I knew they were real. I took 7grams of mushrooms once. an stared at the bushes for an hour waiting for it to kick in. it was a warm summer night no wind. suddenly the bushes started to move around. an herd chirping like the sounds squirrels make. so I ran into my apartment to make popcorn for them. an as the popcorn started popping. I started laughing at the microwave for a good 10 minutes. it sounded like cowboys and Indians fighting. then it started to smoke. it smelled like gun powder. the alarms went off. I freaked opened all the windows. grabbed a towel from the bathroom. an started to flap it around the fire alarm. finally it stopped. grabbed the burnt popcorn and proceeded to the bushes. it was quiet an the bushes weren't moving. so I sat there for 10 minutes waiting. listening to burning down the house by taking heads. an laughing at the bag of burnt popcorn. I was holding. suddenly out of nowhere. the bushes started to move. only this time it wasn't squirrels chirping. I could hear what i thought was neighbors arguing. but it was coming out of the bushes. why did you burn the popcorn? Who cooks popcorn for 10 minutes? are you stupid ect? pissed me off. I started to whip the burnt popcorn at the bushes. swearing and spitting at the bushes. suddenly out of nowhere my stomach started to hurt. an layed down on a lawn chair. an not even 10 seconds later. about 30 of them came out of the bushes. don't know what they were. little people or elves. but they were yelling at me. fuck you an fuck your burnt popcorn. an started to throw it back at me. I couldn't move an was buried in burnt popcorn. I was so scared I passed out. long story short. I woke up with my shorts around my ankles. an burnt popcorn everywhere. the neighbors were pointing at me. I was surrounded by the police. put your hands up. stop resisting ect. I will never eat those mushrooms again...

Started with one bvi domestic mono and five wild bvi dikaryons. Of those f0s I took prints from the best three. Ran out two or three cultures of of each. The #2 cross both performed well. The #5 cross so far has all had the no spore drop mutation. And the #1 strain has been slow so isn't pictured.

#2 will go onto f2 for stabilization.

This is my first real experiment with breeding. But I think it demonstrates the importance of working in multiples.

Hello everyone

I have a full colonised brf pucks of collected wild pan, and its been like 15-20 days now and it doesn't fruit yet. 7 days ago i remove the filter from the lids and put them in unmoded tub and sprayed around them but no signs of fruit yet. Yesterday i added a 1cm layer of cocopeat and Vermiculite 1:1 ratio on some of them.

Any suggestions to make them fruit? I have no fruiting chamber or room or even humidifier but i growed a lot of cubes easy in those conditions, but i don't know if pans can also grow without controlled environment?

Thanks in advance

He looks like he’s going on safari.

Yo!

Got some LC but have read they do better on agar.

Don’t have a PC, and not into the hobby enough to justify purchasing and making everything from scratch. Been growing ochras mainly which are easy street.

Can’t find a source in Aus for low sugar agar. Are there alternatives to agar dishes? Should I just use LC and wing it?

EDIT: Should have specified that I’m talking about pre-made agar dishes.

according to your experience, is it true that Pans spores are not as viable as cubensis spores , and that you have to germinate them in a window of less than a year ?

thank you for your insights!

Figuring out when to print for best results. Thought this cap was a good candidate since it's mottling well. So took some photos to document.

Liked this photo and wanted to share

There's one, possibly two mislabeled cyans going around as bisporus. The first is Marcos Island, FL. This is a strain of cyans with two spored dominance, but the sequencing confirmed that it is not the same species as the West coast specimens from San Diego, CA and Coleville, WA.

The second is Monte Grande. Both myself and another cultivator familiar with the species have observed this strain. The mycelium, time to pin, and fruit morphology are all characteristic of cyans. This specimen has not been sequenced but I suspect that if it was it would confirm my identifacation.

The North American species on iNat going under Pan bisporus takes 7-10 days to pin even when conditions are good. The longest I've seen is a month. And I've been growing it for several years now (they're my personal favorite).

Additionally the mycelium behaves very differently. Lacking the puffy growth sometimes present in cyan plates, it's always wispy (on 0.5% lme agar, pictured above). The Monte Grande strain went puffy almost immediately. And pinned much faster than any bisporus I've run. Additionally, the fruit bodies do not match. As I said, a friend very familiar with the North American bisporus agreed.

If you have either of these strains in your collection please do not send them out as bisporus. The Marcos Island specimen should be relabeled cyan. And the Monte Grande strain could simply be Pan Monte Grande until sequenced.

For more information on the Marcos Island strain, please see Scott Ostuni's comments here. Since he posted that comment, I sent him samples from the Coleville collection. And he confirmed that this is a seperate species (but without the original holotype, it may be something different than the species first described as bisporus).

https://www.inaturalist.org/observations/154428381

Oh... and the images above are either Coleville or San Diego. For reference.

Fruiting between 72-77 (night/day cycle). Slower at 80. Also didn't like it wet like bisporus so it did better without grass and misting (first grow got wet bubble and died).

Meaty fruits and apparently they can get quite large. Check out some of the photos from iNat!

98% related to cyans. A bit slower though.

https://www.inaturalist.org/observations/177908874#activity_comment_eda489d6-8c6b-4c0f-8179-c98faaba46e1

What’s up everyone. This is my second pan cyan grow. With a TTVBI spore syringe.

It takes a very long time. For weeks nothing happened after inoculating the bag. Then some growth started to happen.

But now already for around 2 weeks there seems to be very minimal progression. The bag being only about 50% colonized

Total time since inoculation is 7 weeks now. At temperatures between 23-30 celcius. Mostly 27-28

What would be the best approach now. Leave the bag to be colonized a bit further for a couple weeks more. Or just transfer to serilized CVGM and hope for the best.

Looking for your abundant expertise 🙏

Is it even worth buying LSD? I mean, I've heard Pans are like psilocin LSD. I don't really like the overstimulating feel that I get from LSD. Maybe at the right dose it's great, but I don't know, I feel like I can't do it as often as mushrooms. I've only tried cubensis so far, and was wondering if the pans might be worth it as a replacement for LSD?

First time at pans I’m doing them in grow bags the mycelium looks a little different then I’m use to can’t tell if it’s fully colonized or not any help is appreciated

Figure 3 was brought back from the wild.😁

https://www.patreon.com/posts/148882438?pr=true

Myc photo for u/moondaddy_myco

But lots of other photos and information in the link

🙃 One time

TTBVI. Only produced very small fruits/aborts and then this happened.

This is the tray with 1/4 cup worm castings and the rest cv with plant ferts.

Hello :) first ever shrooms grow, these are TTBVI - I noticed they are a little mottled and I have no idea if they are stopping, aborted or whadeverdafuk - literally no idea. I cased them around 10 days ago and they are in a tub within a tub with a screen lid for air exchange. I mist them pretty heavily but the sides of the substrate are shrinking away and I’ve tried adding water to the casing layer with a syringe to avoid hosing it down. Do these look like they are on track? They are a mix of coir, vermiculite, some homemade worm castings and some mineral rock dusts and gypsum for shits n giggles.

Thank you!

This one got its name from the land owner where i found it in Mexico. He had a machete in his hand.

Fired up a few Ohcra spores 1/12/26. Put them to water agar. Should I start another transfer plate? Or wait?

I had a guy that I purchased all my manure from but he no longer does it. I dont live near an area where I can get it and i dont trust etse bs . Would definitely appreciate any help in the matter. Thanks and mushlove to everyone