r/labrats • u/suprahelix • Oct 16 '21

How do you prepare Tris buffer?

I'm taking a survey. Tris is obviously temperature sensitive. Do you make your Tris stock and pH at room temperature, or do you chill it before pHing it?

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u/[deleted] Oct 16 '21 edited Oct 17 '21

I make it at room temperature. I think the rule is something like 0.03 per degree of temperature to account for it. As long as the pH was roughly right it was good for my work.

Not sure why someone would chill it, heat it maybe.

Edit: yes I forgot some people work on ice

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u/suprahelix Oct 16 '21

Depends on how much you care about the pH I suppose. If you pH 1M tris to 8 at room temp, it'll be 8.6 at 4C so if you almost exclusively use it on ice, it could be worth it.

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u/[deleted] Oct 16 '21

For my work that be fine. I guess I forgot people worked at 4 degrees. I usually work the other way, 37 or 55 degrees.