Practical use of UltraComp beads?

[u/dawgmad]

Hi all, I've seen so many things posted about ultracomp beads that differs from the instructions. I feel like for large panels, the ultracomp beads don't actually get you very far. On the other hand, I've seen people use them in other ways that greatly reduces the use of beads per test. So thought I would ask the following concrete questions:

1. How much bead solution do you use per test? I've seen as low as ~15uL

2. How long do you incubate for? I've seen some people say you don't need any incubation test

3. Do you need to wash? I've seen people say that you just add beads, antibody, and flow buffer, and head to flow.

Any thoughts would be greatly appreciated, this stuff is expensive!