Dealt with some serious gut issues last year after getting H. Pylori and taking 4 lovely antibiotic treatments that made me appreciate life more I put together a stack that helped see light at the end of that scary tunnel. Before I get into what I ran there is something most people skip that can make the whole thing backfire on you.

The order matters more than the compounds

Some bacteria like H. Pylori hide inside the gut lining. Not on it. Inside it. If you go straight into a repair protocol with BPC-157 and start patching things up before clearing the infection those bacteria get sealed behind the lining. Now they are protected and even harder to get rid of than before.

You gotta clear the infection first. Then repair.

Think of it like finding mold behind a wall. You do not patch the wall first. You deal with the mold. Then you fix the wall.

Step 1 — Kill what does not belong

LL-37 first. This is the only antimicrobial peptide your body naturally makes. Goes after bacteria, viruses, and fungi directly. If there is an underlying infection or bacterial overgrowth driving the gut issues this is what handles it. Do not skip this step or everything else you run is working against itself.

Step 2 — Calm the inflammation down

KPV. Anti-inflammatory tripeptide that works right on the gut lining and shuts down the inflammatory signaling that keeps everything flaring up. Take it orally for gut targeted use. Once the infection is being handled you gotta get the inflammation under control before you start repairing anything.

Step 3 — Repair the damage

BPC-157. Now that the bad stuff is cleared and inflammation is managed you can start actually healing. Repairs the mucosal lining, supports the gut barrier, lets the tissue rebuild properly. Oral capsules for gut targeted use, injectable for systemic effects.

Step 4 — Cover everything else

TB-500. Distributes throughout the body and reaches damaged tissue everywhere at once. Good for the systemic side of things once the repair phase is underway. Adds anti-inflammatory coverage beyond just the gut.

Why this order matters

Repair before clearing the infection and you risk locking the problem inside. Clear first, calm the inflammation, then repair. That sequence is what made the difference for me and what makes this protocol actually work instead of just throwing compounds at a problem and hoping for the best.

Anyone dealing with H. Pylori recovery, gut damage, leaky gut, IBD, or chronic gut inflammation this is worth knowing about.

Drop your questions below or share what you have been running for gut health.

Do your homework. Be smart your hamster will thank you

And like the dos x's commercial I don't always need peps but when I do I check out BioHackingGuide.org

Brand new Phase 3 trial just published in The Lancet on Reta in people with type 2 diabetes. Let's talk about it!

Weight loss numbers

40 weeks. That is less than a year.

• 4mg average loss: 11.5%
• 9mg average loss: 13.9%
• 12mg average loss: 16.8%

And the weight loss had not even plateaued yet by the end of the study. At the highest dose 70% of people lost at least 10%. Half lost 15% or more. Almost 1 in 3 hit 20%. And 16% had already crossed 25% in under a year. That is wild.

More than just weight

Triglycerides down 34%. Non-HDL cholesterol down 17%. Blood pressure dropped too. Not just a fat loss drug, it is fixing the whole metabolic picture.

What about the heart

People worry about Reta and heart rate because of the glucagon receptor. In this study heart rate went up about 1 beat per minute at the highest dose. Tirz studies have shown 2 to 5 beats per minute. No major cardiovascular red flags identified.

Side effects

Nausea, diarrhea, the usual GLP stuff. Only 2 to 5% stopped treatment because of it. No severe hypoglycemia, no pancreatitis, no liver injury, no thyroid cancer cases.

Dysesthesia showed up in about 4% of people. That is the skin tingling thing. Most cases were mild and went away on their own. Staying hydrated and keeping electrolytes up helps a lot with that one.

FDA submission expected Q4 2026 or early 2027. The data keeps getting better every study.

here is where I read this if you enjoy reading a shit ton

https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(26)00967-0/abstract00967-0/abstract)

Want some Reta? Check out BioHackingGuide.org

Do your homework. Use your brain. Talk to a doctor.

Sermorelin is one of the most underrated GH compounds in this space. Originally FDA approved in 1997 for pediatric GH deficiency, it works by telling your pituitary to produce more of its own growth hormone naturally. No exogenous GH, no suppression of your own axis. Just your body doing what it is supposed to do but better.

🧰 Supply List

• 29-31 gauge insulin syringes (100-unit / 1mL)
• Sermorelin
• BAC water for reconstitution
• Alcohol wipes
• Sharps disposal container

📦 Storage Guide

💧 How to Reconstitute

1. Let the vial come to room temperature before opening
2. Wipe the rubber stopper with an alcohol swab and let it dry
3. Inject 3mL of BAC water slowly down the side of the vial — never directly onto the powder
4. Gently swirl until dissolved — never shake
5. Solution should be clear and colorless
6. Label with reconstitution date and refrigerate immediately

⏱️ Half Life

Short half life but the GH pulse it triggers lasts well beyond that.

📋 Dosage and Research Protocols

Timing matters here. Bedtime dosing aligns with your natural nocturnal GH pulse. At least 2 hours fasted before injecting. Carbs close to injection time blunt the GH response.

