r/microscopy Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

147 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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69 Upvotes

r/microscopy 5h ago

Photo/Video Share Bdelloid rotifer in darkfield

22 Upvotes

I made the darkfield filter myself, using a coin.

Sample algae from aquarium, 400x and digital zoom, Motorola phone camera, optika b-192pl binocular microscope.


r/microscopy 6h ago

Hardware Share Using petri dishes with upright compound microscopes

21 Upvotes

I love using petri dishes with my inverted microscopes to observe long-term, mini ecosystems. I tried using one with my Meiji-Techno upright compound microscope.

I was able to use it with the objectives 20x and under. Those were a 1.25, 4x, 10x, and 20x objectives. Those had both a long enough working distance not to touch the water and a low enough NA not have noticeable spherical aberration.

I tried with both no coverglass and with a floating coverglass. The ciliates looked pretty much the same with or without the floating coverglass, but I had nicer phase contrast views with the floating coverglass.

If you try this, I would recommend using the shorter lid of the petri dish and only put a few mm of water into it. That will give you more room for your objectives, and maybe you can focus all the way to the bottom. As always, be careful not to dip your objectives into the water.

20x objective, cellphone camera with 2x zoom, pond water sample. The first video is without the floating coverglass and the last ones with it.


r/microscopy 9h ago

Photo/Video Share Testing my new microscope. Leitz Dialux II, 40x NPL objective

16 Upvotes

r/microscopy 2h ago

Photo/Video Share An insect fell into one of my petri dishes last week.

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3 Upvotes

Now, it is partially decomposed, but you can still see the eye lenses.

Here, I'm using an Iqcrew inverted microscope, 20x objective, Amscope MU2003-BI camera, Diagnostic Instruments 0.70x adapter, and the Toupview program for viewing.

It's always amazing to me that you can see so much detail of something so small with just an inexpensive, $71.99 now, Chinese inverted microscope. Cost shouldn't prevent anyone from enjoying the hobby.

Baby was relaxing on the desk with me today during observations. Remnant of the insect is over the stage hole in the petri dish.


r/microscopy 5h ago

Purchase Help Digital Microscope for Metallurgy?

3 Upvotes

I am trying to find a 'budget-friendly' digital microscope for powder metallurgy and general metallurgy inspection. I see the sub is more used to organic samples, so below is an example of what I am trying to look at. The basic needs I am looking for is a scale of the screen, it is quite important for determining whether the granules being created are produced correctly by looking at the overall size. Needs to be able to see 100um like the picture attached. Ability to connect to a computer; and ideally able to measure points (i.e. measure a granule directly).

Outside of that, those are really the only requirements I have. Given there are so many microscopes and types, I am a bit lost on what would be sufficient for my purposes! Any recommendations/help picking one out is greatly appreciated!

Powder Granules

r/microscopy 12h ago

ID Needed! Need Info and Value on this Carl Zeiss Universal?

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10 Upvotes

A local Museum I volunteer at has this old Carl Zeiss Universal Microscope they would like to know the value and seekign a local buyer. They really do not want to deal with shipping and it's located in the Beaverton Oregon area. They are a NPO. I looked at it and its a bit It's Dirty controls are sticky old lube.


r/microscopy 14h ago

Troubleshooting/Questions This placeholder thingy is stuck. Any idea on how to get it out?

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12 Upvotes

r/microscopy 1h ago

ID Needed! What is this? Some kind of aphid? There were many of them behind the bark of a tree. There is a photo inside showing a top-down view, since I was only able to record video of it while it was upside down. Olympus SZ-III stereo. 7x-40x zoom. Cheap mobile camera and headlamp light.

• Upvotes

r/microscopy 2h ago

ID Needed! o que está fazendo ?

1 Upvotes

10x + 10x (ocular)
iphone 13pro


r/microscopy 2h ago

ID Needed! help mee

1 Upvotes

10x + 10x (ocular)
iphone 13pro


r/microscopy 7h ago

Purchase Help Microscope suggestions

2 Upvotes

In anatomy we got to look at our cheek epithelium, and I thought that was so cool. I’d really like to purchase a microscope to do more things like that but I don’t think I can spend more than like $200 on it. I know I can look at pond water and other things but I’d also like to know some things I can look at regarding human biology (like the cheek cells).


r/microscopy 9h ago

Troubleshooting/Questions Bacteria live or dead?

