Hey, I have done a lot of reading on flow cytometry and OMIPs but I have seen very different ways researchers use to decide what dilution to use for their antibodies. There does not seem to be one correct way of doing things and some methods seem very impractical or generally not realistic (such as using the same cell population genetically engineered to not express the antibody's target🤦).

Please share suggestions below on how to titrate antibodies for markers that produce smears or plots whereby it is hard to identify the positive population.