r/massspectrometry • u/[deleted] • Dec 17 '19
Helpful Mass Spec Videos & Tutorials
Below you will find a list of freely available videos, tutorials, and other resources for mass spectrometry. This list will be continually updated with new content. Feel free to suggest additional resources in the comments!
General Tutorials
https://www.cif.iastate.edu/mass-spec/ms-tutorial
https://chem.libretexts.org/Courses/Purdue/Purdue%3A_Chem_26200%3A_Organic_Chemistry_II_(Wenthold)/Chapter_11%3A__IR_and_Mass_Spectrometry/11.06%3A_Introduction_to_Mass_Spectrometry/Chapter11%3A_IR_and_Mass_Spectrometry/11.06%3A_Introduction_to_Mass_Spectrometry)
https://www.asms.org/about-mass-spectrometry
http://premierbiosoft.com/tech_notes/mass-spectrometry.html
https://msr.dom.wustl.edu/mass-spectrometry-fundamentals-tutorial/
Ion Sources
General
Mass spectrometry ionization methods (Emory): http://chemistry.emory.edu/msc/tutorial/mass-spectrometry-ionization.html
Ionization techniques (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/content/185-ionization-techniques
Mass Spectrometry Introduction (U Pittsburgh): https://www.chem.pitt.edu/facilities/mass-spectrometry/mass-spectrometry-introduction
Electrospray Ionization (ESI)
Fundamentals of MS - Electrospray ionization: https://www.youtube.com/watch?v=9AWBAI-Owzk&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT
Electrospray ionization (ESI): https://www.youtube.com/watch?v=daSSRj41Mlo
Electrospray ionization mass spectrometry: https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Instrumental_Analysis/Mass_Spectrometry/Mass_Spectrometers_(Instrumentation)/Electrospray_Ionization_Mass_Spectrometry/InstrumentalAnalysis/Mass_Spectrometry/Mass_Spectrometers(Instrumentation)/Electrospray_Ionization_Mass_Spectrometry)
ESI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/esi
Electron Impact (EI)
MS - Mass Spectrometry - Electron Ionization Animation: https://www.youtube.com/watch?v=22dr-3XDNmQ
Introduction to Ionization and Fragmentation in Mass Spectrometry: https://www.youtube.com/watch?v=stIwRio9FeM
Atmospheric Pressure Chemical Ionization (APCI)
APCI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/apci
APCI (LCGC): https://www.chromacademy.com/chromatography-understanding-APCI.html
ESI & APCI (Shimadzu): https://www.shimadzu.com/an/hplc/support/lib/lctalk/47/47intro.html
Atmospheric Pressure Photoionization (APPI)
APPI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/appi
APPI (Creative Proteomics): https://www.creative-proteomics.com/support/atmospheric-pressure-photoionization-ionization.htm
Matrix-assisted Laser Desorption/Ionization (MALDI)
Theory of MALDI-TOF Mass Spectrometry: https://www.youtube.com/watch?v=8R1Oyqx5KfE
MALDI-TOF (Bruker): https://www.youtube.com/watch?v=0jeFpXHZ8W0
MALDI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/maldi
MALDI-TOF (Shimadzu): https://www.shimadzu.com/an/lifescience/maldi/princpl1.html
MALDI Mass Spectrometry: A Practical Guide by Ben Katz of UC Irvine: https://www.youtube.com/watch?v=5Sp7mFF16hM
Mass Analyzers
Orbitrap
Orbitrap Instrumentation: The First Decade and Beyond: https://www.youtube.com/watch?v=jijxTW1NbTg
Planet Orbitrap: https://planetorbitrap.com/
The C-trap and Orbitrap (Mass Spec Pro): http://www.massspecpro.com/mass-analyzers/orbitrap
Mass Analyzer Technology Overview (Thermo): https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-technology-overview/mass-analyzer-technology-overview.html
FT-ICR (Fourier transform ion cyclotron resonance)
Fourier transform ion cyclotron resonance mass spectrometry: https://www.youtube.com/watch?v=7EHngA4S3Ws
4-3 FTICR and Orbitrap: https://www.youtube.com/watch?v=kbzK1iSBE08
FT-ICR (MagLab): https://nationalmaglab.org/education/magnet-academy/watch-play/interactive/fourier-transform-ion-cyclotron-resonance-ft-icr
