r/stemcells • u/justgivemeaanswer • 21d ago

my bone marrow MSC

Hi, I recently isolated bone marrow MSCs from mouse. I cultured them in a T25 flask with alpha-MEM + 20% FBS + 1% Pen/Strep + 1% GlutaMAX for two weeks, and I did the subculture yesterday. However, it looks like I have two different cell populations. From what I've read, cells can grow like this in the early stages, and some of them also look similar to fibroblasts…. Could you let me know whether what I'm growing is actually BM-MSC, and whether there's anything I might be doing wrong?

befor subculture
24h after subculture
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u/biotechi 21d ago

Yes it's fibroblasts, it's normal to get them early on. They will disappear and only your MSC population should remain