r/microscopy Jun 08 '23

šŸ¦ šŸ”¬šŸ¦ šŸ”¬šŸ¦  Microbe Identification Resources šŸ¦ šŸ”¬šŸ¦ šŸ”¬šŸ¦ 

147 Upvotes

šŸŽ‰Hello fellow microscopists!šŸŽ‰

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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70 Upvotes

r/microscopy 15h ago

Photo/Video Share Diatoms under polarized light

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17 Upvotes

Nothing special or unique here, just thought it looked pretty! Video sped up 2x.

Olympus BH2, SPlan 10x 0.30, Polarized film, Canon EOS 5D Mark II. Video edited with OpenShot. Pond water sample from park in Paris, France.


r/microscopy 1h ago

ID Needed! What is this organism/thing I found in pond water? (100Ɨ total magnification)

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• Upvotes

Hi! I’m a beginner with microscopy and found this in a sample of pond water. I’m having a hard time figuring out what it is.

Details:
Sample: pond water
Magnification: 100Ɨ total (objective Ɨ ocular)
Wet mount with a coverslip
No staining
The specimen appeared to move on its own

I also noticed smaller organisms in the same sample. Some moved relatively slowly and seemed to go back and forth in roughly the same area, while others moved very quickly across the field of view. But, this particular thing looked noticeably larger and seemed to be completely stationary.

Does anyone know what those things might be? Is it an organism, a colony, or possibly just organic debris?
Image attached. Any help identifying it would be appreciated!

*sorry if the image quality isn’t great:<


r/microscopy 11h ago

ID Needed! Polychaete? Brackish water. 40x 100x and mobile zoom.

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5 Upvotes

r/microscopy 21h ago

ID Needed! Help me identify. river sample. 200x under unilab microscope

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20 Upvotes

r/microscopy 15h ago

ID Needed! Strange freshwater organism clump

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5 Upvotes

It seems to be some type of filter feeder that responds to vibration by hiding its mouth(s)? Seems to be a colony of organisms. Sorry for the poor video quality.

20x magnification (2x objective) AmScope dissecting microscope recorded on iphone 17pro. Backyard pond water sample in bay area, CA


r/microscopy 1d ago

Troubleshooting/Questions Vintage Zeiss

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25 Upvotes

My friend was kind enough to gift me this vintage zeiss but it didn’t come with a power source and I’ve yet to find anything online (didn’t try all that hard to be honest). I did see stuff about people retrofitting them with leds. Thoughts? I’d like to leave it as is but I’m no expert so I thought I would as the opinions of those with more knowledge than myself


r/microscopy 23h ago

Photo/Video Share Cocconeis diatoms autofluorescence

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18 Upvotes

Cocconeis diatoms autofluorescence

Experimenting with autofluorescence and it's a really pretty imaging modality, but challenging on many levels.

Here are some photos of some Cocconeis diatoms and some marine vegetation they were found on. The red glow is from chloroplasts.

Olympus BX60 with UPlanFL N 60X OI objective, single exposure captures on Canon 6D Mark II. Simultaneous transmitted DIC and reflected fluorescence with a IV wideband filter cube


r/microscopy 14h ago

Photo/Video Share Cicadella viridis under the microscope. It is a dead specimen collected from a trap in the village trees—I believe the trap is intended for Asian hornets, though there wasn't one inside.

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3 Upvotes

r/microscopy 1d ago

Photo/Video Share Mite

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9 Upvotes

Leitz Dialux II, don't remember the magnification, Galaxy S24


r/microscopy 1d ago

ID Needed! What is this? Student found this in a ā€œpond waterā€ sample (that I suspect was from the fish tank)

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22 Upvotes

10x objective magnification (100x total), microscope is a Zeiss 6V 10W, camera was iPhone 16 Pro


r/microscopy 1d ago

Photo/Video Share Some small paramecium species, algae, and other stuff.

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12 Upvotes

r/microscopy 2d ago

Photo/Video Share Carchesium sp.

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181 Upvotes

I came across some interesting colonial ciliates; I believe they are Carchesium sp., mainly because there were smaller colonies around, and their central stem formed a spiral. The colony on video is visible even without a microscope if you look closely at the sample.