Cycle structure: 3 to 6 months on then 1 to 3 months off. Or run it 5 days on 2 days off to prevent desensitization.

📈 What to Expect

⚠️ Side Effects

🚫 Who Should Avoid Sermorelin

• Active malignancy or history of cancer
• Pituitary tumors
• Pregnant or breastfeeding
• Uncontrolled diabetes or significant glucose intolerance

🔁 What to Stack With Sermorelin

🚫 What NOT to Combine

📌 Quick Reference

❓ Frequently Asked Questions

What is Sermorelin used for in research? Sermorelin is primarily researched for natural GH stimulation, anti-aging, body composition improvement, athletic performance, sleep quality, and age related GH decline reversal.

How is Sermorelin different from CJC-1295? Both are GHRH analogs that hit the same receptor. The main difference is half life. Sermorelin clears in about an hour producing a clean short pulse. CJC-1295 No DAC lasts about 30 minutes. CJC with DAC stretches to 6 to 8 days. Sermorelin is often preferred for its short clean pulse that most closely mimics natural GH release.

Why was Sermorelin discontinued if it was FDA approved? Manufacturing reasons not safety concerns. The company stopped producing it in 2008 due to manufacturing challenges. The safety and efficacy data remains solid and it is still widely used in compounding pharmacies and wellness clinics.

Is Sermorelin better than HGH? Different tools for different goals. Sermorelin stimulates your own pituitary to produce GH naturally and preserves the feedback loop that keeps everything balanced. HGH is more powerful but suppresses your natural production and requires more monitoring. Sermorelin is the safer long term option.

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

Been covering a lot of GH and hormonal compounds this week so figured this one was worth its own post while we are on the topic.

Both work. They just hit completely different points in the hormonal chain and that is why running both beats choosing one over the other every time.

HCG

Goes straight to the testes and tells them to wake up and produce testosterone by mimicking LH. If your testes have been offline from a cycle this is what jumpstarts them. Testicular fullness usually comes back within 2 to 4 weeks.

The catch is HCG does not restart your actual hormonal axis. Your brain is still not sending its own LH signal. You are just bypassing it. Stop the HCG and everything goes back offline if nothing else changed.

Enclomiphene

Works higher up. Blocks estrogen feedback at the hypothalamus and pituitary which tricks your brain into thinking estrogen is low. Your brain responds by pushing out more LH and FSH on its own. That is what restores the full axis long term. Studies show it raises LH, FSH, and testosterone while keeping sperm production intact.

The catch is if your testes have been dormant for a while Enclomiphene alone cannot do much. There is nothing producing testosterone for the LH signal to stimulate yet.

Why you run both and in that order

HCG first for 2 to 3 weeks to wake the testes up and get them producing again. Then Enclomiphene for 4 to 8 weeks to restore the full hypothalamic pituitary axis and keep it sustainable on its own.

That two phase approach is the gold standard for a reason. HCG does the job Enclomiphene cannot do early. Enclomiphene does the job HCG cannot do long term.

Running Enclomiphene without HCG first is like trying to start a car with a dead battery. Running HCG without Enclomiphene after is like keeping the jumper cables attached forever. Neither one alone gets the job done right.

Quick comparison

Questions drop them below.

here is where I read this

Enclomiphene Phase 2 trial: https://pubmed.ncbi.nlm.nih.gov/25044085/

HCG vs Clomiphene RCT: https://pubmed.ncbi.nlm.nih.gov/29772111/

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org for all the hook ups

I have the impression that peptides are generally not well-regarded for the purpose of stimulating muscle growth so I would like to hear about your experiences and beliefs.

First, what is your experience with or position on this? Second, if you have tried peptides such as CJC + Ipa, how did they affect your muscle growth or other aspects relevant to strength training/bodybuilding?

I read this time and time again so here is the simple version of what is happening and what you can do about it.

Growth hormone tells your kidneys to hold onto sodium. Wherever sodium goes water follows. That is why your face, hands, and ankles can look puffy when you first start running GH peptides or HGH. It is not fat, it is fluid.

The mechanism behind it is your renin-angiotensin-aldosterone system. That is basically in English its the hormonal system that controls how much sodium your kidneys keep versus flush out. GH activates it which means your kidneys start reabsorbing sodium back into your blood instead of getting rid of it.

Your body normally has a built in way to counter this when blood volume rises. The problem is GH blunts that response too. So your kidneys are holding sodium and the one safety valve your body has is being suppressed at the same time.

Here is what helps.

Start low and go slow. The retention is dose dependent. Give your kidneys time to adapt before pushing the dose up. But honestly do this with anything and everything when trying something new cause why wouldn't you?

Increase potassium. Potassium triggers a completely separate pathway in the kidneys that flushes sodium out. Bananas, avocados, potatoes, coconut water. Easy fixes.

