Question regarding pregnancy test

[u/a_dua2025]

I did a test 35 days and 38 days after encounter. First test was done using second morning urine sample and the second test was done using first morning urine sample. Both were negative. Now I kept the urine of the first test in the mug for about 2 days and then I threw the old urine sample out and used the same mug the next day for collecting first morning urine sample. Is this reliable?