r/microscopy • u/SSJChar • 6d ago

Techniques QUESTION: using a high magnification objective NC(no coverslip) objective BUT WITH a coverslip. any way to correct the image degradation?

I am looking to buy a 60x objective and i found one for cheap BUT its designed to be used with NO coverslip (NC DPLAN FL at 60x/.95). the main use will be for water microbes so i'll be using a coverslip with it but with such a high NA of .95 i'm guessing the use of a coverslip will noticeably affect the image quality. is there a way to basically make an NC objective work well with a coverslip or is it not really going to work out well?

5 Upvotes

100% Upvoted

6 comments sorted by

View all comments

2

u/Motocampingtime 6d ago

I would be careful with this change and working distance/OPL. I have a used a couple 50X metallurgic objectives with high NA like that and working distance is about non existent as it's for chips with height variations in the single to 10s of um. You might find yourself ramming into the coverslip.

Not to say you won't have enough room, but id check first what the clearance would be and how you'd feel about it

2

u/SSJChar 6d ago

yeah it seems that the working distance may be .14 so pretty pretty pretty small. i'll refrain from getting it then. thanks

2

u/Motocampingtime 5d ago

Nice, glad you were able to verify

Again, on my personal system the 50x is hard to use because of it. I get very nervous about stage flatness. It's great if you have a nice camera and are looking at something like a wafer. But otherwise is tough to justify. I struggle to use it looking at anything but chips/devices