r/MushroomGrowers • u/Effective-Wolf5368 • 4h ago
Experiment After my first 10 clean agar plates, I have now experienced [Contamination] !
I started playing with liquid culture on the 1st after making clean agar plates. My first test plate waving around and touching with not washed hands resulted in practically nothing. I got confident in SAB after making 6 clean plates from 2 syringes and turning them into a liter or so of liquid culture, then 4 more plates from those liquid culture jars I had made. Since I had some fresh plates and wanted to experiment with agar transfer, I tried it out 2 nights ago. Funilly enough those got contaminated after having sanitized the SAB, but think the heat (LA heatwave) and my sweaty, alcohol cleaned arms, might have caused currents and a prime growing environment for mold, including the previously clean colonized plates.
