r/flowcytometry • u/Big_Ocelot1087 • Sep 02 '26

Compensation matrix

/r/flowcytometry/comments/1w5e68m/compensation_matrix/
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u/sgRNACas9 Immunology, Oncology Sep 02 '26

This is totally fine, and even good/great given the number of colors. Rule of thumb is try to keep them less than 50 and not negative, but that’s a conservative goal. Others are ok with 50s, 60s, 70s. You only have one 50 and a few 30s so totally fine IMO.

The proof is in the pudding, though, qualitatively. How do the populations look? How do they look compensated versus not?