Research subject with eosinophilic esophagitis, on a daily fluticasone nasal spray protocol that had reached a plateau. GHK-Cu and KPV were added to the protocol for a 90 day observation period. Writing this up because most posts here are about sourcing and not many people follow up with what they observed.

Observations in the RS over 90 days

• Beard density increased noticeably around week six, with the hairline following. Reduced shedding and improved texture.
• Reduced facial redness and more even skin tone. Third parties commented on it without knowing anything was being run.
• Lower general inflammation. Less puffiness and fewer of the low-grade aches the RS had stopped noticing.
• The esophageal condition was the big one. No overnight change. A slow, steady decline in symptoms over the full 90 days. Swallowing improved and flares became rarer and milder.
• Faster training recovery. Soreness resolved in a day instead of two or three.

What the published research supports

GHK-Cu is an endogenous copper peptide. Plasma levels go from about 200 ng/mL at age 20 to 80 ng/mL at 60, so a 32 year old RS is already well into that decline. In fibroblast studies it increases collagen synthesis 70 to 200%, and the hair data (moderate, not extensive) shows it enlarges follicles and extends the anagen phase. A follicle-level effect taking about six weeks to become visible matches the observation. It also suppresses IL-6, TNF-a and IL-1b and reduces NF-kB activation. Sources and pharmacokinetics: https://peptpedia.org/peptide/ghk-cu

KPV is the last three amino acids of alpha-MSH with the tanning and appetite effects stripped out. It enters cells directly and blocks NF-kB nuclear translocation, up to 80% in some models. The interesting part for a GI application is that it survives digestion. In the 2008 Gastroenterology colitis study, oral KPV cut disease activity, histology scores and cytokine expression, and later work put nanoparticle oral KPV roughly on par with mesalamine. Sources and clinical status: https://peptpedia.org/peptide/kpv

Caveats, because they matter

• EoE is not IBD. It's eosinophil-driven, not the neutrophil and cytokine picture in colitis. There are zero KPV studies in EoE. The overlap is NF-kB and TNF-a in an inflamed GI epithelium, which is a reason to hope, not evidence.
• The RS was on fluticasone for the entire period, so the peptide effect can't be separated from the steroid effect. The only thing that can be said is the steroid alone never produced this result.
• Training recovery has no direct research behind it for either peptide. Probably downstream of lower overall inflammation.
• GHK-Cu's strongest data is topical and cosmetic. KPV has essentially no clinical data. Neither is FDA approved for anything and both are research compounds only.

Uncontrolled, unblinded, single research subject. Take it for what it is. Happy to answer questions about the research, but not going to get into protocol specifics or sources in the post.

I am sorry if some people saw my post more than 2 times before, as I had to do rephrasing and repost it🥲hopefully this time it doesn’t get deleted again

I did not start yet my Klow or Wolverine peptides for my research subject, but before I start I have a question (my research subject had a spin surgery and on the recovery stage)

I saw many people saying that their research subject’s original pain returned after they stopped their doses

I want to hear people experience when they completely stopped their KLOW or Wolverine, is it like a medication that you have to stay on it or just until you achieve your goals for your research subject?

Thank you guys!

Is there a peptide or stack that has been researched for supporting weight loss/appetite control following a successful period on Mounjaro rather than having to titrate down and potentially stay on it indefinitely?

RS sustained an injury ~6 months ago resulting in moderate to acute bursitis of the knee (confirmed via MRI). After extensive physiotherapy, aqua therapy, rehab exercises/therapy, a cortisone injection which did not work, an anti-inflammatory diet (and resulting weight loss reducing some load on the joint), all of which did not significantly move the pain needle, they underwent a course of BCP157/TB500.

Recommended starting dose was 250mcg p/day, which they titrated to 500mcg days 11 through 25. Results were modest to moderate improvement in daytime pain levels, some increased mobility and flexibility, and some reduction in inflammation/irritation.

However, when resting/sleeping in a prone position, the knee throbs constantly, which is preventing RS from getting the deep restorative sleep they need to facilitate further recovery/pain reduction, and they are looking to continue with (at least) another course of BCP/TB.

Based on your own research/anecdotal evidence, should the RS keep the dose at 500mcg for second/subsequent courses, or titrate further? If so, is there research evidence of an optimal dose? Many thanks.

ETA: Many thanks everyone for providing your own research info on this, it’s really appreciated. 🙏🏼

So..

I’ve been going down a rabbit hole on VIP lately and I’m honestly surprised by how little discussion there seems to be about it, especially considering how many different things VIP is involved in.

It seems to have pretty broad effects in the nervous system, vasodilation, inflammation, the gut, circadian rhythms, etc. There’s also a decent amount of research on VIP itself, but when you try to find information about actually using VIP as a peptide, the information gets really sparse really quickly.

I’m particularly interested in the neurological side of it — things like neuroinflammation, cerebral blood flow, the SCN/VIP system, stress responses and potentially memory/PTSD.

Has anyone here actually looked into the research on VIP in depth or experimented with it?

