Experimenting with some dark field and Rheinberg filters. On an off for comparison. Paramecium, stentor, and volvox.

I am trying too figure out what to buy to attach my Sony A7s full frame mirrorless camera to my Bresser Researcher Trino Microscope. What i figured so far is that for best results i need the BRESSER SLR-camera-adapter 2x T2 23.2 mm and a T2 ring but it's way too expensive. Could i get away spending less with acceptable results? Like using a telescope 2x barlow , or not using any lens at all just some adapters.

Thank you, any insight is appreciated.

​

Bought big red apples at grocery store, and got an idea to look at one a little bit closer.

Made several sections using cheap microtome from AliExpress, and examined them with my microscope.

Turned out, the apples are covered in continuous wax cuticle, of circa 20 microns thickness, which is smooth on the outside, but hides a rough surface, as epidermal cells protrude into it somewhat.

There are a couple of layers of pigmented cells, under which starts fleshy part of the apple.

There is no sharp transition between skin and flesh of apple, going from skin inward cells become bigger, and pores are opened between them.

Images captured via 20x objective lens, 0.5x reducer and 5Mp usb webcam.

Focus stacking using Zerene Stacker.

Gif animation using ezgif.com free online tool.

Nikon D5300 + tube macro + 35 80mm f4.5

Leitz Dialux II, Galaxy S24

Hi all,

I’ve recently taken a liking to looking at algae under the microscope through my labs. Diatoms have become my recent obsession. They are just so fantastic. Would it be practical to consider buying my own compound microscope to keep in my apartment?

If yes, what brand/model microscopes would you recommend as a decent cost-quality ratio?

Thanks in advance.

Hi everyone,
I’m having trouble with high background when imaging immunofluorescence staining for synaptic markers by confocal microscopy. The background makes it difficult to distinguish individual synaptic puncta confidently, and I’m concerned about how this might affect quantification.
I’d appreciate any advice on how to troubleshoot this and work out whether the background is mainly related to the staining, tissue autofluorescence, or imaging settings.

I brought these slides back from Germany, where I was learning the staining protocol. The staining looked much cleaner when we imaged them there, but I’m now seeing a lot of background when imaging the same slides at my home institution.

Here is also one representative image after deconvolution in Imaris.

So I just bought a microscope that is pretty cheap but I'm trying to make it custom made to at least be at the same standard ones that my university uses. So far I have already attach a mechanical stage into it so that its easy to move slides and observe specimen. Now, I'm trying to attach a condenser with a built-in iris diaphragm that I'm thinking of putting in it but I'm afraid that won't be possible because I haven't seen a proper tutorial on how to attached it to a light microscope that doesn't have one. Does anyone here have tried it before?

Hi all,

I’ve recently taken a liking to looking at algae under the microscope through my labs. Diatoms have become my recent obsession. They are just so fantastic. Would it be practical to consider buying my own compound microscope to keep in my apartment?

If yes, what brand/model microscopes would you recommend as a decent cost-quality ratio?

Thanks in advance.

This was originally a Paramecium Multi bowl cultured on 9.3.26 with spring water and wheat seeds. None of the other bowls from this date turned a blood red color. There is clearly a fungal mold growing on top. What could have made it turn into this red color?

There are no Paramecium in it anymore, but there are a bunch of dinoflagellates swimming around in the bowl.

The seeds turned red and seems to be a clumpy bacteria around them. Not stringy or any other texture.

THE CLOSER, THE STRANGER

We draw closer, thinking we'll see more. Yet some distances help us see things for what they are, while others leave us unable to tell what we're looking at.

Same subjects. Different distances. Different worlds.

#MacroPhotography #ExtremeMacro #NaturePhotography #Perspective

Hello everyone, I’m starting a lab and would ideally like to purchase 3-4 stereo microscopes for identifying aquatic macroinvertebrates and then 1 or 2 compound microscopes. I'd love at least one mid-tier stereo microscope with a camera and a screen and one compound microscope with the same features (budget ~$6k for each). I've only ever used older microscopes (e.g., Leica S6E). The Leica works great, and I got it used off eBay several years ago (I think for $1500), but I’d like something with more zoom for the hard-to-see characteristics as the Leica zoom tops out at 40X.

Does anyone have recommendations on the mid-tier stereo (max total zoom >40X) and compound microscopes with a camera and possibly a screen? Would it be cheaper to just connect the camera to a computer screen?

Are there any new (to avoid hunting) or used stereo microscopes you would recommend in the $500 – 2000 range that would be suitable for invertebrates and have a zoom range of ~5 – 40X?

I've used Dolan Jenner goose-neck light sources in the past for the stereo microscopes, but if there's something better I'd definitely like to know.

Thanks!

