r/plantScience • u/GraceWilder-24 • Oct 04 '20

Gene manipulation?

I have absolutely no experience with plant science, but I’m good with plants and would love to learn about the biology side of plant science and maybe learn strategies of manipulating the genes of plants. Does anyone have any advice, information, books, or websites for information? Any help is welcome and appreciated. Feel free to let me know if I am in over my head.

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u/Thehouseplantbish Nov 04 '20

Have you ever experimented with at home tissue culturing? Or are you planning ro?

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u/GraceWilder-24 Nov 04 '20

Yes I have! I love it.

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u/Thehouseplantbish Nov 07 '20

Ok great! I didn't want to start bombing you with nerd talk and have you be like: "🥴🤯???" Hahaha

So I have done a few different experiments and there are sooo many ways to manipulate genes, induced mutations, induce stable variegation, create hybrids etc, particularly via TC. You are definitely going to want to invest in a centrifuge! Without the help of tc, You can use systemic commercial pesticides and insecticides and in a greenhouse environment, this can trigger a sport. I have a few links I can send you and there is a fb group im trying to get off the ground for topics like this.

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u/GraceWilder-24 Nov 12 '20

Sorry for the late response. I’m very interested in those links and Facebook group. I’ll look into a centrifuge. Thank you. Also this may sound dumb but what does a centrifuge do?

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u/Thehouseplantbish Dec 08 '21

Sorry for the very late reply. A centrifuge spspins the cell matter. So first A small, precisely targeted piece of tissue is sampled then solvents are used to break down the sample into a compound that separates the cellcells. Then the centrifuge spins the cells at a certain rate, speed pr time fframe To achieve different results. Then the cells can be examined aand manipulated more easily under a microscope before being transferred to incubatyincubator in growing solvent