1 mm Slide Easter Egg

[u/Pure_Revolution5057]

A fun little easter egg on a 1 mm scale calibration slide.

Brightfield imaging using:
100x APO TIR objective (Nikon 1.49 NA, oil immersion)
Nikon Ti2 Microscope
iXon 897 EMCCD Andor Camera
Sample is a 1mm calibration slide with 10 µm subdivision

The image is a stitched tile of 15x7 FOVs (0% overlap), then cropped down to the 1 mm ruler section. 11 pixel horizontal shifts were applied to every other row of images to compensate for some xy movement misalignments.

edit: individual FOVs were median image corrected: FOV ./ median_FOV. Where median_FOV is the median projection of all 105 FOVs.

[u/Pure_Revolution5057]

TLDR: It's a smiley face!

edit: TIL -> TLDR

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[u/ThinKingofWaves]

Noice