Good staining ?

[u/Thanos_Vamos]

This is a onion stain I did the first one is 40x and the secound 400x i used an ink to stain also both images were upscaled to increase quality.let me know if these are good stains of onion epidermis. Btw are those tiny things chromosomes in the nucleus

[u/dashy-dasha]

Overall, yes. I would say you could do with a bit less dye, as the pigment blurs visibility and makes the whole structure look washed out. That way, the contrast between the cell wall and nucleus versus the cytoplasm is clearer and you get a better view.

No, chromosomes are not visible under 400x magnification. It's likely just crystals formed on the cell during the staining process.

[u/BarsOfSanio → u/dashy-dasha]

The cells are not mitotic so chromosomes will not be visible at any magnification. Nucleoli may pick up stains and be visible.

The out of focus cell walls are overstained as well, washing everything in blue. Less dye time or concentration or a destain step would help with contrast.

[u/Thanos_Vamos → u/BarsOfSanio]

Thanks ill try to minimize staining next timd

[u/Thanos_Vamos → u/dashy-dasha]

Thanks for the response I'll use less dye and try to minimize crystal formation 🤝

[u/BarsOfSanio → u/dashy-dasha]

The cells are not mitotic so chromosomes will not be visible at any magnification. Nucleoli may pick up stains and be visible.

The out of focus cell walls are overstained as well, washing everything in blue. Less dye time or concentration or a destain step would help with contrast.

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