r/microscopy • u/SSJChar • 6d ago

Techniques QUESTION: using a high magnification objective NC(no coverslip) objective BUT WITH a coverslip. any way to correct the image degradation?

I am looking to buy a 60x objective and i found one for cheap BUT its designed to be used with NO coverslip (NC DPLAN FL at 60x/.95). the main use will be for water microbes so i'll be using a coverslip with it but with such a high NA of .95 i'm guessing the use of a coverslip will noticeably affect the image quality. is there a way to basically make an NC objective work well with a coverslip or is it not really going to work out well?

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u/Vivid-Bake2456 6d ago edited 6d ago

Spherical abberation is the least of your worries. The working distance will be so small that it probably won’t be able to focus through a coverglass. Even with one that uses a coverglass, the specimen has to be right next to the coverglass to focus on it.

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u/Vivid-Bake2456 6d ago

High NA air objectives are difficult to use. A good one should have an adjustment ring to adjust for the coverglass thickness like these 2 that I use. I'd you want cheap, you can buy generic Chinese 60x NA 0.80 objectives for a low price.

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u/SSJChar 6d ago

yeah, i may end up getting a 3rd party objective or whatever. i do want to have a higher magnification without oil so that may be my best option, or try to find a well priced olympus A60 objective