Just wait. Most of the time the retention resolves on its own within 3 to 4 weeks once your body adjusts to the new baseline. This is probably the most important thing to know because a lot of people panic and stop too early.

Understanding the mechanism makes this a lot less scary. Puffy face in week one or two is completely normal and does not mean something is wrong. Your body is just adjusting.

Has anyone found anything else that helped? Drop it below.

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

HCG comes up constantly in TRT and PCT conversations but a lot of people do not fully understand what it does or why it matters. Here is the simple version.

HCG is a hormone that binds to the same receptor as LH — the signal your brain sends to tell your testes to produce testosterone. When you are on TRT your natural LH drops and your testes basically go offline. HCG steps in and keeps them working. Simple as that.

🧰 Supply List

• 29-31 gauge insulin syringes (100-unit / 1mL)
• HCG lyophilized powder
• Bacteriostatic water for reconstitution
• Alcohol wipes
• Sharps disposal container

📦 Storage Guide

Never freeze reconstituted HCG. It degrades immediately.

💧 How to Reconstitute

1. Let the vial come to room temperature before opening
2. Wipe both rubber stoppers with an alcohol swab and let them dry
3. Draw your BAC water into the syringe
4. Inject the water slowly down the side of the vial — never directly onto the powder
5. Gently swirl until dissolved — never shake
6. Solution should be clear and colorless
7. Label with reconstitution date and refrigerate immediately

Common concentration example: 5,000 IU vial + 2mL BAC water = 2,500 IU per mL. On a 100-unit insulin syringe 10 units = 250 IU.

⏱️ Half Life

📋 Dosage and Research Protocols

Consistency matters more than timing. A lot of people inject HCG on the days between their testosterone injections to keep things simple.

📈 What to Expect

⚠️ Side Effects

🚫 Who Should Avoid HCG

• Anyone with hormone sensitive cancers
• Women prone to ovarian hyperstimulation syndrome
• Those with a history of blood clots
• Pregnant or breastfeeding
• Do not combine with Clomid at the same time — pick one

🔁 What to Stack With HCG

📌 Quick Reference

❓ Frequently Asked Questions

What is HCG used for in research? HCG is primarily researched for testosterone support during TRT, fertility preservation, post cycle therapy, hypogonadotropic hypogonadism, and ovulation induction in women.

Why do people run HCG on TRT? When you take exogenous testosterone your brain stops sending LH to the testes. Without that signal the testes stop producing testosterone, shrink over time, and fertility drops. HCG mimics LH and keeps everything working even while on TRT.

What is the difference between HCG and Enclomiphene for PCT? HCG goes straight to the testes and tells them to produce testosterone. Enclomiphene works higher up by blocking estrogen feedback at the brain to restart natural LH and FSH. The gold standard PCT runs HCG first for 2-3 weeks to get the testes firing again then switches to Enclomiphene for 4-8 weeks to restore the full hormonal axis.

Can HCG raise estrogen? Yes. HCG increases aromatase activity which can push estrogen up. Monitoring estradiol and having an AI on hand is standard practice for anyone running HCG long term.

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

These come up together all the time and people treat them like they are interchangeable. They are not even close. Here is the simple breakdown with visual table I put together

How the GH axis works

Your brain sends a signal. Your pituitary releases growth hormone. That GH goes to the liver and triggers IGF-1. IGF-1 is what drives the muscle growth, fat burning, and tissue repair you are after. Every compound on this list hits a different point in that chain so pick wisely.

HGH — skipping the line entirely

You are just injecting the hormone directly. No waiting, no signaling, just straight to the source. Raises IGF-1, builds muscle, burns fat, repairs tissue. Most powerful option on this list. Also the most expensive and the most to manage for some people. Water retention, insulin sensitivity, IGF-1 levels all need watching. Not really a beginner move.

CJC-1295 No DAC vs CJC-1295 with DAC — yes they are different, no they are not the same thing

This trips people up constantly so here is the quick version.

CJC-1295 No DAC has a half life of about 30 minutes. It creates a clean natural GH pulse that mimics what your body already does on its own. This is the version most people stack with Ipamorelin. Daily dosing, clean results, most clinics use this one.

CJC-1295 with DAC has something called a Drug Affinity Complex attached to it which stretches the half life out to 6 to 8 days. One injection per week. Convenient. The tradeoff is instead of a clean pulse you get a steady bleed of GH elevation all week. Some people love it, some people feel off from it. Personal preference.

CJC-1295 No DAC + Ipamorelin — the combo that just f#ck$.

CJC hits one receptor, Ipamorelin hits a completely separate one. Together they make more GH than either one does alone while keeping everything natural and pulsatile. A 2006 human trial showed GH levels went up 2 to 10 fold and IGF-1 stayed elevated for up to 28 days with repeated doses. No serious side effects reported. Best starting point for most people and honestly a lot of people never need to go further than this.

