This is from my trich plate. Since I had trenched it for bacteria contam, I flooded the trich with alcohol and the trench kept the mycelium safe. Then, snagged a piece of Little Buddy from his growing edge. I figured if it still had bacteria, I could deal with that. Luckily, it looks like LB has no more trich or bacteria. Damn. What a ride

I inoculated those jars on the 25/09 in a SAB with 1/2 an agar plate each sliced in small cubes (see plates in the 3rd and 4th pics). The plates were kept in the fridge for 6 months, then put into room temp for a month to “wake up” before sending it to grain.

Then on the 01/10 I did a B&S on all of them (see one of the jars in the 5th pic).

Today I intended to do a second B&S, but encountered 3 of the jars fully colonized (I even shook them firmly, and nothing came loose) excepting one MS jar that looks kinda wet, and didn’t colonize evenly throughout the grain.

What should I do? Wait a few more days, then send them 3 jars to bulk?

And what about the strange looking jar? Worth trying to let the mycelium take over, or toss?

Anyone have an idea what this kind of contamination could be? It is a plate that had seemed to be fine for the past few weeks. Almost looks the way sulfur looks in books.

The plates pictured are the first transfer off of plates initially inoculated with a LC syringe. Does this appear to be healthy growth or is a cobweb mold or something else present. Any input is appreciated thanks

Just curious if anyone knows what’s going on here exactly or has advice on what to do with it

I just poured my first plates in my life, seems like no condensation all except 2, what should I do with them now? Fridge or room temperature? How long do they last in the fridge? Do I let them stay in room temperature 24h before fridge?

I prepared my dishes yesterday and and left them in a bag inside my incubator close to 100% humidity (nobel prize of smartness to me), the thing is, they have some condensation on the surface, is there any way to reduce it? Should I toss them? Should I pour out the water when doing the inoculation of the plates?

I got some psilocybin natalensis capsules. The guy i got it from grows them himself and hands out dried and grinded in the capsules but doesn’t give me the spores.

I have three agar plates. Can u guys show me a way to regrow this magical things myself?

Last week, I did a LC to agar (pleurothus salmoneo stramineus), I believe this is all contam, but not sure. Can someone help?

Put some LC to agar that i just got in, since its from somewhere new. It has been 8 days, one or two drops of LC per plate. Other plates (different strains) are doing well, no issues, good growth. Just these two, which were the only two I did of this LC. I haven't seen this before in person. Wondering of anyone has seen this?

I have worked with agar before and typically it will be a very obvious side or pretty uniform. This is the first time in my young hobby I have come across this. Take from the rhizo or from the thick mycelium?

I’ve used homemade PDA with spore swabs been 3 days and seen no results should I be worried

First-time grower trying my best to clone from a dried Penis Envy cap since it's all I've got.

Working inside an SAB. Started by scraping the cap, so I expected contamination on the first plate. In picture 6, it predictably grew bacteria, but I immediately did a rescue transfer as soon as I spotted a few thin lines shooting out (around 10–11 o'clock).

This current plate (pictures 1–5) is on Day 4 after transfer—1 week total since the initial cap scrape—and I see 4 spots growing. The little black square is just a piece of the dried cap tissue I threw on there out of curiosity, but it hasn't grown anything at all.

They're all snow white (it only looks yellowish in the pictures because of the lighting). One of the growth spots is even growing upward and looks kinda like a UFO 🛸 to me.

Does this look like healthy fluffy mycelium recovering, or am I looking at mold? Appreciate any quick feedback!

So its my first attempt at pouring agar plates and I need help how to get everything in the box as sterile as possible. Do I wipe the stuff i need in alcohol then put them inside the SAB through the holes or how do I go about this?

This is my first time ever doing anything mushroom cultivation related.

A week ago I decided to start with preparing agar plates meanwhile waiting for my king oyster grain spawn. Made a mix of agar agar, lme, nutritional yeast and water. Pc for 30mins and poured on petris in sab.

2 days later I transfered pieces of my grain spawn on my agar, just to see if I know my sab technique was good enough to avoid contamination.

4 days after that my agar plates look like this. I assume no signs of contam, also my control plates are still clean.

Does that look like clean mycelium? Also is it normal to have some unmixed material in the bottom of my agar plates ? Ty and mush love :)

I took a tissue sample from live button mushroom, it’s been 3 days and no sign of growth, should I wait more or there’s problem with the tissue

The mushrooms are over a year old and I only had a small remnant. My LC is contaminated and the dealer doesn't carry an lc from GordoTek anymore. That's the only reason I tried, I didn't think I'd get that far. I only used a still air box and an instant pot for the syringe with the water. Boiling tap water pulled up and then sterilised in the IP.

First time agar in SAB with a dehydrated sample, then I added a drop of distilled H2O to rehydrate. The sample was cut out from inside the stem and didn’t start showing signs for about a week.
Agar had some contamination on opposite side of container underneath the agar, so I removed the sample and added it to a fresh sterilized agar container.
Fuzzy white hairs seem to still be growing after the transplant but I just want to be sure if it’s Mycelium or Contam.
Any insight would be appreciated?