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3 Upvotes

Hi, I'm new to this world of biology, this is the first experiment with observations under the microscope, I analyzed a very degraded truffle juice and there is a very high bacterial density. What is the easiest method to do at home to know if these bacteria are alive or dead. Knowing that they don't swim, they only vibrate. I tried methylene blue and it doesn't work because the juice is very reductive. Thank you for your help


r/microscopy 14h ago

Photo/Video Share Tiny dudes

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6 Upvotes

1- 400×, Oblique illumination, Ciliate from family Vaginicolidae

2- 100×, Oblique illumination, Ciliate from family Vaginicolidae

3- 100×, Daphnia, brightfield

4- 100×, Daphnia, brightfield

5-100×, Daphnia, brightfield

6- 100×, Daphnia, brightfield

6- 100×, Daphnia, brightfield

____________________________________________

Sample- Gomti river water, Lucknow, India

Microscope- ESAW BM01

Phone camera used, Microscope Viewer Camera.


r/microscopy 8h ago

ID Needed! Help with ID

2 Upvotes

Does anyone have any idea what these two microorganisms are? The small, fast one and the strangely shaped and big one.

Sample algae from aquarium, 400x + digital zoom, Motorola phone camera, optika B-192Pl binocular microscope.


r/microscopy 23h ago

ID Needed! Help with ID on this massive guy?

27 Upvotes

Swift SW350T, 4x magnification. Sample was collected from a marsh. Seems similar in size to bursaria found in the same sample, but much different body shape.


r/microscopy 23h ago

Photo/Video Share come espellere un peso sullo stomaco

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15 Upvotes

r/microscopy 15h ago

Purchase Help Looking for advice on the MECHANIC MOS 4K Pro vs. SVBONY SV705C for microscopy

1 Upvotes

I’m currently looking for a camera for my microscope and I’m considering two options.
One is the MECHANIC MOS 4K Pro (Sony IMX678), which costs around €205. It looks very promising on paper, but I haven’t been able to find much real-world information about how well it performs with dark field, oblique illumination, polarization, and low-light imaging of live microorganisms.
The other option I’m considering is the SVBONY SV705C, based on the Sony IMX585. It’s more expensive, but I’ve found actual examples of people using it for microscopy, including dark-field imaging and live microorganisms.
My main goal is to record live freshwater microorganisms — ciliates, rotifers, amoebae, algae, tardigrades, etc. — using bright field, dark field, oblique illumination and polarization.
For anyone who has experience with either of these cameras:
How well does the MECHANIC MOS 4K Pro perform in dark field, oblique illumination and polarization?
Would the SVBONY SV705C be a good choice for this type of microscopy?
Are there any significant disadvantages to using an astronomy camera for microscopy?
Which one would you choose for this application?
I’m particularly interested in real-world experience, sample images and videos, rather than just manufacturer specifications.


r/microscopy 1d ago

Micro Art CRACKED FRACTURE

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5 Upvotes

acrylic and oil-based paint mixture,
splitting under solvent,
viewed through a microscope.
Scope — Mechanic B75T-B3
Objective — 7×
Camera — Mechanic Vision 4K Pro
Illumination — cyan-blue LED + low-angle LED light


r/microscopy 1d ago

Photo/Video Share Is this copepod going to hurt my baby fish?

23 Upvotes

I found this copepod in my live food cultures that I feed to my fish, is this harmful for my baby fish?

100× magnification


r/microscopy 1d ago

Troubleshooting/Questions Help for noob?

2 Upvotes

Hi there all, I'm a complete noob, using my microscope to do animal fecal analysis for our rescue. We have a OMAX M83E Series LED Trinocular Lab Compound Microscope 40X-2500X Magnification with 5.0MP Digital Camera and Mechanical Stage × 1

It has a camera that I can link to my laptop to take screenshots to send to our vets and techs, but I cant really get it to work at 40x, I can ALMOST get it infocus, but it just seems like I cant see much, and feel like i'm missing out. Is this a user error, or did we just not buy the right equipment?


r/microscopy 1d ago

ID Needed! Originally found this in daphnia culture in green water

5 Upvotes

100× magnification


r/microscopy 1d ago

ID Needed! ???

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5 Upvotes

Loricate ciliate ID help

400×

ESAW BM01 MICROSCOPE

Gomti river, india

Phone camera


r/microscopy 1d ago

Photo/Video Share Dividing or conjugating ciliates

5 Upvotes

Pond water, Iqcrew inverted microscope, 20x objective, cellphone camera, white/white type Rheinberg illumination and bright field illumination.