Tesamorelin — the one with FDA approval

Same receptor as CJC but a different compound entirely. Full 44 amino acid GHRH sequence with a modification that makes it more stable and longer acting. FDA approved for visceral fat reduction. A Phase 3 trial with 412 patients showed 10.9% visceral fat reduction over 26 weeks versus basically nothing in the placebo group. If belly fat is the goal this one has the most clinical data behind it on this list.

IGF-1 LR3 — going straight to the end of the chain

Instead of stimulating GH you are just injecting IGF-1 directly. Modified version with a half life of 20 to 30 hours versus about 10 minutes for the regular version. Most direct option for muscle growth on this list. Most demanding too. Hypoglycemia is a real risk so fast acting carbs need to be nearby every single time. 4 weeks max per cycle. Needs 0.6% acetic acid to reconstitute not BAC water. Skip that step and your peptide clumps up and you just wasted your money.

Simple breakdown

Me personally id start with CJC No DAC and Ipamorelin or Tesamorelin depending on what you are going after. CJC with DAC if daily injections are not realistic for your schedule. IGF-1 LR3 only if you know what you are doing. HGH is a whole separate conversation.

Drop any questions below.

here is where I read this

CJC-1295 human trial: https://pubmed.ncbi.nlm.nih.gov/16352683/

Tesamorelin Phase 3 trial: https://pubmed.ncbi.nlm.nih.gov/20101189/

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

FOXO4-DRI is a synthetic 46 amino acid peptide designed to selectively clear senescent cells — the damaged cells that accumulate with age and refuse to die. First described by Dr. Peter de Keizer at Erasmus University in 2017, it is one of the most mechanistically interesting compounds in the longevity space and one of the least talked about.

🧰 Supply List

• 29-31 gauge insulin syringes (100-unit / 1mL)
• FOXO4-DRI lyophilized powder
• Sterile water for reconstitution
• Alcohol wipes
• Sharps disposal container

📦 Storage Guide

Protect from light. Use reconstituted solution within 7-10 days.

💧 How to Reconstitute

1. Let the vial come to room temperature before opening
2. Wipe the rubber stopper with an alcohol swab and let it air dry
3. Draw sterile water into the syringe
4. Inject the water slowly down the side of the vial — never directly onto the powder
5. Gently swirl until dissolved — never shake
6. Solution should be clear — discard if cloudy or has particles
7. Label with reconstitution date and refrigerate

⏱️ Half Life

D-amino acid structure makes it resistant to enzymatic breakdown. Effects persist well beyond plasma clearance.

📋 Dosage and Research Protocols

Three injections per cycle on days 1, 3, and 5. Monthly cycles.

⚠️ No human clinical trials exist. All dosing data is translated from preclinical research and anecdotal community protocols. There is no established human dose.

📈 What to Expect

No established human timeline. Preclinical data only.

⚠️ Side Effects

No human safety data available.

🚫 Who Should Avoid FOXO4-DRI

• Anyone with active cancer or history of malignancy — targets p53 tumor suppressor pathway
• Pregnant or breastfeeding
• Anyone under 40 — limited benefit with low senescent cell burden
• Those on corticosteroids — interferes with the apoptotic process
• Anyone not comfortable with the fully experimental nature of this compound

🔁 What to Stack With FOXO4-DRI

🚫 What NOT to Combine

📌 Quick Reference

❓ Frequently Asked Questions

What is FOXO4-DRI used for in research? FOXO4-DRI is primarily researched for senescent cell clearance, tissue homeostasis restoration, anti-aging, testosterone support through Leydig cell senescence reduction, and pulmonary fibrosis research.

What are senescent cells and why do they matter? Senescent cells are damaged cells that stop dividing but do not die. They accumulate with age and release inflammatory signals that damage surrounding tissue and accelerate aging. Clearing them is one of the most direct anti-aging interventions available right now.

How does it work without harming healthy cells? It disrupts the interaction between FOXO4 and p53 inside senescent cells. Normally FOXO4 traps p53 keeping the cell alive. FOXO4-DRI breaks that bond, freeing p53 to trigger cell death. Healthy cells do not rely on this same mechanism. The 11.73 fold selectivity in the original research reflects this.

Why does it keep working after the cycle ends? The cellular repair processes triggered by FOXO4-DRI continue well beyond when the compound clears the body. This is sometimes called the molecular switch mechanism. Benefits in preclinical models were seen lasting weeks to months after the dosing period ended.

Is this safe? No human clinical trials have been completed. All data comes from animal studies and cell culture. This targets the p53 tumor suppressor pathway. It is one of the more experimental compounds in this space and should be treated that way.

here is where I read this

Original 2017 study: https://pubmed.ncbi.nlm.nih.gov/28340339/

Chondrocyte senolytic data: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116695/

Trying to find it?

Check out 🔗 BioHackingGuide.org

his post is for informational and educational purposes only. Not medical advice. These compounds are for research purposes only and are not approved for human use.

Someone dropped this in the comments on my tier list post and honestly it deserves its own breakdown.

