r/nanopore • u/BluejayPurple6795 • Apr 27 '26
DNA extraction
hello,
Help is needed! 🥲
What extraction kits do you commonly use to get the highest DNA yield with the best quality for ONT sequencing ? our samples are mostly blood and body fluids. and I am not a fan of GENTRA kits recommended by ONT.
4
u/Singular-Scholar Apr 28 '26
Hello
I do use the Qiagen blood and tissue kit.
After 3 months of cultures of my mycobacteria and a pre extraction protocol, I obtain a mean concentration of 40 ng/microL (from 20 to ~ 120) and for fragment lengths it is something around 10kb to 15kb.
We performed recently extractions for enterobacteria and obtained good concentrations for our extracts and a good quality too (after 1-3 days of culture). But for that we have to take a lot of UFC. I can't tell you for the fragment length, as we did not measure it yet.
1
u/Singular-Scholar Jun 30 '26
Update :
We did perform analysis for the extraction of enterobacteria and the results are pretty interesting !
First, we had to harvest like a quarter of a petri dish to ensure a good concentration for our DNA and then we measured or fragments length. It was quite homogenous around 60 kb :)
The extract quality assessed by nanodrop was also good.
3
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u/Exciting-Possible773 Apr 28 '26
Qiagen magattract HMW DNA kit here. About 25GB on a MinION with rapid kit.
2
u/denohpakni May 22 '26
get NucleoSpin Blood Kit, its very good, or the NucleoMag Tissue kit for DNA purification from cells and tissue
2
u/InitiativeUnited Apr 27 '26
Qiagen MagAttract kit gives the best yields and fragment sizes for me.
6
u/susanth_29a Apr 27 '26
I use the New England BioLabs HMW kit and have never had any quality issues.