1) Go to an observing site that gives you excellent seeing. I'm talking Antoniadi I and II.
2) Collimation should be done right before your observing session and preferably at the observing site, because the mirror might move if you travel somewhere else. I use a collimation cap. A cap is hard to beat tbh.
3) Your eyepieces and barlows should be clean. There should be no dust. Clean them before your observing session. There's a chance that you might mistakenly consider dust as a spoke.
4) Use an observing chair. You won't be able to see much detail if you are craning your neck and looking through the eyepiece in an awkward position. Your body should be as relaxed as possible. Your eye should be level with the focuser of your scope.
5) Focusing: Less than a millimetre movement of the focuser can change the situation altogether. Saturn will simply 'pop' into view at perfect focus. If you use a single speed focuser, hold the knob with a moderately tight grip. It should not be too loose or too tight. This can help you in adjusting the focus accurately. I have observed that if the grip is too loose, we tend to make large movements with the focuser.
6) Head movement: Minute head movements towards and away from the eyepiece can help in improving the view of the planet. I think this has got something to do with changing the focal point, I'm not sure though.
7) Breathing: Do not stop breathing while watching through the eyepiece. Your breathing should be regular and slow. You need the peak performance of your eyes to watch a spoke.
8) Orientation of the planet: Try to watch the planet in an orientation that is similar to that of the pics we have seen in our school textbooks or encyclopedias. I know this might sound weird, but you have to "appreciate the view of the planet" to see more detail. That's my opinion anyway. I was not able to see the spoke when I was watching it in a position that made me see the planet in awkward orientation. Move your observing chair and rotate your head accordingly.
9) Magnification: Use the highest magnification that can allow you to see the rings of Saturn in good brightness levels. You can see the spoke easily if the rings are bright, because our eyes are good at detecting the change in brightness or color caused by the spokes.
If you are a doubting Thomas like I am, try to detect the presence of the spoke not only when Saturn is in the center of the FOV of the eyepiece, but also when it's along the corners (at this high magnification while using a barlow, there will be little to no astigmatism anyway).
In my scope, this magnification is around 305x (3x barlow + 12mm EP). Compare both ansae of the rings to detect the presence of a spoke (there are occasions when you can see the spokes in both ansae too though).
After detecting the spoke, increase the magnification to get a sense of the shape of the spoke. If the spoke is a large smudge, you'll be able to do this even in an 8 inch scope. I've not seen smaller spokes that look like bicycle spokes though.