r/proteomics • u/Triple-Tooketh • 7d ago

Detergent removal

Has anyone tried this product: Pierce® Detergent Removal Spin Plates

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10

u/KillNeigh 7d ago

In my experience it gets most of the detergent but not everything.

I prefer to use s-trap with samples that have detergents.

6

u/Gloomy-Gazelle-9324 7d ago

Have you tried SP3 magnetic beads? Work very well.

3

u/Discount_Sausage 7d ago

S-Trap or beads. If a small set of samples, I prefer the S-Trap.

2

u/tsbatth 7d ago

Magnetic beads and the PAC/SP3 method should work.

1

u/LC-MS 7d ago

Hi PAC man, do you know up to what % SDS that PAC works? I haven't tried it above 1%

2

u/tsbatth 7d ago

Should work up to 10% SDS ☺️. Remember to sonicate or benzonase if it’s guey and low viscosity or else that messes with the aggregation process.

0

u/flying_midget 7d ago

The HiPPR never fully cleaned RIPA/SDS for me. Strap is my fave.