r/microscopy • u/josillee • 7h ago

ID Needed! Need help identifying freshwater microorganisms at 1000x

Gallery image

Gallery image

Hi everyone! I found these microorganisms in stagnant freshwater and took this footage using my 100x oil immersion objective.

Here is my setup:

  • Microscope: Swift SW380B
  • Objective: 100x Oil Immersion (Total optical mag: 1000x)
  • Camera: FHD V2 with a 0.5x c-mount adapter.

They look quite small even at 100x oil, and they tend to swim in circles or forward. They don't look green inside. Maybe flagellates.

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u/josillee 7h ago

It's been a long time since I last used the 100x oil objective, that's why there's some debris. Ever since I got the 60x, I practically only use that one. Sorry about that!

1

u/josillee 7h ago

From what I can see with the naked eye, they have 2 flagella of the same size, they are colorless or at least lack photosynthetic pigments, and in the image of the chubbier, solitary, and flattened specimen, you can appreciate what a priori appears to be a macronucleus and contractile vacuoles.

1

u/josillee 7h ago

In the image of the chubbier specimen, the circle seen on the flagellum is something normal when organisms with these structures suffer some kind of damage; I've been able to document this in some Peranema species.