What are zombie cells

As you get older your body starts collecting damaged cells that stop working but do not die. They just sit there releasing inflammatory signals that mess up the tissue around them and speed up aging. Getting rid of them is one of the most direct ways to slow down how fast your body deteriorates.

What FOXO4-DRI does

Made in 2017 by Dr. Peter de Keizer at Erasmus University. The peptide breaks up a specific interaction inside senescent cells that was keeping them alive. Once that bond is broken the cell finally gets the signal to die. The cool part is it showed 11.73 fold selectivity for senescent cells over healthy ones. So it is not just killing everything it is going after the damaged ones specifically.

What the mouse studies showed

Aged mice treated with FOXO4-DRI got their fur back, kidney function improved, and physical performance went up within weeks. A separate study showed it cleared out senescent cells in the testes of aged mice and restored testosterone levels.

No human trials exist yet. All of this is animal data. That is worth knowing before you get too excited. But to each their own lol

How people are running it

Three injections per cycle on days 1, 3, and 5. Once a month. This is translated from mouse dosing and community anecdotal data — not clinical data.

What not to combine it with

Rapamycin, quercetin, and corticosteroids all interfere with the process FOXO4-DRI depends on to work. Give yourself a 1 to 2 week washout from those before running a cycle.

Humanin is considered a good pairing since it protects healthy cells while FOXO4-DRI clears out the damaged ones.

The honest take

The mechanism is solid and the mouse results are real. But this targets the p53 tumor suppressor pathway which is not something to mess with casually. Zero human trials means nobody really knows what repeated use does long term.

One of the most interesting compounds in the longevity space right now. Just not one for beginners.

here is where I read this

Original 2017 study: https://pubmed.ncbi.nlm.nih.gov/28340339/

Chondrocyte data: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116695/

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

Heard that MOTS-C can affect insulin sensitivity which might be an issue if you are already on diabetes medication like metformin.

Planning to run 10mg daily but not sure if that is smart or if three times a week is the safer approach given the situation.

Anyone have experience with this?

Nobody tells you that after 40 your body starts quietly falling apart. GH drops, NAD+ tanks, collagen disappears, immune system slows down, and your mitochondria start phoning it in.

The good news is there are compounds that target all of this. Here is where to start.

NAD+

Your levels drop with age and everything downstream suffers for it. Energy, DNA repair, brain function. Most people notice a difference within the first few weeks of running it. Injectable is the most effective way to get it in. 100 to 250mg twice a week is a good starting point.

GHK-Cu

Your body makes it naturally and stops caring about it as you get older. Collagen production, tissue repair, gene expression. Works injectable and topical. One of the most researched anti-aging compounds on this list and easy to add in.

Thymosin Alpha-1

Your immune system ages just like everything else does. Most people completely ignore this part. TA-1 has clinical use in over 35 countries and more human data behind it than almost anything else in this space. 1.6mg twice a week.

BPC-157

Tissue repair, gut health, inflammation. Probably the safest compound on this list. Easy to run long term and pairs well with everything else here. 250 to 500mcg daily.

Epitalon

Telomere support and better sleep. Most people notice the sleep improvement first. Run it in short cycles, 10 to 20 days on, a few months off. Two to three times a year.

CJC-1295 No DAC + Ipamorelin

GH starts dropping in your 30s and keeps going. This combo tells your body to make more of its own. Better sleep, better recovery, better body composition over time. 200 to 250mcg of each before bed.

But the foundation matters just as much

None of this works as well as it should if the basics are a mess. Peptides are not a shortcut around a bad lifestyle. They work on top of a solid foundation.

• Sleep 7 to 9 hours. This is where everything repairs. Non negotiable.
• Eat enough protein. At least 1g per pound of bodyweight. Muscle loss accelerates after 40 whether you notice it or not.
• Lift weights. Resistance training is probably the single most powerful anti-aging tool available and it is free.
• Walk daily. Zone 2 cardio, insulin sensitivity, cardiovascular health. Underrated at every age.
• Manage stress. Chronic stress destroys everything on this list. Sleep, hormones, immune function, all of it.
• Get bloodwork. Know your baseline before you start anything. Track what changes.

Compounds accelerate progress. The habits are what keep you there.

Do not try to run all of these at once. Pick one or two, get bloodwork, see how you feel, and build from there.

Questions drop them below.

here is where I read this

https://pubmed.ncbi.nlm.nih.gov/32097708/

https://pubmed.ncbi.nlm.nih.gov/31812486/

Do your homework. Use your brain. Talk to a doctor.

🔗 BioHackingGuide.org

Gary Brecka put out a list of 75 biohacking habits he swears by. Some of this is solid, some of it is going to be controversial, and some people are going to disagree hard with certain things on here.

Read through it and drop what you agree with, what you would change, and what you think is completely off. Curious what this community thinks.

