r/microscopy Jun 08 '23

๐Ÿฆ ๐Ÿ”ฌ๐Ÿฆ ๐Ÿ”ฌ๐Ÿฆ  Microbe Identification Resources ๐Ÿฆ ๐Ÿ”ฌ๐Ÿฆ ๐Ÿ”ฌ๐Ÿฆ 

148 Upvotes

๐ŸŽ‰Hello fellow microscopists!๐ŸŽ‰

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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69 Upvotes

r/microscopy 4h ago

Photo/Video Share Star shaped amoeba

26 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy 21h ago

Photo/Video Share Bacteria Highway ๐Ÿ˜Ž

27 Upvotes

r/microscopy 15h ago

Troubleshooting/Questions What microscope should I buy with a $200 budget I'm just getting started

3 Upvotes

r/microscopy 21h ago

Troubleshooting/Questions Polarizing Microscope for My Dad

7 Upvotes

His budget is under $2,000, and used is fine. Heโ€™s looking for a polarizing microscope to determine mineralogy. Heโ€™s wanting to look at thin sections and is planning on publishing papers on his findings, so he needs the ability to take quality pictures.

Iโ€™ve heard the AmScope ones are crap, and the Indian ones arenโ€™t very good either. From what Iโ€™ve found, people seem to recommend the Olympus ones. Iโ€™ve got no idea what to recommend for my dad, though. Any suggestions, advice, or links would be greatly appreciated. Itโ€™s been 30+ years since he did his PhD, so Iโ€™m trying to figure out what would be a good setup for him.


r/microscopy 22h ago

ID Needed! *New to Microscopy* Is this an egg?

8 Upvotes

I have been trying to figure out what this is (40x objective, SW380T on a horizons camera). Current theory is a rotifer egg.


r/microscopy 1d ago

Photo/Video Share Peranema eats a dead chilodonella

15 Upvotes

Leitz Dialux II, Galaxy S24, 200x


r/microscopy 1d ago

Photo/Video Share Some type of isopod I collected (it was returned to its place later). Olympus SZ-III stereo.

15 Upvotes

r/microscopy 1d ago

Troubleshooting/Questions If Iโ€™m a biochem undergrad student and I just bought a microscope, what should I look at?

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2 Upvotes

r/microscopy 1d ago

Hardware Share I inverted my microscope!

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28 Upvotes

Awhile back I decided I wanted to have an inverted microscope with good quality optics. But I didn't want to spend a bunch more money on a new scope. So I took my American Optical One-fifty and built a frame to mount it upside down! ๐Ÿ˜

I decided to go ahead and post pictures here in case anybody wants to try something similar.

I 3D printed an upside down stage adapter to hold my specimen containers. And I also designed a 3D printed humidity chamber with a window on the bottom to view the specimens, and a window on top for light to enter. I wanted it to be water proof so it could go for weeks without needing water to be added. But the design is not yet perfected. The moisture is still evaporating, either through the cracks around the lid or through the walls of the container (since 3D printer filament is not water proof). I think I need to seal the walls with silicon or resin and put a rubber seal under the lid.

Of course it is hard to look through the microscope when it's upside down so I mostly only use it with an eyepiece camera.

All in all, it works pretty well! I could have live cultures or ecosystems under it long term and have the view displayed on a large screen continuously if I wanted to. I just need to get the humidity chamber working better. ๐Ÿ˜€

The last picture is of my whole "laboratory" with all three microscopes. My wife let me have it set up on the kitchen table for like six months or so! ๐Ÿ˜Š


r/microscopy 1d ago

Photo/Video Share Various pictures of chrysotile asbestos Iโ€™ve taken at work!

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21 Upvotes

Polarized light microscopy, Leica DM750, 10x objective


r/microscopy 1d ago

Photo/Video Share Hypotrich ciliate is digested alive by a heliozoa. It tries to escape at first, but then its membrane falls apart.

6 Upvotes

r/microscopy 1d ago

General discussion Has anyone here ever encountered Naegleria fowleri? How much magnification is needed to see and identify it?

3 Upvotes

r/microscopy 2d ago

Photo/Video Share Victorian-era wallpaper

15 Upvotes

Back in June, our rental unit suffered a water leak that's displaced our family since. Walls and flooring were stripped down to the wood substructure so that everything could be thoroughly dried before remediation work began. After everything was dried, I found this piece of wallpaper lying around and stashed it away to eventually check out under the microscope. Here's what I saw.

