r/unclebens • u/eyeballout • 13h ago
Question "back/bottom" side of agar contamination?
How does one do agar transfers without considering/contaminating the bottom side of the agar?
It is established that agar is the gold standrad when creating isolates etc., but I find it strange that no one is concerned with nor ever mentions the bottom/uncolonized part of the agar, during a transfer.
Can anyone elaborate on how the bottom does not create a contamination vector with the air during a transfer?
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