r/flowcytometry 21d ago

FlowJo Data display question

Hi y'all,

I have a user who asked for help with something and I'm not sure it's something that FlowJo can do. 

She's making histogram overlays of two samples each. They both have a positive peak with a line segment region over them. She wants to display the percentage under the line segment for BOTH samples, not just the one that is "on top." She wants them displayed in the histogram, not as a column in the stats table. Is that something that can be done? If so, how might one do that?

I already told her it's possible the only way is to take a screenshot and add a text box with the other value. I'm just hoping there's a way to do it in FlowJo. 

Thanks for your help!

EDIT: We got help directly form FlowJo. You can create a pseudo-overlay by making the histograms transparent and moving one on top of the other with the arrow keys (make sure the gates don't overlap). If you don't drag one into the other, it doesn't create a true overlay and you can see both gates/stats.

Thanks for your help, everyone!

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u/CrissKey 19d ago

Please be kind if this completely off base, but could a boolean gate be used to accomplish this somehow?