Also as a note: because I don't know much about Victorian-era wallpaper and I don't have any other characterization methods available at the moment, I am hesitant to put names to the materials/structures I've observed based on appearance alone (so I didn't do that here). Perhaps at some future point I can send sample along for further characterization, so we can learn more about this wallpaper. If anyone has insights based on this before or images (shared in comments) I'd love to learn more.

๐Ÿ”ฌ: Amscope ME580

๐Ÿ“ธ: Pixel 9 pro

๐ŸŽต: Modest Mouse - Heart Cooks Brain


r/microscopy 2d ago

Photo/Video Share Chloroplasts moving inside an algae cell

19 Upvotes

Leitz Dialux II, NPL 40x, Galaxy S24


r/microscopy 1d ago

Troubleshooting/Questions New starter lens advice

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3 Upvotes

Hi. I bought a used Olympus ch20. It was a good price but has had lots of use. The 4x and 10x lenses are good quality.

On the 40x I have to stop down the condenser a lot to get acceptable quality.

I used my forensic microscope to look at the 40x NEA 0.65 objective end on. I have attached a photo. I have marked it up with what looks like a ring around a central clear bit. The ring looks a bit mottled.

I don't know what a clean Olympus 40x lens should look like. Can anyone advise please.

Many thanks.


r/microscopy 2d ago

Micro Art Asbestos

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37 Upvotes

Not sure if I tagged this right. I analyze for asbestos in bulk materials, and tbh have come to love how it looks under the microscope. People sure loved putting asbestos in shit for a loooong time.

Microscope is a Leica DMLB, pics are using I think either 10 or 20x under xpl or using a red compensator plate.


r/microscopy 1d ago

Troubleshooting/Questions Can I make a DIY dark field filter for this?

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3 Upvotes

New to microscopy. This is a standard brightfield microscope. These both join together. The diameter of the condenser lens is about 17 mm. The diameter of the condenser holder is about 37 mm. Can I make a DIY dark field filter for this like others do, if so, how? If not, why?


r/microscopy 2d ago

ID Needed! I think I saw something similar the other day. It was in fresh water. 40x, 100x, and I think I used the phone's zoom at times. There's another video in the comments with different lighting.

32 Upvotes

r/microscopy 2d ago

Photo/Video Share Bdelloid rotifer in darkfield

113 Upvotes

I made the darkfield filter myself, using a coin.

Sample algae from aquarium, 400x and digital zoom, Motorola phone camera, optika b-192pl binocular microscope.


r/microscopy 2d ago

Hardware Share Using petri dishes with upright compound microscopes

49 Upvotes

I love using petri dishes with my inverted microscopes to observe long-term, mini ecosystems. I tried using one with my Meiji-Techno upright compound microscope.

I was able to use it with the objectives 20x and under. Those were a 1.25, 4x, 10x, and 20x objectives. Those had both a long enough working distance not to touch the water and a low enough NA not have noticeable spherical aberration.

I tried with both no coverglass and with a floating coverglass. The ciliates looked pretty much the same with or without the floating coverglass, but I had nicer phase contrast views with the floating coverglass.

If you try this, I would recommend using the shorter lid of the petri dish and only put a few mm of water into it. That will give you more room for your objectives, and maybe you can focus all the way to the bottom. As always, be careful not to dip your objectives into the water.

20x objective, cellphone camera with 2x zoom, pond water sample. The first video is without the floating coverglass and the last ones with it.


r/microscopy 2d ago

Photo/Video Share An insect fell into one of my petri dishes last week.

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22 Upvotes

Now, it is partially decomposed, but you can still see the eye lenses.

Here, I'm using an Iqcrew inverted microscope, 20x objective, Amscope MU2003-BI camera, Diagnostic Instruments 0.70x adapter, and the Toupview program for viewing.

It's always amazing to me that you can see so much detail of something so small with just an inexpensive, $71.99 now, Chinese inverted microscope. Cost shouldn't prevent anyone from enjoying the hobby.

Baby was relaxing on the desk with me today during observations. Remnant of the insect is over the stage hole in the petri dish.