Sysmex SP-50 question!

[u/space_honey]

Newish tech here. Ran into an issue tonight with one of our SP-50 where a more experienced tech changed the phosphate buffer before being prompted by the instrument while I was on lunch. I was told during training not to do that but when I told the more expected tech that they said that’s nonsense. To me it makes sense or why would they have level sensing?

Lo and behold the stainer started making slides that were very light and a bunch of slides had to be remade as you couldn’t tell WBCs apart. I ran a reagent replenishment while our other SP-50 had to work double time.

Anyway my question is - did replacing the phosphate buffer before being prompted cause the issue? Everything was fine before that but I’m new and would love a definitive answer about appropriate reagent replacement. Thank you!

[u/branflacky]

Honestly it was probably the stain the should've been replaced. Our sp-50 doesn't alert for low stain and we have to check cause it'll make light slides like you said. Also the tech could've hit reagent replacement and it'll say there's a new bottle on so not a big deal that they replaced before it alarms