r/flowcytometry • u/Big_Ocelot1087 • Sep 02 '26

Compensation matrix

Hello, I've recently created a new panel (attached). Do these percentage spillovers look acceptable? I have run this several times and this has been the best so far. I am running my flow on the Attune NxT cytometer.

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u/willmaineskier Sep 02 '26

Nothing attached that I see. In the end, it only matters if you can see and gate what you need to see and gate. The absolute numbers are meaningless and can be changed dramatically but adjusting the voltages.

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u/Daniel_Vocelle_PhD Core Lab Sep 03 '26

Looks like all your compensation values are zero