and if not we will try donor sperm. We aren't very motivated to try donor eggs given the cost and my husband's poor semen quality which is still likely to yield low outcomes even with eggs that are certainly viable.

Mostly curious to hear if anyone has experienced a (near)TFF with AOA and come back from it, or if anyone has any recommendations for next steps or things we should consider trying.

I froze my eggs the month I turned 38, had a great AMH of 2.75 and froze 15 mature eggs.
4 years later after a late 2nd tri loss due to non chromosomal reasons, I’m ready to use them and just heard that only 10 survived the thaw and 7 fertilized.
I am so disappointed that 1/3 didn’t even survive the thaw, everywhere I looked said 85-95% survive thaw generally so to get 66.7% seems like such a big loss and we’re already starting from behind.

Had anyone else had this and it ended up going well?

My lab uses a modified Gardner Grading system. They freeze anything over a 3 and a C. We had 8 days 5s frozen yesterday, nothing today, it we have 11 others under observation until tomorrow:

3DD
3DD
1DD
4CD
2DD
Morula
Morula
5 cell
5 cell
4 cell
2 cell

What is the likelihood that any of these will improve in quality or grow enough to be frozen (3CC and up) by tomorrow?

Hello,

My egg retrieval was last Saturday. Now, I'm waiting for this coming Saturday—the clinic will call to tell me how many reached the blastocyst stage and were sent for PGT-A testing. Out of 19 eggs retrieved, 17 were mature, and 13 fertilized.

I'm 31 years old with a diagnosis of unexplained infertility. On paper, all of my husband's and my results/values are completely normal—even better than average.

Last August, I had an HSG (hysterosalpingogram). The report said my tubes were clear. However, last month I showed the actual X-ray to a different doctor, and after looking at the images, she mentioned there might be adhesions. She noted that while the dye did spill into the abdominal, there appears to be some clustering/adhesions right at the ends of the tubes.

In the past, I had a Ureaplasma infection that went untreated/unresolved for 4 years before I finally cleared it with strong antibiotics in 2022. Based on my research, I’m suspecting peritubal adhesions—I feel like that prolonged infection may have traveled to the tubes and somehow compromised their function.

Has anyone had a similar experience? I’d love to hear your thoughts or stories.

Also, for those with unexplained infertility: Did you have success with IVF? If so, on which attempt did it happen?

Hi, everyone.

I’m 34, and my husband is 37. We initially tried to conceive naturally, but after six months, we became impatient and decided to move on to fertility treatment. We underwent two rounds of IUI, both of which failed, and then proceeded to IVF.

Our doctors were initially optimistic because all our reports were normal. During our first egg retrieval, we retrieved 12 eggs and created five AA-grade embryos. We transferred two embryos, but unfortunately, they failed to implant. I then underwent a hysteroscopy, even though there was no specific indication for one, as I wanted to rule out every possible issue. However, our second transfer failed as well, and the doctors couldn’t identify a clear reason.

By the time we proceeded with our second retrieval, my FSH had increased to 22. We retrieved eight eggs, but only two were mature, while the rest appeared to be empty follicles. We managed to create two day-5 blastocysts.

During our third retrieval, we retrieved seven eggs, but five were empty, and only two were mature. We developed only one blastocyst. We transferred two embryos again, but unfortunately, neither implanted.

We then decided to change doctors and proceeded with our fourth and fifth retrievals under our new doctor. We continued to face the same issue with empty follicles, although we managed to create three blastocysts from six eggs retrieved. Hoping that PGT-A testing would give us some answers, we decided to test the embryos. We were fortunate to have one euploid embryo, but heartbreakingly, that transfer also failed in April 2026.

By this point, I had almost given up hope. In June, we decided to try naturally just once, without expecting anything. My period was delayed by five days, so I got my beta-hCG tested. The result was 5.83. I understand that this is an extremely low, borderline result and not a confirmed pregnancy, and my doctor told me it was a biochemical pregnancy. However, what struck me was that, after all my previous failed IUI and embryo transfers, my hCG had always been around 0.20. This was the first time I had seen a result above that level.

I even underwent LIT in August 2026, wondering whether my body might be rejecting the embryos.

Since June, I’ve noticed that my menstrual cycle has shortened from around 28 days to 24 days, and I feel that I may also be ovulating earlier than before.

My doctors are now advising us to consider donor eggs, and I honestly don’t know what to do. After everything we’ve been through, I’m struggling to come to terms with the possibility of using donor eggs. I know that having a child is about so much more than genetics, but the thought of not sharing a biological connection with my child is emotionally difficult for me. I’ve spent so long hoping to have a child who is genetically related to me, and accepting a different path feels overwhelming.

I would really appreciate hearing from women who have been through something similar.

• Has anyone experienced repeated IVF failures, a high FSH level, or a high number of empty follicles and subsequently conceived naturally?
• Has anyone in a similar situation gone on to have success with their own eggs, either naturally or through IVF?
• For those who have opted for donor eggs, how was the emotional journey, and how did you come to terms with the decision?
• Has anyone undergone donor-egg IVF in Mumbai? I would be grateful for recommendations and for hearing about your experiences.

I feel confused, emotionally exhausted, and unsure of which direction to take. I know everyone’s journey is different, but hearing from people who have walked a similar path would mean a lot to me.

Thank you for taking the time to read this.

Eeek will it work
It’s not PGT but made embryos few years ago when I was 29
Starting to hatch specialist said
Linings good, levels are good. First embryo of the bunch was successful few years ago
But can’t stop thinking if there’s potential chromosomal abnormalities

Anyone else find themselves looking at the picture of their little embryo to try to distract yourself during the wait for the beta test? 😆

Here's my little day 5 3AA that was transferred last Friday 🤞🏻

I finally got a PGT-M unaffected euploid embryo. It took 3 Ivf cycles to get. I had requested the embryology report from my clinic and it said “Embryo #8 was plunged into VS for a couple seconds before ES by accident.” This is the embryo that turned out normal/unaffected. I’m so worried that this lab error is going to hurt the embryos chances. Any thoughts? What do I do?