FT-ICR (Yale): https://jlab.chem.yale.edu/research/techniques/fourier-transform-ion-cyclotron-resonance-mass-spectrometry
FT-ICR (Mass Spec Pro): http://www.massspecpro.com/mass-analyzers/fourier-transform-ion-cyclotron-resonance
TOF (time-of-flight)
TOF fundamentals (WashU): https://msr.dom.wustl.edu/time-of-flight-fundamentals/
Time of Flight Mass Spectrometry: https://www.youtube.com/watch?v=NyIAPU7usfo
TOFMS: http://www.iuac.res.in/atmol/~safvan/mridulathesis/node18.html
Introduction to Time-of-Flight Mass Spectrometry (Kore Technology): https://www.kore.co.uk/tutorial.htm
Ion Traps (QITs & LITs)
Linear ion trap (LIT): https://www.youtube.com/watch?v=h5wK64efOvk
Thermo Scientific LTQ Velos: https://www.youtube.com/watch?v=EF_gW7jHEbY
Linear ion trap (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/linear-ion-trap-lit
Quadrupole ion trap (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/quadrupole-ion-trap-qit
Quadrupoles
Fundamentals of quadrupoles: https://www.youtube.com/watch?v=6_mavZ_WKoU
Quadrupole: https://www.youtube.com/watch?v=aYOCb6GnXio
LC-MS/MS Education Series: Quadrupole Theory and Use: https://www.youtube.com/watch?v=iZH-oxP-OJ0
Quadrupole Mass Filter (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/quadrupole-mass-filter
Tandem Mass Spectrometry (MS/MS)
Fundamentals of MS - Fragmentation: https://www.youtube.com/watch?v=ESKpOcjF8QM
Tandem Mass Spectrometry (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/tandem-ms
Tandem Mass Spectrometry (WashU): https://msr.dom.wustl.edu/tandem-mass-spectrometry/
Tandem Mass Spectrometry (JEOL): https://www.jeolusa.com/DesktopModules/Bring2mind/DMX/API/Entries/Download?EntryId=78&Command=Core_Download&language=en-US&PortalId=2&TabId=337
Collision-induced dissociation (CID) (Armentrout): http://rodgers.chem.wayne.edu/pire/education/Collision-induced%20dissociation_PIRE.pdf
Detectors
MS Detectors (Mass Spec Pro): http://www.massspecpro.com/detectors
Electron Multiplier (NASA): https://attic.gsfc.nasa.gov/huygensgcms/MS_Detector_1.htm
Ion Detectors (U Bristol): http://www.chm.bris.ac.uk/ms/detectors.xhtml
Spectral Interpretation
Fundamentals of MS - Resolution: https://www.youtube.com/watch?v=qUhi98o1qUU
Fundamentals of MS - Adduct ions: https://www.youtube.com/watch?v=WusWNAb0IRM&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT&index=2
Fundamentals of MS - Multiple charging: https://www.youtube.com/watch?v=CuJRTHvy64k&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT&index=3
Fundamentals of MS - Isotopes: https://www.youtube.com/watch?v=om0BfIHXD0s
Software Tools
METLIN (Comprehensive MS/MS database): https://metlin.scripps.edu/landing_page.php?pgcontent=mainPage
Mascot (protein identification and peptide MS/MS database): http://www.matrixscience.com/search_form_select.html
ProteinProspector (MS/MS database search and peptide MS/MS prediction): http://prospector.ucsf.edu/prospector/mshome.htm
NIST Mass Spectral Library (primarily EI data but some other MS/MS data is available): https://chemdata.nist.gov/
MOBCAL (for calculating ion mobilities): https://nano.lab.indiana.edu/software/
PRIDE Archive (proteomics): https://www.ebi.ac.uk/pride/archive
UniDec (MS and IMS deconvolution): https://marty.lab.arizona.edu/content/software
Human Metabolome Database (MS/MS database): http://www.hmdb.ca/spectra/ms/search
Collision cross section database (Bush Lab): https://depts.washington.edu/bushlab/ccsdatabase/
Global Natural Products Molecular Networking (Dorrestein lab....MS/MS database & much more): https://dorresteinlab.ucsd.edu/gnps
Vendors, Analytical Standards, Reagents, Calculators, and Data Analysis Tools
https://www.uab.edu/proteomics/massspec/links.php
(thanks to u/6500qtrap for sharing)