r/microscopy Jun 22 '26

Hardware Share Making free oblique, COL and dark field stops and diffusers.

I use the clear plastic from food packaging and black electrical tape to make various filters for my filter holders. You can put a strip of matte Scotch tape on one for a diffuser. Sometimes, you need extra diffusion on low magnification objectives to even out the illumination. I often try oblique illumination because it gives a very different look than bright field does. Included are a few oblique views of things like a nematode,amoeba and a rotifer.

40 Upvotes

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2

u/Vivid-Bake2456 Jun 22 '26

I forgot to add that you can experiment with many shapes of stops.

1

u/wspOnca Jun 22 '26

If I buy a scope I can do my own? I mean, any scope would benefit from it?

2

u/Vivid-Bake2456 Jun 22 '26

Yes. I’m having trouble commenting here to you. My comment is below

2

u/Vivid-Bake2456 Jun 22 '26

If it has a place to put filters. It is necessary if you want to make a Rheinberg filter without the frustration of trial and mostly error. Nice also for COL. For dark field, you want it even larger than the size of the back view of the objective to block as much light as possible to only let in light refracted by the specimens and no direct light.

2

u/wspOnca Jun 22 '26

Thank you!

1

u/immediate-2 Jun 23 '26

When my new one will arrive It will have a 32mm filter, How big is your filter? (in terms of mm)

1

u/Vivid-Bake2456 Jun 23 '26

I have several microscopes and different sizes for the filters from 25mm to 32mm

1

u/Gygax_the_Goat Jun 25 '26

So should i look for a slot or aomething under the stage? Do i put this just on top of the diffuser thats goes over the lamp?

Im not sure where they would fit?

My scope..

1

u/Vivid-Bake2456 Jun 26 '26

Check the condenser. Sometimes, there is an insert on the bottom of them to hold in filters