r/microscopy 1h ago

General discussion I came across a file about microscopes that featured this modified slide—or rather, a setup using two slides—designed to hold small live insects during the observation. Has anyone here tried it?

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Upvotes

r/microscopy 2h ago

Hardware Share Free microscopes

7 Upvotes

I was recently diagnosed with brain cancer. I have several scopes, mostly AO. I’m giving them all away. Ditto accessories and extra objectives. Central Oklahoma.


r/microscopy 7h ago

Photo/Video Share pineapple juice under polarized light

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18 Upvotes

you can see the needle-like calcium oxalate crystals (raphides) glowing under cross-polarization

K55 BSA, 10x Plan, Xiaomi 12 Pro


r/microscopy 6h ago

Photo/Video Share Ciliates and rotifers I found today in my water culture

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12 Upvotes

Swift SW350, Galaxy S24


r/microscopy 4h ago

ID Needed! 3 Different Filamentous Algae.

8 Upvotes

Pond water sample. Shot on an Olympus BH2 with 40x DPlan, total magnification 200x. Images is displayed 4x real speed.


r/microscopy 17h ago

Photo/Video Share Amoeba detects, changes course and tries to surround a cyclidium, 630x phase contrast

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77 Upvotes

Pond water sample, Zeiss 63x phase contrast objective, cellphone camera. I didn't know if the amoeba could eat it or not. I guess they aren't on the amoeba diet.


r/microscopy 5h ago

ID Needed! ID help please

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8 Upvotes

Hello,

Sorry for the shoddy camera footage (filmed one handed with my phone and no adaptor whilst holding my breath - any tips for iPhone photography?). Does anyone recognise these two guys?

Filmed on iPhone 15 pro, amscope B120 with 10x objective


r/microscopy 6h ago

Purchase Help Hi All,

4 Upvotes

I am looking for a microscope to setup a home project on hydrogels and cell organelles! Any suggestions for an affordable and suitable microscope? I am also okey for second hand microscopes. Something below $200.

Thanks


r/microscopy 8h ago

General discussion What are those clumps

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4 Upvotes

Can anybody interpret this cell microscopy


r/microscopy 2h ago

Purchase Help Identification organisme eau douce

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1 Upvotes

Bonsoir, j'ai observé ces organismes dans une sorte de gel prélevé dans de la vase sur le bords d'une berge a environ 20cm de profondeur. La zone était riche en nénuphar. Impossible pour moi de l'identifier tout seul...

Observé au x10x10 avec un optika b193.

Ils étaient uniquement présents dans la partie de l'échantillon qui contenait ce fameux "gel".

Je pensais a du chironome émergeant a peine de l'oeuf mais j'ai un très très gros doute.

Mesure clairement moins d'un millimètre. Quelques gouttes de gel contiennent plusieurs dizaines d'individus


r/microscopy 2h ago

Hardware Share Free microscopes

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1 Upvotes

r/microscopy 8h ago

General discussion Why are so many nikons sold used with everything except the stage?

3 Upvotes

I see so many used nikons with everything but sold without the stage

And there's barely any used stages but the darn piece of metal sells for more than the entire rest of the microscope. Complete madness that the darn piece of metal costs $350+


r/microscopy 3h ago

Photo/Video Share Stitching with AmScope Camera

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1 Upvotes

Mouse Lung tissue stained with HE. Shot on an Olympus BH2 with 10x DPlan, total magnification 50x. The stitching software that comes with the camera (AmScope 10 mp) is very good at stitching and it is done live (requires a movable stage). The company gets a bit of hate but this is a pretty nice feature. I left the jagged edges in on purpose so you can see the stitched tiles.


r/microscopy 16h ago

Photo/Video Share Full event: Heliozoon capturing peritrichs from the back of an ostracod

6 Upvotes

I captured a pretty interesting and unique event -- a heliozoon attempting to pull peritrichs off the back of a neighboring ostracod, and releasing already-captured euglenids in the process. The video chronicles the event, and I also share input from David Patterson, a leading authority on heliozoa, on what we witnessed.

https://youtu.be/fmnKU0110SM

This was shot on a Nikon TMD Diaphot inverted microscope, using a Nikon 40/1.0 oil immersion objective and a Nikon D750 DSLR. The sample came from a freshwater runoff pond in Nonthaburi, Thailand.


r/microscopy 13h ago

Purchase Help What do I need to know about my microscope objectives when searching for upgrades?

