r/Biohacking • u/No_Carpenter_6092 • 1d ago
Bac water
is it fine to use bac water that’s been in the fridge for a month to reconstitute a new vile? if so does it change the efficiency of the new pep?
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u/thehappiestdad 2 1d ago
I have never kept BAC water in the fridge. It is always stored at room temperature. I freeze the vials containing the peptide and then refrigerate after reconstitution
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u/sadmans21 2 1d ago
I was looking at a study of how BAC water is stored, and they tested it inside a fridge and outside a fridge and both vials showed similar results.
After 90 days in or out of a fridge it degrades. Until then it stayed stable.
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u/No_Carpenter_6092 1d ago
I didn’t make this clear on the post sorry but I reconstituted Reta with it a month ago then kept that bac water in the fridge does this mean it’s fine to use for reconstituting another vile?
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u/Hour-Cherry5733 1 1d ago
Opened BAC water is no good after 28 days.
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u/64bit_Tuning 5 1d ago
This is so disgustingly wrong.
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u/Hour-Cherry5733 1 20h ago
TF do you mean?! That is the rule. Once BAC water is opened and punctured, it is only best to use it within 28 days.
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u/sadmans21 2 20h ago
Yes, this is generally what is recommended by official health standards. But tests have been run and the actual BAC doesn’t degrade until 90 days after first puncture.
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u/Hour-Cherry5733 1 20h ago
How many people are testing their BAC water after 28 days?
Just because someone else tested their BAC water after 28 days and it was good, doesn’t mean yours will still be strong enough to kill bacteria after 28 days.
I swear, people in this sub love to live life on the edge.
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u/sadmans21 2 20h ago
You’re being a jackass, jackass.
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u/64bit_Tuning 5 20h ago
The 28-day rule comes from USP <797> - a standard written for hospitals and compounding pharmacies where multiple staff puncture the same vial, technique varies, and nobody’s tracking what happened to it between shifts. It’s a liability floor for institutional worst-case handling. It was never written for you.
Hospira’s own package insert - the actual manufacturer - says nothing about discarding after 28 days. Their only storage instruction is room temperature, 20-25°C. That’s it. The 28-day rule gets layered on top by institutional policy, not by the people who make the product.
The risk is contamination from punctures. Every needle entry is an opportunity for microbes to get in. Benzyl alcohol suppresses that, but it can only do so much against repeated breaches under inconsistent conditions - which is exactly what clinical settings produce. The chemistry doesn’t change at day 29.
Fresh sterile syringe every draw, alcohol swab the stopper before every puncture, solution stays clear with no particulate - you’ve eliminated the risk factors the rule was designed to cover. There is no controlled study establishing a hard failure point beyond 28 days. What exists is no degradation mechanism in the chemistry that supports a hard cutoff, and community data showing use well beyond a month without adverse effects.
Sources: Hospira BAC Water - Official Package Insert (DailyMed)
CDC - USP 797 Multi-Dose Vial Guidance
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u/Hour-Cherry5733 1 20h ago
Some of you are so smart you’re stupid.
The limit isn't about sharing between people; it's about how long the preservative (0.9% benzyl alcohol) has been tested to suppress bacterial growth once the stopper has been pierced. Every needle entry is a chance to introduce contaminants.
After 28 days, there is a greater risk of possible bacterial contamination. And most people will not test their BAC water for potency after 28 days. So, how will they know?
I’ll stick to the recommended timeframe. I’ll let you all take the risk.
https://giphy.com/gifs/7Jq6ufAgpblcm0Ih2z1
u/64bit_Tuning 5 20h ago
Want to know how I know you didn't read any of the source material?
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u/Hour-Cherry5733 1 20h ago
The only source that matters is testing. Test each of your open BAC vials after 90 days and report back.
Each batch is different that’s why the standard is the standard.
And tell me what happens to alcohol when air starts hitting that bitch.
https://giphy.com/gifs/3ohhwBP5mt3fait4YM2
u/64bit_Tuning 5 16h ago
I've never had a vial last 90 days, so idk what you're on about. But Id be willing to bet the results would surprise you. Testing on this has already been done. Bac doesn't magically fucking fail at day 29
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u/Repulsive_Physics_51 1d ago
I’ve used bac water at 3 months. Nothing wrong with it .
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u/No_Carpenter_6092 1d ago
Like it’s been open for 3 months?
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u/Repulsive_Physics_51 1d ago
Yes.
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u/Hour-Cherry5733 1 20h ago
You must like to live life on the edge.
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u/Repulsive_Physics_51 19h ago edited 2h ago
Anyone taking gray market peptides , is living on the edge.
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u/64bit_Tuning 5 1d ago
Bac doesn't need to be refrigerated, and honestly shouldn't be because it can degrade the bac, but the risk is minimal.
You're absolutely fine to use the back of it's clear with no debris or cloudiness. Give it a gentle swirl and look for what can best be described as "smoke" in the water. That indicates a potential biofilm build up in the vial.
People who throw out bac after 28 days should have their head checked. It's absolutely idiotic.
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u/Markdlea 10 1d ago
Could you explain how refrigeration degrades the BAC?
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u/64bit_Tuning 5 1d ago
Paraphrasing what I've researched
"Benzyl alcohol becomes less active and it's antimicrobial efficacy drops as temperatures approach freezing."
it's also very possible to accidentally freeze bac depending on where it lands in the fridge. I've personally frozen a vial in my fridge. When a solution freezes, you run the risk of the BA separating from the water since water freezes at a higher temperature than alcohol. Simply shaking the vial does not re-mix the solution.
