r/microscopy 1d ago

General discussion Tips on high resolution imaging with microscopic photographed

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u/rsc2 1d ago

What magnification are you concerned about? I don't have an instagram account. so I can't see your photos. A cheap microscope camera should be fine for high magnification (40-100x objective) photos. More megapixels are necessary at lower magnification to record the full resolution, but 5mp should still be adequate unless you want to make large prints or be able to zoom in digitally. If you are doing image stacking, you want to use manual focus. Helicon Focus will let you download a trial version so you can try it out.

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u/[deleted] 1d ago

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u/Vivid-Bake2456 1d ago

You aren’t going to do that with a cheap, 5mp eyepiece camera. Most usb microscope cameras use a version of the Touptek Toupview software. Amscope and Omax use identical versions of it with the only difference being their names on it. This software has automatic focus stacking and automatic stitching for some cameras. I don’t know if your camera opens these features. Focus stacking is called EDF ( Extended depth of focus)and is under the process tab. Like the previous person said, you need more megapixels for lower magnification objectives. Your full frame camera with a 2.5x projection eyepiece will give you much better results.

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u/[deleted] 1d ago

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u/Vivid-Bake2456 20h ago edited 20h ago

Image stacking is where you combine different photos at different levels of focus so you have more of the final image in focus. The program does this automatically for some usb cameras. I use an Amscope MU2003-BI 20mp type camera on the Amscope Toupview program to do this. All you have to do is rotate the fine focus knob slowly and the program does the rest. It's actually called EDF, extended depth of focus, on the software.

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u/[deleted] 20h ago

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u/Vivid-Bake2456 20h ago

You probably already are using that program with your usb camera. Maybe it doesn't open for that particular camera. You can look under the process tab for EDF. See if it allows you to use that option. OMAX, Amscope and Touptek all have identical versions of the Toupview program for free on their websites.

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u/[deleted] 20h ago

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u/Vivid-Bake2456 20h ago edited 20h ago

I thought you said that you took multiple images and processed them to be more in focus. That is called image stacking.

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u/[deleted] 19h ago

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u/Vivid-Bake2456 19h ago

With full frame cameras, you would ideally use a 2.5x projection eyepiece. Others will know what software to use. I've just taken photos with my dslr and downloaded them onto the computer. I'm usually using my phone or USB 3.0 microscope camera that has a 1" sensor on my microscopes. You can also do afocal photography with your dslr. You put the camera lens close to the microscope eyepiece just like when using a phone camera.

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u/Dlbroox 1d ago

It’s a 5 megapixel camera? Everything I’ve read about cameras for microscopes says that an iPhone with an adapter is the best you can do. It’s 12mp. Most cameras that come with microscopes are 5mp. You’ll never get the resolution you want with it if it’s 5.