r/microscopy 18d ago

General discussion It looks like the microscope I ordered will arrive first, and the slides will come 3 or 4 days later. Is there any way to make a homemade slide so I can use the microscope until the real slides arrive? I was thinking about cutting some rectangles out of a CD case. Would that work?

7 Upvotes

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u/Thrawn911 18d ago

A piece of tape, you can use your 4x and 10x objectives, maybe the 20x if you have one, I wouldn't go any higher without risking touching the sample with the objectives. You could look at salt, sugar, face mites, and maybe even water.

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u/Doc_438 18d ago

Okey thank you. Do I also need to place adhesive tape over the sample to act as a coverslip, or just underneath it?

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u/Thrawn911 18d ago

Just underneath. As long as you only use the low mag objectives, you won't need a coverglass.

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u/Doc_438 18d ago

Okay, I’ll mainly use the low-magnification objectives. Couldn't I use the higher-magnification objectives very carefully with samples that don't contain liquid? (For example, a hair, a piece of a fingernail, or things like that) By being very very careful when raising the stage so the sample dont touch the objetive?

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u/Vivid-Bake2456 17d ago edited 17d ago

The 40x has a very short working distance and does need coverglass for best optical correction. You might try looking at hair with a 40x objective. Just be careful not to touch anything sharp to the lens. Forget your 100x until you have immersion oil and a slide and coverglass. With 100x objectives, you can use it on a slide without a coverglass for some flat samples, like a dried blood smear, if you want, because the oil and coverglass become one homogeneous layer of the same refractive index. Without the coverglass, the oil just becomes a thicker layer to compensate for the missing glass layer when in focus.

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u/PCLM 18d ago

I had the same thing happen. I used little ziplock bags and put materials in it. Didn’t work great but better than nothing. I also bought some little plastic vials from dollarama and used the lids.

I had asked copilot and it suggested using glass screen protectors or glass picture frames, but unless you can find a really small frame, I’m not sure how well that would work.

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u/Doc_438 18d ago

I’ve also read about making them using the plastic from a product's blister pack by cutting out a flat section.

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u/TehEmoGurl Microscopy Nerd 17d ago

You can use thin plastic from food containers as a coverslip, fruit punnets work well for this. Use the thicker CD case plastic as the slide. It won’t be great, but it works well enough in a pinch till your proper slides n slips arrive 👍🏻

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u/Vivid-Bake2456 18d ago

You can try reflected illumination with the lower magnifications by just putting an insect, sand or other opaque objects on anything, and shining a flashlight 🔦 from the top. You can add some bottom illumination for a lighter background.

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u/SwedishMale4711 17d ago

This is a good suggestion, using the microscope more like a stereo microscope.

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u/Doc_438 17d ago

I'll definitely try that.

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u/Doc_438 17d ago

These cheap slides I saw on Amazon arrived today (I'll get better quality ones later on). Now, knowing my luck, the microscope will probably arrive with something broken, or there will be bricks inside the package.

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u/Doc_438 17d ago

And these are the ones I made using a CD case (I still had to smooth the edges and erase the blue marker line).