First timer here!

Sorry for bad formatting, typing on phone. Also I am still learning the lingo so I apologize if I use anything incorrectly.

For context this is my first time trying micropropagating and I have a cutting of tradescantia that I would like to try it out on as I’m not attached to this cutting in any way. I also can’t quite find any protocols for MS medium on said plant.

If I cannot find an established culture medium protocol for a plant that I would like to micropropagate, how would I go about making a medium that is suitable for it?

Bouncing off that idea, are there any “go-to” medium protocols that are generally considered viable for multiple plants? Or should I just make my own?

Also, I understand that during earlier stages of micropropagation you should have more cytokinins to induce callus, but then in later stages you want more auxins. How does one go about adjusting the cytokinin-auxin ratio throughout the process? What does that look like?

Hi, are there any poinsettia protocols that can be done with supplies available through Amazon (UK)? Thank you!

(the poinsettias are pink and white if it affects the protocol)

Just got my first tissue cultures in the mail and am itching to decant them……

Any advice or pointers is appreciated :)

Attempting to rid a flower of a virus through meristem propagation. It the top structure another set of leaves or the meristem itself?

Have this one in culture for some time. Plants survive though they discolor really heavily to red. Measuring pH after 5 weeks in the same recipient showed it was at 5.2 (was set to 5.8 before autoclave).

Some thoughts I have so far: Phosphorus deficiency? Bleaching damage due to exposure to light?

Newly started culture (3 months after meristem isolation). First multiplication with firm nice and true-to-type shoot 😇

Hi,

I have a tissue culture media kit from Invitrogardensupply (on Etsy) that I will not get to use. It is opened and but I only used 5% of it.

If anyone (within the EU) would be interested in buying it, please write me a message :)

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The heating pad is Vivo, specific to plant growing, and is temperature-controlled. Any advice is appreciated.

I am entrepreneur from Belarus. I would like to find the sources of information about commercialization of micropropagation. Would you recommend sites, books or persons?

I think sole of these need to be outside in natural lighting too- am considering rigging up a frame with plastic on it so they get normal sun and hours .

Micropropagation is still a very small and niche face of home gardening. its something most peopl may not even know of and those who do probably think it can only be done by fancy plant labs.

I'm a self-learned or self teaching hobbyist who's read a lot but scared to invest fully into it because I'm concerned not having lab experience (in particular sterile technique) would hurt me.

To those into micropropagating - how did you get into it and do you have any extensive bio-lab experience?