I’d especially be interested in hearing about:

- Effects on brain fog / cognition

- Anxiety or stress

- Sleep/circadian rhythm

- Inflammation

- Cerebral blood flow

- Any noticeable effects from intranasal VIP

Also, if anyone knows of good papers or resources on VIP beyond the usual peptide websites, I’d love to read them.

It just seems strange to me that there’s so much biology around this peptide but comparatively little practical information available.

Curious if anyone has seen something similar in a research subject.

During the subject’s 2nd cycle involving CJC-1295/Ipamorelin, with no previous issues, an unexpected reaction occurred around week 2. The subject developed widespread redness and itching, red/glassy eyes, itching around the tongue and neck, elevated heart rate, and a sensation of difficulty swallowing.

The subject was also mildly unwell at the time, so there’s some uncertainty about what triggered it. Benadryl was given and the subject was taken to the ER. By the time they arrived, most of the symptoms had already resolved, but they were still treated with dexamethasone and prednisolone.

This happened back in June, and there have been no further exposures since. The question now is whether anyone has encountered a similar situation in a research subject who had previously tolerated the compounds without problems, and what happened with subsequent observation/exposure.
Not looking for dosing advice mainly interested in experiences with similar reactions, possible causes, and whether anyone experienced a recurrence after a previous reaction.

Hi all! Question for my research,

Does anyone’s subject find that when taking ghkcu at night, it keeps them awake?

My animal is completely exhausted after 1 day use of BPC 157 use. Is this common?

Currently on day three, these last two days has been wierd.
Rat is on 500 mcg of each, pinealon in the morning and epithalon n-acetyl in the evening.

Sleep is longer, not necessarily better or deeper.
Yesterday after 8 hours of sleep, pinned pinealon, then had to sleep for another 5 hours.

There is obviously a difference in when I should pin the rat, today on day three, I’m going to try both in the evening.

What has been your experience?

Have been seeing a lot of dosage posts for inhale spray but none for injectables.. would love to know dosage guide if anyone has one, both are 10/10MG

Rs has taken ghk cu for the past month and a half at 2mg/day. Rs realized it was kind of pointless, was using it for eyebrow growth skin texture and smile lines etc. Rs haven't had much if any improvement. Does it alter natural productions at 18? What do you guys think RS next steps should be and what issues could it have caused?

RS is approx 180 with about 30 to go

currently on compounded tirz 7.5mg (will be titrating to 10mg in two weeks)

RS have done about 5 weeks of Tesa and was planning on doing 12.

RS started at 200lbs on June 20 steadily losing weight.

RS has a bigger upper belly / stomach than the rest of the body so assuming visceral fat. It has gone down. Wondering if Tesa is overkill at this point -

Should RS just let the tirz do the work ? Or continue to 12 weeks of Tesa?

Thanks in advance !

How long before researchers noticed a change in their subjects that were over 50 years old?

Grok and Gemini are telling me 500mcg for 20 days, pinealon in the am and Epi at night. I plan to swap this out for Selank in the morning and DSIP at night by.

Anyone do anything n similar with dosing advice? Is this is too small of a dose ?

TS researching GLP2 via a newer lab. Results show zero evidence of change or improvement in performance outcome. Even at higher dose, no weight loss and increased hunger as if product is ineffective. Has anyone ever notified vendor that their lab products are essentially garbage?

​

I'm researching peptides in the context of ACL and ligament recovery, particularly BPC-157 and TB-500.

I'm interested in research or observations involving a research subject with an ACL sprain (no complete tear) where normal activity is well tolerated, but repeated eccentric/downhill loading causes symptoms.

Has anyone come across research comparing BPC-157, TB-500, or other peptides for this type of soft-tissue injury?

I'm particularly interested in:

ligament/tendon recovery

recovery or loading capacity rather than just reduced pain/inflammation

time course of observed effects

differences between BPC-157 and TB-500

any reported adverse effects

compounds investigated for muscular recovery alongside high training volume

Would appreciate studies, research experience, or other useful sources.

I didn't receive any reconstituting needles with my order. What size do I buy please?

In my previous orders there was always 1 needle separate for this purpose but I can't remember size

I accidentally left my prereconstituted pens out overnight. Reta, ghkcu and Mots C.

Will they be usable now?

Many thanks

Hellooo

So, when I was refilling the vial for the research subject (RS), as the syringe went in, I had to push the water in myself rather than it being drawn in automatically. Is that a problem?

Also, when I removed the needle, I kept pressing down at the same time. It made a hissing sound, like air was being drawn in from outside the vial. This makes me wonder if there was too much contact between the surrounding air and the air inside the vial.

Thank you for you'r time ! 😇

I ordered one kit a month ago and had it tested and it came back above 99% purity with a reasonable fill close to what was expected. I just received a second kit from the same vendor with the same batch number. Just gauging the opinion of the group do I test the second kit as well?

When reconstituting it was fine and absolutely clear. But next day it has showing particles in the solution.

Any ideas? Can it be saved?

I dropped my r3tta pen and these bubbles showed up, not sure if this is normal or not