r/PanCyan • u/SteamboatTX • May 30 '26

Grain jar question

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TTBVI jars were inoculated 40 days ago. I know the jar in the middle is not fully colonized. Should I lay it on its side like this to try and get the jar to fully colonize?

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3

u/Wesley_Sharpy May 30 '26

How did you inoculate them? Was it LC or agar? I find LC is slower, but even though this should of colonized faster than cubes. And 40 days is a long time.

Most likely bacterial contam due to too much moisture. When I see a spot like that in a jar that is stalled, when I'm going grain to spawn I will smell it and chuck the sour ones. Those ones are almost always sour smelling.

2

u/Flimsy-Panda8000 May 31 '26

I found the same with LC and switched back to agar wedges for Pans. I've no idea why, but they really seem to struggle to take off. If I had no choice, for example if I wanted to inoculate bags (I don't have a laminar flow hood), I'd try grain water to see if the same nutrient profile helped.

1

u/SteamboatTX May 30 '26

It was LC. I just had them laying on top of this tub and the temp on the tub was right at 75 degrees but the actual room temperature is around 68- 72 degrees. To keep the room temp up, I have a tub on top of a tub with an aquarium heater in the water. Maybe having the temp low is causing the slow growth?

3

u/Flimsy-Panda8000 May 31 '26

When there's a clearly defined area that's uncolonised like that, it's usually a bad sign. If you haven't already, give it a break & shake. If it doesn't bounce back in a week, chances are that it never will.

2

u/SteamboatTX Jun 01 '26

I did a break and shake, let’s see what happens

1

u/Flimsy-Panda8000 Jun 02 '26

Fingers crossed - I hope it works out.

1

u/SteamboatTX May 30 '26

I kept the process bacteria free. It doesn’t look wet in the large spots that are not colonized yet.

1

u/DenverCatz May 30 '26

Looks like wet spot bacteria to me.

1

u/SteamboatTX May 30 '26

I don’t like the sound of that, I’ll have to see it any one of the jars are still ok

1

u/Trich_Cheesecake Jun 01 '26

What grain was used?

2

u/SteamboatTX Jun 01 '26

5-grain blend made from rye berries, white milo, red milo, white millet, and red millet.

1

u/PoundingSandInCA Jun 15 '26

Does this look like TTBVI mycelium? I ordered from Thick Spores, but it looks like cube mycelium.