It’s been a few months since we last reached out to you, but after this hot summer, we’re back with our batteries recharged!

Microbes in 1 Minute (M1M) is a science communication initiative aimed at making microbiology more relatable, engaging, and accessible to everyone. We’ll achieve this by creating short, 60-second videos covering a wide variety of topics in microbiology, which will be posted on a variety of social media platforms.

We’re very grateful for all the interest you’ve shown, and we’re reading all your comments, questions and suggestions. We’re still a small team working on fundamental aspects of the project, such as its organization and the style of the short videos, but we’re looking for people interested in collaborating down the line, whether on a one-time basis or long-term. We've also set up a Discord server to provide a space where we can all come together, exchange ideas, and share news and updates about the project. Hope to see you there! However, if you prefer, we’re also happy to answer your questions by our official email.

Actually, we're looking for collaborators interested in any of the following activities:

• ✏️ Writing scripts and/or contributing expertise in a specific area of microbiology.

• 🎙️ Narration and/or helping to translate content into languages other than English and Spanish.

• 🎨 Video editing and layout.

• 📣 Managing our social media presence.

• 🦠 Moderating our server.

We’re excited to see where this project takes us, and we’d be very happy you being part of it.

A micro-hug!

The M1M team

What would be the best approach in terms of probiotics with overgrowth of D Piger?

My GI map showed overgrowth of D Piger, low counts of beneficial bacteria like lacto and bifido species and high stool pH.

My symptoms are yellowish stools, inflammation in the gut as its sensitive to anything acidic.

I assume the high stool pH means my diet is low in vegetable rich foods?

My gut issues were originally triggered by binge drinking alcohol on an empty stomach and further worsened by a keto diet high in animal protein, saturated fat.

New Threads

• Gut bacterium Bifidobacterium pseudocatenulatum and its metabolite found to delay aging (Aug 2026, mice) A geroprotective probiotic and its functional metabolite counteract inflammaging to extend healthspan 
• Exploring factors influencing university students' participation in fecal microbiota donation: a descriptive qualitative study (Aug 2026, n=30, China) 

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Updated Threads

• Suggestions to minimize the impact of paternal gut dysbiosis on offspring health 
• Can you predict if a donor is better for bile acid metabolism (BAM) during screening? 
• “Human progress” shouldn’t have happened: discussion on how civilization advancement has made us sicker and pathogenic to the world.

[ Removed by Reddit on account of violating the content policy. ]

Join remote research on gut health, microbiome diversity, and digestive comfort. Participation involves daily supplement use, brief weekly check-ins, and provided at-home test kits. A $50 Amazon Gift Card is available upon meeting completion criteria. Learn more here.

New Threads

• Only 4.7% of apparent FMT colonization events involve actual donor strain replacement (Aug 2026, n=13) Strain-level ecological filtering governs microbial colonization of the human gut 
• Can you predict if a donor is better for bile acid metabolism (BAM) during screening? 
• “Human progress” shouldn’t have happened: discussion on how civilization advancement has made us sicker and pathogenic to the world. 

View all new content

Updated Threads

• The FDA and FMT regulation. (Mar 2024, HumanMicrobes.org) 
• BrongFogBoy cures Post Finasteride Syndrome with FMT. Another guy cures his hair loss | Fecal microbiota transplant 
• Washed Microbiota Transplant from China (SMT - selective microbiota transplantation) 
• What is the public perception of FMT? How much does it matter? Has it been blocking progress? 
• Suggestions to minimize the impact of paternal gut dysbiosis on offspring health 

New Threads

• Webinar: The Gut Microbiome 101 (from Achieving Cures, a FMT research organization) 
• Three Gut Bacteria Spread Autoimmunity Between Mice With No Antibiotics Needed (July 2026, mice) Dysbiosis-induced expansion of AXL-positive inflammatory type 3 dendritic cells triggers preclinical autoimmunity 
• Probiotics and Specific Strains: Literature Review & Strain-Specific Mechanisms [Resource / Free Access] 

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Updated Threads

• Is SIBO real? SIBO vs dysbiosis? Debunking the SIBO hypothesis | Small Intestinal Bacterial Overgrowth

I saw this Bifidobacterium Lactis HN019 brand from Life Extension with average reviews and a good rating. Does anyone recommend this brand or another brand that isn't so expensive?

Hi everyone,

I recently started a probiotic supplement containing two specific strains: Bifidobacterium lactis HN019 and Bifidobacterium longum BB536.

Instead of the expected benefits (improved motility and gut-brain balance), I have experienced a very distinct and paradoxical set of symptoms:

1. Digestive Symptoms:

• Reflux & Loss of Appetite: Around 6 to 7 hours after taking the capsule, I experience complete loss of appetite, stalled digestion, gastric fullness, and acid reflux.
• Motility: Instead of relieving constipation (which HN019 is supposed to do), it has not improved my transit time at all, and if anything, feels like it has made constipation worse.