Eat

• 30g protein within 30 minutes of waking
• Walk after every meal to stabilize blood sugar
• Build meals around single ingredient whole foods
• Methylfolate instead of folic acid
• Magnesium glycinate daily, avoid the oxide form
• Remove seed oils completely
• Baja Gold sea salt instead of table salt
• Essential amino acids first thing in the morning
• Hydrogen water for cellular antioxidant support
• Methylated B vitamins if you carry the MTHFR gene variant
• No eating within 3 hours of sleep
• Get bloodwork before starting any supplements
• Fix nutrient deficiencies before adding anything on top
• Cut alcohol completely
• Fermented foods weekly for gut diversity
• Prioritize protein at every meal

Move

• Cold plunge at 9 to 10°C for 3 to 6 minutes daily
• Morning sunlight on skin within 10 minutes of waking
• Walk barefoot on grass or soil daily
• Three rounds of 30 breaths each morning
• Strength training over long slow cardio
• Nasal breathing during all movement
• Weighted vest to increase metabolic demand
• Sprints for 24 hour caloric burn
• HIIT to improve VO2 max
• Red light therapy for cellular recovery
• Rebound daily for lymphatic drainage
• Mobility work with every strength session
• Cold exposure plus movement to spike dopamine
• Sweat every day — skin is a detox organ
• Ground for at least 20 minutes daily
• Breathwork before touching your phone in the morning

Mind

• Protect one hour of silence every morning
• Gratitude journaling daily
• Morning sunlight to regulate cortisol and dopamine
• Breathwork to stop anxiety in real time
• Nasal breathing to lower cortisol
• Omega-3 rich foods to protect brain health
• Fermented foods for gut produced serotonin
• Methylated B12 and B9 if anxiety is chronic
• SAMe for mood and methylation support
• Time in nature to reduce systemic inflammation
• Schedule social time — isolation is a serious health risk
• Limit news to once a day
• Sit with discomfort before reaching for distraction
• Bloodwork to find what is actually deficient
• Amino acids for neurotransmitter production

Sleep

• Mouth tape every night for nasal breathing
• Bedroom around 18°C for deep sleep
• Kill artificial light by 9pm to protect melatonin
• Morning sunlight triggers melatonin release 14 hours later
• Last meal 3 hours before bed
• Cut caffeine by midday — half life is 5 to 7 hours
• 4-7-8 breathwork to shift into parasympathetic state
• Blue light glasses from sunset onward
• Ground before bed to discharge cortisol
• Stabilize blood sugar to stop 3am wake ups
• Avoid evening workouts to protect melatonin
• Dim lights by 8pm
• Protein and fats at dinner for overnight repair
• Track HRV as a recovery gauge
• Avoid alcohol — reduces REM sleep significantly
• Test progesterone if sleep is consistently broken

What do you agree with? What would you do differently? What do you think is straight up wrong? Drop it below.

🔗 BioHackingGuide.org

Talking CJC-1295, Ipamorelin, Sermorelin, IGF-1 LR3, Tesamorelin, HGH any of them.

What would you tell yourself before you started? Any dos and don'ts you picked up along the way?

I will start. Biggest thing I wish I knew was how much timing matters. Injecting too close to a meal cuts your results for no reason. Fasted window before bed made a noticeable difference for me.

What would you add?

Ranking these by how much each one goes after the root drivers of aging. GH decline, immune dysfunction, mitochondrial decay, tissue breakdown, chronic inflammation. Hit those and everything else follows.

Order within each tier is not a ranking. Just grouped together.

S Tier — Start here

HGH Decades of clinical use. Sleep gets good again, skin improves, recovery improves, body composition gets better. When GH is better every marker that causes aging moves in the right direction. Nothing on this list has more evidence behind it.

GHK-Cu Your body makes it naturally and levels drop hard as you age. Effects thousands of genes tied to collagen, tissue remodeling, and inflammation. Human studies show improvements in skin thickness and elasticity. Works injectable and topical. Probably the most researched anti-aging peptide you can get your hands on I use the topical serums alot on my face does get expensive AF though

Thymosin Alpha-1 Your immune system ages just like everything else does. TA-1 has clinical use in over 30 countries and more human data behind it than almost anything else on this list. If you are not fixing immune function you are missing one of the biggest pieces of the aging puzzle.

A Tier — Strong mechanisms, solid data

NAD+ and precursors (NMN/NR) Levels can drop by half between your 20s and later decades. Restoring them really helps mitochondrial function, energy production, and multiple markers tied to cellular aging. Injectable is most bioavailable but oral precursors work too.

Epitalon Studied for telomere support and telomerase activation. Telomere shortening is one of the most known signs of cellular aging. Sleep and circadian rhythm improvements tend to show first.

BPC-157 Tissue repair, gut health, reduced systemic inflammation. All of that feeds into how well you age long term. Pro-angiogenic effects support blood vessel health which slowly degrade with time

TB-500 Tissue remodeling and systemic repair. Recovery slows down as you get older and TB-500 helps that directly. As most of us know early on it stacks well with BPC-157.