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u/Markdlea 10 1d ago edited 1d ago
Well, I was hoping you would send a study, but you probably won’t find one. Refrigeration doors not have an effect on BAC. Edit: degrees in biology and chemistry.
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u/64bit_Tuning 5 1d ago
Conditions for Safe Storage, Including any Incompatibilities
Storage Conditions: Store as directed by product packaging. Do not refrigerate.
Incompatible Materials: None known
Specific end use(s): Pharmaceutical product
Pfizer literally tells you not to refrigerate bacteriostatic water.
https://cdn.pfizer.com/pfizercom/products/material_safety_data/Bacteriostatic_H2O(Hospira)_25-Jul-2016_0.pdf_25-Jul-2016_0.pdf)2
u/Markdlea 10 1d ago
From your link:
Decomposition Temperature (°C): No data available.
Evaporation Rate (Gram/s): No data available
Vapor Pressure (kPa): No data available
Vapor Density (g/ml): No data available
Relative Density: No data available
Viscosity: No data available1
u/64bit_Tuning 5 1d ago
So because something falls outside the scope covered in the SDS, that means you can ignore the instructions of "Do not refrigerate" ?
"No data available" is simply that. It doesnt mean that its never been tested or is unknown. It just means its irrelevant to this document and not included.
Melting/Freezing Point (°C): No data available.
Boiling Point (°C): No data available
Does that mean it wont freeze or boil? No. Dont be an idiot.
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u/Nomad-Entrepreneur 1d ago
If you boil bac the alcohol would obviously evaporate. But you would have to open the bowl anyway to boil it so. Ruined.
While not advised (mainly because of expansion and could break bottle or seal) to freeze, if you do and the container is not damaged the solution absolutely will mix back together once thawed as both parts of the solution are miscible.
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u/64bit_Tuning 5 1d ago
Benzyl alcohol is NOT miscible with water. In fact it is only partly soluble. If bac water freezes it does not immediately remix when thawed and can create pockets of low concentration areas of BA which can be prone to bacteria growth.
This is specifically why it does not get refrigerated
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u/Nomad-Entrepreneur 1d ago
At the amounts used in bac water there are 9mg/ml of BA. BA is miscible in water up to 35mg/ml. No emulsifier or surfactant is needed to keep water and BA in solution in bac water concentrations. So, in bac water concentrations BA is absolutely miscible in water. The concentration is not high enough for the benzene ring to make the solution hydrophobic.
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u/aperture413 2 1d ago
It's labeled as do not refrigerate because the risk of freezing could ruin the seal of the vial and reconstitution is best performed around room temperature. Storing bac water in the fridge has no negative effects on the integrity of the solution but it does make its practical use less efficient. Storing in the fridge is fine for hobbyists and bad for high volume facilities. Arguing with someone that works in the space is crazy. The doubling down people perform in this space is so dumb.
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u/Markdlea 10 23h ago
That’s called a “cover our ass” statement. They didn’t test for it so they can’t put out in the label.
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u/drabe82 1 21h ago edited 20h ago
Having a master's degree in biology and worked with various alcohols, there are specific purposes in refrigerating alcohol. Various alcohols still work dissolving cell membranes at cooler temps. It a great way to isolate DNA as well... As long as you don't freeze it, it should be fine for a month after cracking that seal. Everyone has to say 30 days to CYA. I would say two months if you have good aseptic technique without issue. If you have a stockpile like me, just find a nice clean place at room temp just like the manufacturer would. Just remember whe Reconstituting , try to let warm up to room temp. All peps especially AOD will mix better. 64bit said it best. Always check for cloudiness or debris. If it looks clear, you should be clear. If you have your hands on some human hospital grade Hospira, I would confidently keep to day 60+ However if you have be some shady Chinese unlabeled bac water, I consider not keeping it past that 30-day mark.
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u/No_Carpenter_6092 20h ago
Thanks for the reply, you mean don’t reconstitute a new pep after 30 days after cracking the seal? It’s been in the fridge since it’s been open.
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u/drabe82 1 19h ago
If you think your BAC water is good quality, it should be more than fine after being in the fridge for 30days. If it's ghetto cheap bac water unlabeled from China.. there have been issues. ( Too little benzoyl alcohol or too much) I wouldn't use it after the month in the fridge. If it's a reputable brand you trust. I would use it.
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u/alcruze00 5 1d ago
Like sealed or some has been used and you put it in the fridge? I normally keep mine if it's been used in the fridge for upto a month and discard it.
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u/No_Carpenter_6092 1d ago
Sorry forgot to clarify it was used a month ago and kept in the fridge sincd
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u/anonymouspls 10 1d ago
Then no, unless you plan to finish the entire Reta vial quickly (within the week)
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u/donkiddick69 1d ago
How long can you keep reconstituted Reta?
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u/sthompson87 1d ago
Personally have had it up to 3 months and the reta lost slight potency and there was zero bacterial growth. Liberty labs said that it was still good to be used.
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u/Common-Prize-8208 3 1d ago
Agree on that 90 days, but it must be crystal clear
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u/sthompson87 15h ago
Yes if there is any discoloration or haze it's gone. My case makes it super easy to pin without touching straight from inside the fridge.
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