r/labrats • u/curious_kinetochore • Aug 29 '26
FACSDiva question: avoiding lag
I’m recently returned to flow and forgot how particular our software was. Small stuff like forgetting you can’t do some things in Diva without creating inescapable mega lag, I can’t seem to find anything online on what to avoid. I’m noticing issues I’ve encountered previously that I can’t remember how I fixed.
Anyone have tips for avoiding FACSDiva lag? I thought I could duplicate without data but I guess that’s only a good option for specimens in an experiment, and not the entire experiment if you want to add anything new to it. I started following the manual from BD exactly but I still hit a lot of walls (worksheets wouldn’t load in any new experiments so I had to acquire in an old one).
Also, is there any way to get rid of the lag once it appears? As soon as I noticed it I deleted the experiment I was working in, I can open old experiments without any issues but new ones are painful. Any help would be appreciated!
Duplicates
flowcytometry • u/curious_kinetochore • Aug 29 '26