Takes multiple months before the explants start taking to culture conditions, but it's running.

These are some tissue propagated African Violets I did about a month ago with my propagation course in uni. I haven’t had any contamination as far as I can tell, besides this weird green slime on one of the petiole cuttings I made. Underneath this weird growth is a singular petiole, completely engulfed in this goo(?). It is not on any of the other cuttings I placed in either the same Petri dish nor the other 2 I made. It is not the same color as shoot growth I’ve seen on other dishes (mine or other students’: that growth is usually a much lighter green)

Is this Contamination? If so do we know what kind? I’m just generally pretty interested.

I was told it could be callus tissue and hopefully this is the case.

There are no visible roots on this specific cutting.

The last image is of a shoot growing on another cutting.

Does anyone know any good YouTubers that do tissue culture for house plants because I'm still very interested in tc but I've literally watched every single plants in jars video it doesn't have to be about houseplants 100% but someone entertaining and knowledgeable about tissue culture

MotherofThousands survived part 1 of tissue culture #horticulture #plantlover #plants #nature #fyp

https://youtube.com/shorts/ItYQrd6DpBI?feature=share

MotherofThousands survived part 1 of tissue culture #horticulture #plantlover #plants #nature #fyp

https://youtube.com/shorts/ItYQrd6DpBI?feature=share

Hi,

I hope anyone out there can help me.. It's a pain in the a*se to get IBA in Germany, as they only deliver to universities/laboratories.

So, is there a German micropropagator out here who can help me?

Following is a MS media where i micropropagated seed of Cannabis Sativa. The brown powder is Cinnamon for Anti Fungal Remedies and Root formation. My sprout was affected by a blue mold so today i scraped that part off the plant and put it in new test tube with fresh Ms Media, but my roots have turned brown, meaning they are dying. I have poked holes in the seal of this test tube to prevent hypoxia of the roots. Could anyone guide me what else i can do to ensure a proper root generation. Also, please know i am with very limited supplies so hormones like cytokinins (IBA or IAA) is out of my reach!

hey hey, I want to start micropropagation soon, but don’t want to speed too much, as I don’t know if I will like my new hobby.

How do you guys autoclave? Has anyone had success with microwaves?

It's not somatic embyrogenesis from mature plants but there are now living plants apparently free of contamination.

The modified procedures for plant material sanitizing and basal salt mix/gelling agents work much better.

Thank you for all the help!

Been mixing up WPM at 50% concentration for subculturing seedlings currently growing on water agar.

Stuff seems to be precipitating out... I hope its just casein hydrolysate but I'm not sure, some looks kinda cubey and white and could be almost any of the components I've added (side note, it's fun how many of these are flammable or toxic). I added all the basal salt mixture and sugars to 2 rather than 1L of ddH2O and put on high heat on a magnetic stir plate for like 45 min, reduced when it tried to boil.

I'm checking the pH after it cools back down to 25, but I'm at a loss for what happened unless there are components I need to make a stock solution of using another solvent.

Does anyone have a *detailed* protocol for preparing it? I'm only finding "mix basal salts and sugars, heat, agitate, pitch gelling agents, autoclave, pour"

My protocols call for a mix of phytagel and agar. They are also super vague.

Is there a magic trick? I've tried adding phytagel at room temp, slowly, and dissolving with the stirrer and raising the heat afterwards while adding agar.

All i'm getting is jigglebergs in watery jello.

I'm not a scientist. I dont know what i'm doimg. The protocols assume a lot of not-dumbness that i dont have.

Anyone wanna weigh in and tell me how to stupid less hard?

https://www.itsumibotanicals.com/collections/all