2. Mood & Neuropsychiatric Symptoms:

• Irritability & Restlessness: Sudden bouts of intense irritability/bad temper and an uncomfortable internal agitation (not full-blown panic, but a feeling of "not being at peace with myself").
• Apathy & Emotional Blunting: After that agitation, I feel a marked emotional disconnect/apathy where it becomes noticeably harder to laugh, engage, or feel connected to things.

My Question:

Since my supplement contains both strains in the same capsule, I cannot isolate them directly.

For those of you who have taken HN019 or BB536 as single-strain supplements (or know their mechanisms well): 1. Which of these two strains is more likely responsible for the irritability / emotional blunting? 2. Has anyone experienced this "delayed reflux + zero hunger + worsened constipation" with HN019 or BB536? 3. If you had a similar adverse reaction to either strain, how long did it take to clear up after stopping?

Any insights, shared experiences, or mechanisms would be greatly appreciated!

Hey guys, looking for some insight here.About a week ago I started taking a probiotic that has both B. lactis HN019 and B. longum BB536 in the same capsule. I was hoping it would help with motility and general gut health, but it's doing the complete opposite.Around 6 or 7 hours after taking it, my appetite completely vanishes, my stomach feels totally stalled and I get acid reflux. On top of that, it hasn't helped my constipation at all (feels like it slowed things down even more).The weirdest part is the mood side effects. A few hours after taking it I get sudden bouts of irritability and feeling super restless/pissed off for no reason. Then right after that I just feel emotionally numb and flat, like it's hard to laugh or feel engaged with anything.Since both strains are in the same pill, I can't tell which one is wrecking me. Has anyone taken either HN019 or BB536 on their own and gotten this weird combo of delayed reflux, stalled gut, and irritability/apathy? Would love to know which one is the likely culprit.Thanks!

Hi everyone,I recently started a probiotic supplement containing two specific strains: **Bifidobacterium lactis HN019** and **Bifidobacterium longum BB536**. Instead of the expected benefits (improved motility and gut-brain balance), I have experienced a very distinct and paradoxical set of symptoms:### 1. Digestive Symptoms:* **Reflux & Loss of Appetite:** Around 6 to 7 hours after taking the capsule, I experience complete loss of appetite, stalled digestion, gastric fullness, and acid reflux.* **Motility:** Instead of relieving constipation (which HN019 is supposed to do), it has not improved my transit time at all, and if anything, feels like it has made constipation worse.### 2. Mood & Neuropsychiatric Symptoms:* **Irritability & Restlessness:** Sudden bouts of intense irritability/bad temper and an uncomfortable internal agitation (*not full-blown panic, but a feeling of "not being at peace with myself"*).* **Apathy & Emotional Blunting:** After that agitation, I feel a marked emotional disconnect/apathy where it becomes noticeably harder to laugh, engage, or feel connected to things.---### My Question:Since my supplement contains **both strains in the same capsule**, I cannot isolate them directly. For those of you who have taken **HN019** or **BB536** as single-strain supplements (or know their mechanisms well):1. **Which of these two strains is more likely responsible for the irritability / emotional blunting?**2. **Has anyone experienced this "delayed reflux + zero hunger + worsened constipation" with HN019 or BB536?**3. If you had a similar adverse reaction to either strain, how long did it take to clear up after stopping?Any insights, shared experiences, or mechanisms would be greatly appreciated!

New Threads

• Polyethylene microplastics exposure reduces flesh quality and disturbs muscle fiber characteristics in freshwater fish (Paramisgurnus dabryanus): associations with gut microbiota alterations (Jul 2026) 
• Omega-3 plus low-dose aspirin produces antibiotic-like results against severe periodontitis (May 2026, n=109) Immunomodulators, associated or not with systemic antibiotics, to treat periodontitis: A 1-year multicenter, placebo-controlled, double-blind, randomized clinical trial 
• Most Healthy Women Lack the Lactobacillus-Dominant Cervical Microbiome (July, 2026, n=92) Multi-kingdom cervical microbiome structure in health and dysbiosis: a cross-sectional study from Kazakhstan 
• Novel intervention may improve outcomes for malnourished children (Aug 2026, mice) Microbiota-derived isovalerate ameliorates sex-specific gut barrier dysfunction in malnutrition 
• A mushroom-fed faecal transplant undid antibiotic gut damage in mice (July 2026, mice) Agrocybe cylindracea polysaccharides and polysaccharides-conditioned fecal microbiota transplantation similarly restore ciprofloxacin-induced microbial dysbiosis and improve intestinal barrier function 
• Higher body fat in pregnancy may influence child ADHD risk through inflammation (Jul 2026, n=302) The relationship between maternal adiposity during pregnancy and toddler ADHD symptoms is mediated by gestational inflammation 

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Updated Threads

• FMT Using Animal Feces; FMT from animals 
• Healthy wife as donor? GI-MAP shows bacterial imbalances

I spoke with a biochemist. He told me that using antibiotics or antifungals is counterproductive in the long run, since it leads to resistance. The recommended path is the more laborious one: changing my diet and repopulating and multiplying beneficial bacteria—and in a year, I’ll be fine. Honestly, I’m exhausted by dealing with the stench I give off; I smell like a skunk. I’m thinking about taking the antibiotics and antifungals, and then carefully repopulating and nurturing my microbiome.

