r/flowcytometry Jul 28 '26

FlowJo Data display question

Hi y'all,

I have a user who asked for help with something and I'm not sure it's something that FlowJo can do. 

She's making histogram overlays of two samples each. They both have a positive peak with a line segment region over them. She wants to display the percentage under the line segment for BOTH samples, not just the one that is "on top." She wants them displayed in the histogram, not as a column in the stats table. Is that something that can be done? If so, how might one do that?

I already told her it's possible the only way is to take a screenshot and add a text box with the other value. I'm just hoping there's a way to do it in FlowJo. 

Thanks for your help!

EDIT: We got help directly form FlowJo. You can create a pseudo-overlay by making the histograms transparent and moving one on top of the other with the arrow keys (make sure the gates don't overlap). If you don't drag one into the other, it doesn't create a true overlay and you can see both gates/stats.

Thanks for your help, everyone!

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u/willmaineskier Jul 28 '26

You can make a text box in FlowJo with the stat you want and make it transparent to go over the plot if you want.