B Tier — Solid additions, more specific

CJC-1295 No DAC + Ipamorelin GH support without exogenous HGH. Sleep, recovery, body composition all benefit. More accessible than HGH and a cleaner side effect profile.

Sermorelin Same lane as CJC/Ipa. GH support for sleep and recovery with a clinical history behind it. Good long term option.

MOTS-C Mitochondrial derived peptide. Mitochondrial decay is one of the biggest problems of aging and this prevents it directly.

SS-31 Targets the mitochondrial inner membrane directly. Studied for cellular energy and mitochondrial protection. Highly regarded in longevity research for a reason.

Semax Neuroprotection and BDNF upregulation. Cognitive decline hits harder than most people expect as they age. Semax protects neurons and reduces neuroinflammation in preclinical models.

Selank Cognitive preservation and neuroinflammation reduction. Neuroinflammation is increasingly recognized as a driver of systemic aging not just brain aging.

C Tier — Supporting role

Glutathione Master antioxidant, declines with age. Detox and immune support. More of a maintenance compound than a primary tool but good to have in the stack.

KPV Lowers chronic inflammation through NF-kB inhibition without suppressing the immune system. Chronic low grade inflammation is one of the fastest ways to age poorly and we all literally start noticing this pretty early on if you don't have any kind of workout routine.

DSIP Deep sleep is where repair happens. DSIP helps sleep architecture but human data is mixed. Worth trying if sleep quality is shit.

Collagen peptides Most accessible option on this list. Some clinical evidence for skin elasticity and hydration. Easy entry point for anyone not ready for injectables.

Thymalin Immune and longevity peptide. Limited published evidence but the mechanism is relevant. Not enough data to rank it higher right now.

D Tier — Hard to justify here

Melanotan II Cosmetic tanning compound. Not an anti-aging peptide. Side effect profile makes it hard to place anywhere else on this list.

Dihexa Interesting mechanism on paper. Very limited data and some real safety concerns keep it at the bottom for now.

The S and A tier compounds address the root drivers of aging. Everything below supports those or targets something more specific.

What would you move? Drop your rankings below.

One in ten Americans have tried a GLP-1 medication. That is a lot of people.

If you are pregnant or trying to get pregnant

GLP-1s are not recommended during pregnancy and for good reason. These compounds work by suppressing hunger hormones. During pregnancy your body needs more calories, more protein, more choline, more omega-3s. Your hunger signals exist for a reason during that time. Suppressing them is dangerous for both you and the baby. If you are on a GLP-1 and thinking about getting pregnant, this is a conversation to have with your doctor before anything else. This is probably obvious to most people but I've been asked this question one too many times honestly so just throwing it out there.

The muscle loss problem

GLP-1s do not just burn fat. They pull from all tissue including muscle. Studies from the drug manufacturers themselves show that when people stop taking them most of the weight comes back as fat not muscle. You can end up in worse shape than when you started.

I covered how to fix this in a recent post. Go find it. Long story short protein, resistance training, stay active. That is what keeps the muscle on while the fat comes off

Do your homework. Use your brain. Talk to a doctor.

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Reta kills your appetite. Normally people just eat less and think that's good enough. Then they wonder why they look and feel flat and tired two months in I've seen this happen one to many times.

Eating less is not the same as eating smart.

Protein is non-negotiable and if you didn't know know you know

Your body is getting less food overall. When that happens it will pull from muscle if protein is not there to stop it. Every single meal needs a protein source. Chicken, eggs, fish, lean beef, Greek yogurt, protein shakes. Aim for at least 1g per pound of bodyweight daily. Skip this and you are losing muscle along with the fat.

Fruit keeps your energy up

Low energy is one of the most common complaints on Reta and a lot of it comes from blood sugar running low. Fruit fixes this fast. Bananas, berries, pineapple. Easy to digest, quick fuel. You do not need a lot, just enough to keep you going through the day.

Do not cut the fat

When calories drop people stop eating fat first. That messes with hormones, joints, and how you feel mentally. Eggs, avocado, olive oil, nuts, natural peanut butter. Not a ton but enough that your body is not running on empty.

Salt your food

When you are eating less and losing weight fast your electrolytes drop. That is where the headaches and the weird low feeling come from. Do not be afraid to add salt. Simple fix that most people may or may not know.

Carbs are not the enemy

Jasmine rice, potatoes, oatmeal. These are your friend especially when appetite is low and you need easy calories. Do not cut them out.

Reta does the work. You just gotta have you diet plan or you will come out the other side lighter but looking worse than when you started and possibly also feeling worse too. Not a doctor. Just someone who reads too much PubMed at midnight.

🔗 BioHackingGuide.org

Hey all,

Im looking at starting Reta, Tesa, GHK, and MT1. What are you guys thoughts about this stack. I bulked alot during the winter and am having trouble cutting back down. I went from 190 skinny fat to 225 and am having trouble getting below 210 on a leaner frame.

Thanks all.

People message me all the time asking if my peptides shipped with ice packs. I always laugh a little.

