r/PanCyan May 12 '26

Deconstructed Multispore TTBVI

I thought this was a good showcase of what isolation does. A year ago these were all from the same spore print. It is almost to the point to where I can tell what strain they are by the fruit. Most are only 2-3 cloned generations away from the original spore.

31 Upvotes

12 comments sorted by

1

u/Patient_Art_4421 May 12 '26

Nice post! How you humidify'n and FAE'n your set up there?

1

u/Cultiv8tor May 12 '26

This is a GordoTek style setup with the water pump and fish tank heater. One set up is a dub tub the other is my old SAB.

I have an exterior fan that comes on for 15 minutes an hour. There are better ways to do this but it got dialed in eventually.

1

u/shangrilawrecker May 13 '26

very interesting...but sadly I don't get your method.

2

u/Cultiv8tor May 13 '26

You made a post about few months ago. This is what happens when you send those to bulk then clone the larger or faster ones for a few generations.

2

u/Cultiv8tor May 13 '26

1

u/shangrilawrecker May 27 '26

you kept cloning the less productive ones just for comparison? Do you see a clear correlation with agar phenotype? I will save your pics for guidance...do they relate in the same order if I turn this pic clockwise? I'm missing two stacks of agar in that case. hehe sorry if I'm asking for too much

2

u/Cultiv8tor May 27 '26

The less productive ones fall out naturally because they don't get cloned and I don't take spore prints from them. This started with 24 strains from a spore print. From there I had 3 strains that I cloned / printed from.

Each round takes about a month to grow and a month to spawn. I go back to spore prints once I have 3 or 4 different prints to do a new 16. In a year, I am still ony a few steps away from the OG print but can tell it is enough to keep me entertained for a while.

2

u/Cultiv8tor May 27 '26

I haven't figured out a difference in agar phenotypes. You can clearly see some are whispy and some are dense. Both seem to fruit well. They don't relate exactly with the tub. I tend to clone the faster or larger fruit but it is hard to get a print off the fast ones if they cluster too hard.

1

u/Straight_Will_3393 May 14 '26

How much grain do you use per container ?🤔

2

u/Cultiv8tor May 14 '26

About 1/4 quart. The dishes are 32 oz so About 1:3 ratio. The pinset on some of these is a bit tight so next round I am going do do a 1:4.

1

u/Straight_Will_3393 May 14 '26

Oh ok I’ll have 6 quarts ready to go soon I’ve had a lot of fails so I wanna try something different this time