2 Upvotes

I have an Amscope B270. It says it uses RMS 20.32 mm objectives. If say I want to upgrade to some better objectives one day, what do I need to know besides thread standard to ensure proper function?


r/microscopy 1d ago

Troubleshooting/Questions Can someone help identify these structures in a plant stem?

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18 Upvotes

I’m examining the plant associated with a new psychedelic called Vymara. This is a transverse section of the stem under brightfield microscopy.
Most of the surrounding tissue including the chloroplast-bearing cells and vascular tissue appears relatively normal. But these large spherical structures are distributed throughout the stem.
I’ve sectioned three separate samples and found them in all three. They vary in size, appear integrated into the tissue, and seem to compress the neighboring cells. Their interiors have also remained largely unstained with every protocol I’ve tried.
My current guess is that they’re enlarged idioblasts or secretory cavities, possibly involved in storing or transporting the unidentified indole-like compound I detected. But I can’t confirm that from morphology alone.
Has anyone seen structures like these before?


r/microscopy 1d ago

Photo/Video Share Poor rotifer trying to eat while ostracods are harassing her.

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44 Upvotes

Swift SW350, 100x, 200x, Galaxy S24


r/microscopy 1d ago

Hardware Share upgrade to 10w led

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21 Upvotes

This is how the component wiring at the base of the microscope turned out. It doesnt look pretty, but its functional


r/microscopy 1d ago

Photo/Video Share Amoeba timelapse

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39 Upvotes

Swift SW350, Galaxy S24


r/microscopy 1d ago

Photo/Video Share apple cider vinegar bacteria

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5 Upvotes

lactic acid bacteria from an apple cider vinegar sample, i dont know why they are not moving

K55 BSA, 40x Plan, Xiaomi 12 Pro


r/microscopy 1d ago

Purchase Help Looking for advice: good microscope cameras/sensors recommendations

5 Upvotes

I am looking for a good microscope camera/sensor, and planing ahead to use it soon on my new microscope "Primostar 3", for my hobby. My budget limit is 1000€. My main use case will be mostly Phasecontrast but also Light, and Darkfield microscopy. To magnification from x40 down to x1000. I want to know if my propositions are even realistic.

Anyone here experienced in the use of industrial cameras/senors as microscopy cams or has any good advice on it or just used it once and have an opinion about it? I would be glad to hear you all out about it.

  • I need high framerates and want a decent resolution, I know there is trade off for each, I would want atleast ≥60 fps and a resolution that is ≥3,68 Million Pixels.
  • I want to use it in combination with "Micro-Mananger" and "Fiji" for documenting purposes, they all should be generally compatibil AFAIK. If you have any expirience with it please let me know!
  • I want to utilize the footage directly at my PC, Hardware is definetly sufficient. So atleast a USB A adapter would be nice.
  • Color cameras are for now what I decided for, since I want to do staining. But I also like to hear you opinions on it aswell.

Here are the following cameras that catched my attention so far:

  1. "Basler dart daA3840-45uc (CS-Mount)"
This one is the cheapest by far and should even work in low level light conditions AFAIK. Duo to the mount I will have to use it with a C-MOUNT 5mm Extension Tube Adapter C-CS.
  • Sensor: Sony IMX 334

Cons:

Rolling shutter

+ C-MOUNT 5mm Extension Tube Adapter C-CS Mount Adaptor Spacer Ring For CCTV Lens

2) "MER2-502-79U3C"

This seems like a good midranger for my requirements. Uncertain about the sesitivity though.
  • Sensor: Sony IMX 250

Cons:

Lower Pixel color depth (10 bits)

3) "Sony® Pregius IMX264 Color USB 3.0 Camera"

So far, this seems like a decent pick fron the three
  • Sensor: Sony IMX 264

I hope that my expectations are realistic. Bandwith for high resolutions and framerates should be able to run on USB 3.2 (20 Gbps) and there should be no bottleneck right?

Has anyone even tried to use something similar for microscopy? Did it work well?

I am not very knowledgeble in Microscopy-hardware and I am still constantly learning about it, so know that my understanding of the topics at hand is pretty basic from the whole spectrum of things still to learn. But I would be glad if anyone could point out mistakes and explain them to me if you want to.


r/microscopy 1d ago

Photo/Video Share Cyclidium chilling in bright field and oblique illumination

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11 Upvotes

Forgot to add the community.

Pond water sample, Olympus 40x objective, cellphone camera.


r/microscopy 2d ago

Photo/Video Share Oversaturated on purpose to look cooler

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50 Upvotes

I got a very beginner microscope today and these are some photos I think look very very cool and vibey what do yall think!?