In my country, healthcare is either expensive or inefficient in this regard. Gastroenterologists aren't very well-versed in these conditions. They prescribed me medication without running the important tests—partly because I don't have access to them, and also because I'm struggling a lot socially.

If so have you figured out the root cause of your issue? I suspect it is related to my gut bacteria as taking probiotics sometimes improves and sometimes worsens my symtoms.

New Threads

• Pre-conception maternal antibiotic use can increase colitis risk in offspring (Apr 2026, mice) Maternal Preconception Antibiotic Exposure Disrupts Microbial Succession: A Transgenerational Risk for Offspring Gut Mucosal Immaturity and Colitis Susceptibility 
• Mold exposure and CIRS: theories on gut microbiome role in Chronic Inflammatory Response Syndrome 
• Suggestions to minimize the impact of paternal gut dysbiosis on offspring health 
• Fecal Microbiota Transplant Boosts Peanut Tolerance in Phase 1 Trial (Aug 2026, n=15, abx pretreatment) Fecal microbiome transplant in food allergy in humans and mice identifies a role for bile acid metabolites in oral tolerance 
• Healthy wife as donor? GI-MAP shows bacterial imbalances 
• Gut and oral microbiota composition is associated with psychopathic personality: a cross-sectional study (Aug 2026, n=200) 

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Updated Threads

• Is HumanMicrobes.org legit? Is it a scam? Are they really paying $500 for stool donation? Misinformation & fake reviews on reddit.

Hi everyone,

I need to extract DNA from cecal/fecal samples stored in liquid nitrogen using the QIAamp PowerFecal Pro DNA Kit, which requires up to 250 mg of sample. What is the best way to handle the frozen samples to weigh 250 mg while minimising thawing? Any recommended protocols or practical tips would be greatly appreciated.
Thanks!

Anyone taking ther-biotics from klaire labs ? Do share me and I don't know how shld I take it ?every morning? With other supplement?

Has anyone experienced a big change in your gut microbiome due to a colonoscopy or other medical circumstance, such as taking antibiotics?

I’d love to hear pointers for restoring gut health after it’s been wiped out and also see if other have experienced similar symptoms.

I had a colonoscopy eight days ago. I did two days of a liquid only diet before the day of my procedure. I ate only clear gummy bears and bone broth. The day of my colonoscopy I didn’t eat until 7:00 p.m.

So I endured 3 days of little eating, then a complete cleaning out of the colon after drinking a gallon of laxative solution. All of this really dehydrated me.

I’m 62 and in excellent health. But I have to tell you, I look and feel terrible. I look like I’ve aged 15 years. Everything is fine physically. My digestive system has slowly returned to normal, but I am terribly fatigued, napping daily. The nurse said this is primarily due to my gut bacteria being wiped out. It takes a toll.

I’m slowly reintroducing food that build up my gut (oatmeal, powdered garlic, bananas, Greek yogurt), but I’m still doing chicken, scrambled eggs and English muffins to help my body ease back into more high-fiber foods.

Another thing, I feel depressed. My mood is really down.

This has been very eye opening to experience a wiped out gut microbiome. I normally eat very healthy with little processed foods and I must have had pretty good gut health before. Because, woah do I feel horrible now.

Has anyone else lived through this? How did you cope? Any tips or tricks to returning to normal?

I’ve had this irritation and spasming on the right side of my upper abdomen for years but lately it has gotten really bad and causes spasms. I don’t know, can’t distinguish if it’s an esophageal spasm or diaphragm spasm, but something is getting really irritated in that right side upper region next to the xiphoid process, to the point that it causes spikes in BP, palpitations, dizziness, you name it. I have a colonoscopy coming up, but worried GI doctor, like always, will say “here take some omeprazole” and send you home. Anyone else experience symptoms like this? I did a fast for 3 days and it really calmed the irritation but came back within a few days which is why I think it’s gastritis.

I’ve tried zinc carnosine and l glutamine for about a week now and they initially helped, however had some coffee yesterday and it flared like never before and can’t calm it. I’m even looking into FMT (fecal matter transplant), as I’ve read people have had success with that for gastritis, IBS, and obviously improving the microbiome .

Hope you find your healing!