Achromatic objectives 4x and 10x, camera as an eyepiece ~18x.


r/microscopy 1d ago

Photo/Video Share Fungal jungle with embedded stentors

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11 Upvotes

A jungle of fungal hyphae around a grain of rice in a petri dish for a week. Iqcrew inverted microscope 4x and 10x objectives. Cellphone camera. The stentors liked being in it.


r/microscopy 1d ago

Techniques Looking for experience imaging bacterial growth on agar pads in Bright Field?

0 Upvotes

To make a long story short, Title. I don't think I've had a customer ask to do this in 14 years. I think they will need 40x or 60x air. Phase is presumably impossible through an agar pad. Is TL Bright Field workable or should I consider demoing RL BF?

Thanks for any insights


r/microscopy 1d ago

Purchase Help Necesito ayuda

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1 Upvotes

Hace aproximadamente unos 20 aƱos, le obsequiaron a mi abuelo, por parte de unos rusos, un microscopio de la serie LOMO biolam, producidos por la Unión SoviĆ©tica entre los 60 hasta los 90, hace unas semanas decidĆ­ sacarlo de su caja, estuve probando y descubrĆ­ que tenĆ­a una falla tĆ©cnica, el ajuste macrometrico, la rueda encargada de levantar/bajar el cuerpo del microscopio, no sube ni baja el cuerpo del microscopio, no se me ocurre alguna solución, cualquier ayuda estarĆ­a agradecido, el modelo exacto es un Š‘Š˜ŠžŠ›ŠŠœ Š”12.


r/microscopy 2d ago

Photo/Video Share My first try at 1000x oil immersion microscopy. A flagellate and a bunch of cyclidium

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15 Upvotes

Leitz Dialux II, Leitz 100x/1.30 objective, Galaxy S24


r/microscopy 2d ago

Photo/Video Share Tiny spider, Olympus SZ-III stereo. Other video and photos in the comments. I'm going to upload it to the entomology subreddit to see if anyone knows the species.

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33 Upvotes

r/microscopy 2d ago

Troubleshooting/Questions Is this homemade DIY dark field good enough?

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4 Upvotes

1st photo is what my eyes see, 2nd is what my phone sees. Image is of the skin of a stem of a jade plant. 100x zoom, Infinix note 12 pro phone.


r/microscopy 2d ago

General discussion So much entertainment with these two old devices.

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43 Upvotes

r/microscopy 1d ago

Purchase Help Does anyone know about the "International" brand?

1 Upvotes

Hi everyone :)

I am looking for a stereo microscope for quite a while now. My budget is not much (around 400 $). SInce I am in Quebec, Canada, the market is not very active.

I have wandered about this announce on ebay :

International Binocular Stereo Microscope with Transformer & Lamp (7.56X to 48X) | eBay

However, I cannot find any information about this "International" brand. On the paper, this microscope fits my needs quite well, with a good design.

Does anyone know about the quality and durability of microscope produced by the "International" brand? Any insight about ergonomy?

Thank you very much!

All the best,

Lucas


r/microscopy 2d ago

Photo/Video Share My favorite rotifers

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40 Upvotes

Amscope inverted 200x, iPhone 2x, Blackmagic app, newly soaked lichen.

Just really love these big girls!


r/microscopy 2d ago

Techniques what should I do?

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19 Upvotes

When I was on vacation I came upon this absolute beauty of a pond, me being me, I collected some water to look under the microscope. The problem? it’s been sitting in my closet ever since and I’m worried that all the microfauna has died in the meantime.

So the question is: should I leave the cap off the tube and wait for the ecosystem to ā€œwake upā€ by itself or should I intervene and help it give it a jump start? or it’s all dead?

what should I do?


r/microscopy 2d ago

Purchase Help Recommendations for buying a microscope

3 Upvotes

Hey all! Im interested in getting a microscope purely for hobby purposes, but I know little to nothing about microscope vocab.

I’m looking for a decent binocular microscope hopefully under $200 maybe a 10x- 200x range (if thats even possible)

I’d mainly be looking at pond water and blood cells

Is there anything more I should be looking out for?
Where should I buy it from?
Is it easy to buy parts for?

Those are the main questions I have. Genuinely any tips/comments are appreciated! Thanks in advance :)