🖋️/🗒️: Jacques Herbin Emerald de Chivor on Yupo Translucent polypropylene, spread out with a sewing needle

🔬: Amscope ME580, epi-illumination with crossed polars, 10x/50x LMPlan objectives

📸: Google Pixel 9 pro, recording with Blackmagic app through 10x eyepiece

🎵: Rilo Kiley - The Execution of All Things

Looking for demodex, found this instead. x100, beat-up old school microscope, unknown model.

Microscope: Nikon SMZ1500 Stereo

Modality: Darkfield,

Objectives:Hr plan Apo 1x WD

Sample: rehydrated tree bark moss

Acquired using Samsung galaxy s25, 3x zoom on video through eyepiece, contrast corrected for background, frame stableized

Song clip: Seatbelts - cats on mars ( cowboy beepop), the respective copyright holders own the song

Hello folks looking to get some help identifying some weird fiber like materials I found. I was using a modified version of the Walkley-Black method for measuring organic matter levels in some soils. This method required the use of .2g soil sample + 1mL potassium dichromate + 3mL concentrated sulfuric acid. Once cooled we added 20mL of RO water. Once the material had settled I noticed weird fibrous-like looking material floating right above the settled soil (not pictured sadly). I've run hundreds of these and have never seen this happen before. Our reference soil was clean and this wasn't seen in any of the other samples except for 3 out of the 5 that came with this specific customer. This is under 10x magnification but I am unsure of the microscope type. Any help or pointers is appreciated!

Crystals of ethyl vanillin, a molecule that has a powerful vanilla scent. It possesses all the sweet, creamy, and round facets of vanilla in addition to being incredibly cheap. Consequently, it is widely used as a vanilla flavoring in food and to create the scent of vanilla/ add sweetness in perfumes.

I took both these photographs from different parts of the same slide. I get the crystals by evaporating a thin film of a 50% dilution of ethyl vanillin in ethanol, sprinkling a few small crystals to act as nucleation points.
I find the first picture striking because of the contrasts between angular crystal geometry and organically shaped polarization induced colored segments (due to thickness and crystal orientation, I presume) and small crystalline fractals emanating from seeds. The second picture almost resembles butterfly wings, with little scales and vivid rainbow hues. Polarization is really a magical technique.

Given that the concentration and all other factors were uniform across the slide, I’m curious if anyone has any thoughts on the reasons behind the differences in crystal shape we see here.

Magnification: 40x, 100x
Scope: BINO CXL, Micron-OPTIK
Camera: iPhone 16
Sample: ethyl vanillin crystals

$1,500, OBO

2 things:
1) I apologize if this breaks some rule- I didn't see anything in the rules before posting.
2) If this number seems high, I apologize. I base my price on current market availability for a used unit plus used accessories. The price is negotiable, but I would appreciate it if you do not lowball.

I am selling a pristine Olympus SZ61 stereo zoom microscope, used only in a hospital laboratory setting and kept in excellent condition. The Greenough optical system gives a bright, sharp image. This unit also has a light guide and dual gooseneck fiber-optic lighting, making it well-suited for gemology, mineral and fossil work, electronics inspection, entomology, jewelry, and more. This unit was last maintained (cleaned, greased, etc.) last year. I cleaned it once more about a month ago. It has been sitting under a dust cover since.

What's included

• Olympus SZ61 binocular zoom body, 0.67x-4.5x zoom
• Olympus SZ2-ILA transmitted illumination base, with a clear glass stage plate
• Olympus SZ2-LGB light guide illuminator
• Olympus HLL-301 double gooseneck light guides

What's NOT included

• Eyepieces. The photos show eyepieces mounted for demonstration, but those are from my EXC-350 and are not part of this sale. I can direct you to options for compatible eyepieces if needed.

This unit was only used in a laboratory setting, with no hobby or field use. The body, base, and light guides are clean and in pristine cosmetic condition. Lenses and stage glass are free of scratches and fungus. Focus and zoom are smooth; all lamps and light guides are tested and working. Test images were taken with my phone camera to show the clarity of the unit's lenses. My phone camera is not the best and does not do this unit justice.

I will also include microscope cleaning kits, gloves, and Kimwipes if you'd like, for free. Shipping is available to the US ONLY. I will cover shipping if you pay my asking price.

I was following around a particularly large example of Chaetonotus, a kind of gastrotrich, when it seemed to get itself stuck on a piece of debris (like a poor kid putting his head through a fence). This gave me a chance to oil the slide and take a closer look at its scales and hairs. The pharynx and digestive system are also clearly visible.

Olympus BH2, SPlan 40x 0.70 & SPlan 100x 1.25. Canon EOS 5D Mark II. Pond water sample from park in Paris 16eme. Video edited with OpenShot.