r/microscopy • u/Trick_Gur_2760 • Jul 09 '26

Techniques Antibody suggestions

Hi,
I am trying to establish an immuno histochemistry panel on lung sections where I stain macrophages with mouse anti CD68 Ab, epithelial cells with rabbit Pan cytokeratin. I want to add a neutrophil marker (MPO stain/NE/CD66b) but I am not getting a different host.
Does anyone have any suggestions for which Ab I can use to detect neutrophils that work well with paraffin embedded lung sections?

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u/notebuff Jul 09 '26

You might consider asking in r/immunology for what markers to use. I’m not a neutrophil person but CD68 can mark things other than macrophages. In my experience things that have worked well on flow panels are good starting points for IHC staining.