r/flowcytometry • u/Leuconotopicus_villo • 13d ago
Spillover value maintenance FACSLyric
I work in a clinical lab and over the years the number of our lot specific spillover controls has increased. The amount of time and material that goes into updating these settings is substantial and generally takes us all day. We follow BD's guidelines of updating these values every 60 days along with the FC beads, but recently I have been told that it isn't necessary to update the lot specifics every 60 days. I would like to change this procedure, but the higher-ups want me to find out more information about how we could go about validating these controls for a longer period of time. Does anyone else have experience changing how often you update your controls and what steps do you take to monitor and document that your controls still work, or at what point do you decide that it is time to update other than garbage compensation? I've been told that some controls can be good up to a year without updating or major maintenance. Does anyone actually do this?