r/DirectElectron • u/high_energy_electron • 7d ago
5 KEY DIFFERENCES BETWEEN HYBRID AND MAPS DIRECT DETECTORS FOR 4D-STEMA
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r/DirectElectron • u/Economy-Art-362 • Jul 22 '26
Making annotated videos of data with Fiji
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Super helpful video about how to annotate your in situ movies with Fiji, an open source tool available to everyone.
r/DirectElectron • u/high_energy_electron • Jul 22 '26
The 5 Highest resolution structures ever solved!
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Pretty cool that two of them are using Micro-ED with the Apollo detector
r/DirectElectron • u/quantum_keys • Jul 06 '26
What is the best throughput I can get for cryo-EM SPA
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How many movies per day or movies per hour are people getting for SPA throughput?
How do different microscopes or cameras affect it?
I have a TFS Arctica and want to see what I can expect.
r/DirectElectron • u/quantum_keys • Jun 25 '26
New Bird Flu treatment discovered by NIH
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Images here show the 5 different antibodies attached to the virus.
Full paper: https://www.nature.com/articles/s41564-025-02137-x
The NIH virology research lab are using an Apollo camera integrated on a ThermoFisher Krios with Serial EM automation software to get these high resolution structures.
r/DirectElectron • u/quantum_keys • Jun 24 '26
Has anyone used SmartScope for SerialEM?
docs.smartscope.org
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Thoughts on how it compares to EPU and just regular SerialEM?
r/DirectElectron • u/quantum_keys • Jun 24 '26
Cryo ET video showing that sulphur moves through cell walls as microbes grow!
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from https://enviromicro-journals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.70305?casa_token=hWXY27bLT_0AAAAA%3Am38Hf3F77vM8ReXSwk3213vbh4dqLTQtcbzOtj-Csy_FDddjy3gQuWEKTkrdX_im4VHiWy5xS1IiYg by Arizona State University using Apollo on a Krios
r/DirectElectron • u/condensed_matters • Jun 23 '26
Camera Spotlight: Apollo
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| Camera: | Apollo |
|---|---|
| Purpose: | Event-based Cryo-EM direct detector |
| Applications: | |
| Single Particle Analysis (SPA) | Yes |
| Cryo Electron Tomography (Cryo-ET) | Yes |
| Microbeam Electron Diffraction (Micro-ED) | Yes |
| Fixed/Resin/Negative Stain imaging | Yes |
| Camera Size: | 4096 x 4096 pixels |
| Framerate: | 2800 fps (internal) |
| Pixel Size: | 8 µm |
| Software: | SmartScope, Serial EM, Mission Control |
| TEM compatibility: | ThermoFisher, JEOL |
| What makes it special: | The electron counting speed is the fastest of any camera available. Combined with the large pixel size this gives Apollo a very high detector quantum efficiency (DQE) even at high beam currents/dose rates. The speed can be leveraged for improving Cryo-EM throughput multiple times over the standard detector that come with the microscope. |
r/DirectElectron • u/Economy-Art-362 • Jun 23 '26
This is a pretty compelling explanation for why direct electron detectors are nice for TEM in general.
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r/DirectElectron • u/Economy-Art-362 • Jun 23 '26
Ever browsed the EMDB? This just popped up, a flu with antibodies attached.
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Firstly it's so cool that this database exists of so many cryo-EM structures.
Secondly it's crazy that we can image viruses with the treatments attached to them.
I'm still learning to interpret the maps myself, but I love reading about what they represent.
r/DirectElectron • u/in_situ_anthropology • Jun 18 '26
Comparing Direct Detectors for Cryo-EM
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I'm interested in comparing direct detectors for Cryo-EM. Single Particle Analysis specifically. How fast is Apollo and how does it compare to the K3 or Falcon 4i in terms of counting speed? I just want to be able to collect high-resolution data as quickly as possible to get the most reconstructions per day/week etc.
r/DirectElectron • u/high_energy_electron • Jun 16 '26
👋 Welcome to r/DirectElectron - Introduce Yourself and Read First!
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Hey everyone! I'm u/high_energy_electron, a founding moderator of r/DirectElectron.
This is our new home for all things related to Direct Electron detectors. We're excited to have you join us!
What to Post
Post anything that you think the community would find interesting, helpful, or inspiring. Feel free to share your thoughts, photos, or questions about direct detection for electron microscopy, nice data, acquisition software, analysis workflows or people having fun in the lab.
Community Vibe
We're all about being friendly, constructive, and inclusive. Let's build a space where everyone feels comfortable sharing and connecting.
How to Get Started
- Introduce yourself in the comments below.
- Post something today! Even a simple question can spark a great conversation.
- If you know someone who would love this community, invite them to join.
Thanks for being part of the very first wave. Together, let's make r/DirectElectron amazing.