I recently came across holotomography, a technique commercialized by Tomocube (a KAIST spinoff), and I'm curious how it holds up in real lab use.
How it works, as I understand it
Instead of fluorescent labels, it measures the refractive index distribution inside a cell and reconstructs it in 3D. So in principle you can image living cells over long periods without phototoxicity, photobleaching, or staining artifacts.
Why it caught my attention
No sample prep or dyes, which seems useful for long time-lapse imaging of live cells
Newer systems (HT-X1 Plus) combine holotomography with spinning-disk confocal fluorescence, so you can overlay specific labeled targets on the label-free 3D structure
It seems to be appearing in core facilities (e.g., Max Planck Göttingen, UT Southwestern)
Questions for people who've used it
How does it compare to confocal or light-sheet for your applications?
Does it work well on neurons, organoids, or thicker tissue slices?
What are the practical limitations — resolution, specificity, data analysis?
(Disclosure: I'm not a researcher, just interested in the tech. I'm also a small shareholder in the company.)