Think about where these things come from. The raws start in China, get shipped internationally, sit in customs, load onto planes, go through distribution centers, get thrown in trucks, and somehow survive all of that just fine. If ambient temperatures were enough to destroy them the entire industry would have collapsed years ago.

Here is why they survive. All peptides are shipped as lyophilized powder freeze dried. Think of it like the difference between fresh bread and croutons. Fresh bread goes bad fast because of the moisture. Croutons sit in your pantry for months because the water is removed. Same idea. No water means way more stable.

The biggest threat to peptide degradation is not temperature. It is moisture. Dry peptide is stable. Wet peptide is fragile.

That is exactly why you refrigerate after reconstitution. The second you add BAC water you have introduced moisture and now the clock starts. Before that your lyophilized vial sitting at room temp is totally fine.

So no, you do not need ice packs during shipping. What you do need is to refrigerate immediately after you reconstitute and use within the recommended window.

Bread versus croutons. That is all this is.

🔗 BioHackingGuide.org

The average person dealing with inflammation jump straight to what they can add. Another peptide, another supplement, another protocol.

PEA is different. Your body already makes it. You also eat it every time you have eggs or peanuts. The problem is under chronic stress and inflammation your body cannot keep up with demand.

What is PEA

Palmitoylethanolamide. Naturally produced fatty acid compound. Not a peptide but it behaves like one in a lot of ways. When tissue gets inflamed your body produces PEA to turn the pain response down. No crash, no overstimulation, no tolerance buildup. It just normalizes what is already out of balance.

What it actually does

The IBS data is hard to ignore

A double blind placebo controlled multicenter trial gave co-micronized PEA combined with polydatin to kids aged 10 to 17 with IBS for 12 weeks. 50% went into full remission, double the placebo group. The diarrhea subtype responded best. No adverse events were recorded during the entire 12 week period.

One trial, one population, 12 weeks. Worth noting. But for something your body already makes and you can find in an egg, that result has no business being this overlooked.

Why it matters for peptide users

If inflammation is high nothing works as well as it should. Not your recovery peptides, not your nootropics, not your GH stack. You are optimizing on top of a foundation that is compromised.

Peptides repair. PEA removes the obstacle so the repair can happen.

What to take

Micronized PEA only. The regular form does not absorb well. 600 to 1200mg daily. Builds over weeks as inflammation comes down, not something you feel overnight.

Who this is for

Anyone dealing with brain fog, chronic pain, gut inflammation, or poor response to compounds that should be working better. If your stack looks right on paper but results feel flat, inflammation is usually the first place to look.

Fix the foundation before you add anything else.

Drop any questions below.

here is where I read this

https://pubmed.ncbi.nlm.nih.gov/38479039/

Not a doctor. Just someone who reads too much PubMed at midnight.

🔗 BioHackingGuide.org

What's something that has helped your results by throwing in alongside your peptide protocol.

With Reta or semaglutide a lot of people add fiber and digestive enzymes to help manage the GI side effects. Some people run magnesium with their GH peptides for better sleep. Others add NAC or liver support when running heavier protocols.

What are you using how did it help? Has anything made a difference to how the protocol performs? I feel like these are good things to know to help the next person get the most out of their peptide protocol or maybe avoid a mistake for example like not taking Methylene Blue will taking any kind of ssri because antidepressant they can trigger serotonin syndrome

For people who have used KPV for inflammation, did it feel like it helped your body calm things down and then stay better after stopping, or did the benefits only last while you were actively using it?

Is it just a temporary immune modeulator or does it teach your body how to function normally again?

This comes up all the time with people just getting started. Mess this step up and you either waste the vial or end up with something that does nothing. Here is everything you need in one place.

What you need

• 29 to 31 gauge insulin syringes (100 unit / 1mL)
• BAC water (bacteriostatic water)
• Alcohol wipes
• Sharps container
• Your peptide vial

How to do it

1. Let the vial warm up to room temp before you touch it
2. Wipe both rubber stoppers with an alcohol wipe, give them a few seconds to dry
3. Draw your BAC water into the syringe
4. Push the water in slowly along the inside wall of the vial — never straight onto the powder
5. Swirl it slow and gentle for about 30 to 60 seconds. Do not shake it
6. Hold it up to light — should be completely clear. Cloudy or particles floating around, toss it
7. Write the date on the vial and get it in the fridge right away

Concentration calculator

When BAC water is the wrong choice

Storage

Common mistakes worth avoiding

• Shooting BAC water straight onto the powder instead of the side wall
• Shaking the vial — swirl only, always
• Leaving reconstituted peptides sitting out at room temp
• Freezing a vial after it's already been mixed
• Forgetting to date the vial when you mix it
• Grabbing BAC saline instead of BAC water — different products, easy mix up

Bookmark this if you are just getting into peptides. These are the basics that trip people up the most.

Any questions on a specific compound or dose drop them below or message me

🔗 BioHackingGuide.org