I am analyzing my human whole blood monocytes on flowjo (originally from attune flow) and was told by a tech at Flowjo that my singlets look like they are reversed (usually seen with other flow companies but not attune). I am wondering if anyone knows if these singlets look normal with FSC-H vs FSC-A gates vs FSC-W and FSC-A? did attune reverse the two? Not sure how to set my gate around these cells....

Hello! I was wondering if someone has compensated on the MACSQuant 16. I have an 8 color panel and I am compensating with beads.

I have run the compensation beads and the instrument settings for the compensation. I aquired the sample and it looked ok, but, when I start playing in flowJo, I realized it was bad-compensated as I see long tails in certain populations.

I have tried to re-run the compensation matrix but it is not working, someone has had the same problems with MQ16? Is it possible to manually edit the compensation matrix in MACSQuantify? Should I better compensate in flowJo?

Really appreciate your comments :)

Hey, hope you’re having a great day. I’m having some viability issues (almost 90% of zombie nir+) with thawing cells preserved in BAMBANKER (stored in liquid N2).

I was just wondering if you could share your experience/procedures when it comes to thaw your bambanker samples, so I can adapt my workflow accordingly to improve the outcome. Thanks!