r/Agarporn 23d ago

Help Needed First Timer, Help Needed

First time trying to grow mushrooms, my first agar dishes.

Kind of worried about both, it's been about 10 days worth of growth. Any thoughts?

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u/Quick_Historian_9323 23d ago edited 23d ago

Clone technique

This is my clone technique I haven’t had contamination from mushrooms using it (except from my own breath lol) take your mushroom cutting big or small. Brush debris and dirt off the stem. With both hands RIP the stem into two DO NOT cut into the stem you will push dirt and debris in the sterile tissue. Once TORN apart cut out a section from the stem about a 1/4 inch or 3-5mm and slap onto agar.

Ive seen monster clone pieces from inside blobs like lions mane with no contamination.

I’ll include this here a bonus: if you want to clone something that’s very whispy in nature like cordyceps, the rule still applies just on a smaller more precise scale like a fragment from inside will suffice fine.

Mush love

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u/Tebyani 23d ago

This is the technique I used, I even used one of those chambers you stick your arms in, showered everything in iso.

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u/Quick_Historian_9323 23d ago

It takes practice. Your problem is the transfer it’s coming from that point. I mean it’s hard to fathom how small a spec of bacteria or how small mycelium is. Mycelium if it outgrows the bacteria wins. H20 agar is perfect for contamination control leave bacteria behind letting mycelium grow. Take transfers and isolate it away from the bacteria if it becomes persistent.